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A Comparison of Fresenius Com.Tec Cell and Spectra Optia Cell Separators for Autologous and Allogeneic Stem Cell Collections: Single Center Experience

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Indian Journal of Hematology and Blood Transfusion Aims and scope Submit manuscript

Abstract

Peripheral blood is the prefered source for hematopoietic stem cells for hematopoietic stem cell transplantation. The efficiency of peripheral blood stem cell (PBSC) collection can vary among devices. In this study we aimed to compare feasibility and effectivity of apheresis procedures of the different systems. Two apheresis systems [Com.Tec (Fresenius Healthcare) and Spectra Optia (Caridian BCT)] were used in our center for the collection of PBSCs for autologous and allogeneic transplantation. We retrospectively analysed 190 apheresis procedures performed in healthy donors and patients between June 2012 and November 2014 in Department of Hematology, Dokuz Eylul University. PBSCS were collected by Fresenius cell separator (64 procedure) or Spectra Optia cell separator (126 procedure). Mobilization treatments were G-CSF (26.8%), cyclophosphamide plus G-CSF (48.4%), prelixafor plus G-CSF (14.7%), ESHAP (10%) and others. Patient and donor characteristics (age, weight, volume processed, disease, mobilization regimes) were similar in Fresenius and Spectra Optia apheresis groups. Altough both collected PBSCs efficiently, the amount of CD34+ cell in product collected by Spectra Optia device was significantly higher (p < 0.05) and product volume was lower than Fresenius Com.Tec significantly (p < 0.05). “CD34+ collection efficiency” with Spectra Optia was significantly higher than Fresenius Com.Tec (CE2: 87%, 70%, p = 0.033) regarding all procedures. High collection efficiency and low product volume may be a significant characteristic of Spectra Optia device (mean 187 mL, product CD34+ cell: 1576 µL).

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References

  1. Grathwohl A, Baldomero H, Schmid O, Horisberger B, Bargetzi M, Urbano-Ispizua A (2005) Change in stem cell source for hematopoietic stem cell transplantation (HSCT) in Europe: a report of the EBMT activity survey 2003. Bone Marrow Transpl 36:575–590. https://doi.org/10.1038/sj.bmt.1705104

    Article  Google Scholar 

  2. Stem Cell Trialists Collaborative Group (2005) Allogeneic peripheral blood stem cell compared with bone marrow transplantation in the management of hematologic malignancies: an individual patient data meta-analysis of nine randomized trials. J Clin Oncol 23:5074–5087. https://doi.org/10.1200/JCO.2005.09.020

    Article  Google Scholar 

  3. Sohn SK, Kim JG, Baek JH, Lee KB (2004) Diverse clinical application using advantages of allogeneic peripheral blood stem cell transplantation. Int J Hematol 79:457–461. https://doi.org/10.1532/IJH97.A10313.4

    Article  PubMed  Google Scholar 

  4. Altuntaş F, Koçyigit I, Ozturk A, Kaynar L, Oztekin M, Solmaz M, Eser B, Cetin M, Unal A (2007) Comparison of the Fenwal Amicus and Fresenius Com.Tec cell separators for autologous peripheral blood progenitor cell collection. Transfus Apher Sci 36:159–167. https://doi.org/10.1016/j.transci.2006.10.008

    Article  PubMed  Google Scholar 

  5. Dihenescikova VR, Mistrik M, Martina J, Zwiewka M, Bizikova I, Batorova A (2015) Collection of peripheral hematopoietic stemprogenitor cells. Bratis Lek Listy 116:9–13. https://doi.org/10.4149/BLL.2015.002

    Article  Google Scholar 

  6. Adorno G, Del Proposto G, Palombi F, Bruno A, Ballatore G, Postorino M, Tednas A, Del Poeta G, Isacchi G, Amadori S (2004) Collection of peripheral progenitor cells: a comparison between Amicus and Cobe-Spectra blood cell separators. Transfus Apher Sci 30:131–136. https://doi.org/10.1016/jtransci.2003.10.007

    Article  PubMed  Google Scholar 

  7. Bachier C, Potter J, Potter G, Sugay R, Shaughnessy P, Chan K, Jude V, Madden R, LeMaistre C (2012) High white blood cell concentration in the peripheral blood stem cell product can induse seizures during infusion of autologous peripheral blood stem cells. Am Soc Blood Marrow Transpl 18:1055–1060. https://doi.org/10.1016/j.bbmt.2011.12.500

    Article  Google Scholar 

  8. Ozkan MC, Sahin F, Saydam G (2015) Peripheral blood stem cell mobilization from healthy donors. Transfus Apher Sci 53:13–16. https://doi.org/10.1016/j.transci.2015.05.008

    Article  PubMed  Google Scholar 

  9. Neyrinck M, Virielink H (2015) Calculations in apheresis. J Clin Apher 30:38–42. https://doi.org/10.1002/jca.21347

    Article  PubMed  Google Scholar 

  10. Kim SR, Choung HK, Kim DW, Sung KW, Kang ES (2011) Evaluation of a new cell separator for collection of peripheral blood CD34 + progenitor cells in pediatric patients. Transfusion 51:306–312. https://doi.org/10.1111/j.1537.2995.2010.02864

    Article  PubMed  Google Scholar 

  11. Bambi F, Faulkner LB, Azzari C, Gelli AM, Tamburini A, Tintori V, Lippi AA, Tucci F, Bernini G, Genovese F (1998) Pediatric peripheral blood progenitor cell collection: haemonetics MSC3P versus COBE Spectra versus Fresenius AS104. Transfusion 38:70–74

    Article  CAS  Google Scholar 

  12. Flommersfeld S, Backchoul T, Bein G, Wachtel A, Loechelt C, Sachs UJ (2013) A single center comparison between three different apheresis systems for autologous and allogenei stem cell collection. Transfus Apher Sci 49:428–433. https://doi.org/10.1016/jtransci.2013.06.002

    Article  PubMed  Google Scholar 

  13. Brauninger S, Bialleck H, Thorausch K, Felt T, Seifried E, Bonig H (2012) Allogeneic donor peripheral blood “stem cell” apheresis: prospective comparison of two apheresis systems. Transfusion 52:1137–1145. https://doi.org/10.1111/j.1537.2995.2011.03414

    Article  PubMed  Google Scholar 

  14. Brauninger S, Bialleck H, Thorausch K, Felt T, Seifried E, Bonig H (2011) Mobilized allogeneic peripheral stem/progenitor cell apheresis with Spectra Optia v. 5.0, a novel, automatic interface—controlled apheresis system: results from the first feasibility trial. Vox Sang 101:237–246. https://doi.org/10.1111/j.1423.0410.2011.01484

    Article  CAS  PubMed  Google Scholar 

  15. Wu F, Heng K, Salleh RB, Soh TG, Lee J et al (2012) Comparing peripheral blood stem cell collection using the COBE Spectra, Haemonetics MCS and Amicus. Trans Apher Sci 47:345–350. https://doi.org/10.1016/j.transci.2012.07.015

    Article  Google Scholar 

  16. Basquiera AL, Abichain P, Damonte JC et al (2006) The number of CD34 (+) cells in peripheral blood as a predictor of the CD34+ yield in patients going to autologus stem cell transplantation. J. Clin Apher 21:92–95. https://doi.org/10.1002/jca.20062

    Article  CAS  PubMed  Google Scholar 

  17. Mohle R, Murea S, Pforsich M et al (1996) Estimated of the progenitor cell yield in a Leucapheresis product by previous measurement of CD34+ cell in the peripheral blood. Vox Sang 71:90–96

    Article  CAS  Google Scholar 

  18. Fante CD, Perotti C, Viarengo G, Bellotti L, Parisi C, Marchesi A et al (2006) Clinical impact of a new automated system employed for perpheral blood stem cell collection. J. Clin Apher 21:227–232. https://doi.org/10.1002/jca.20102

    Article  PubMed  Google Scholar 

  19. Ford C, Chan K, Reilly W, Peterson F (2003) An evaluation of predictive factors for CD34 cell harvest yields from patients mobilized with cheomatherapy and growth factors. Transfusion 43:622–625. https://doi.org/10.1046/j.1537.2995.2003.0037

    Article  CAS  PubMed  Google Scholar 

  20. Schots R, Van Riet I, Damiaens S, Flament J, Lacor P, Staelens Y et al (1996) The absolute number of circulating CD34 cells predicts the number of hematopoetic stem cells than can be predict by apheresis. Bone Marrow Transpl 17:509–515

    CAS  Google Scholar 

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Authors and Affiliations

  1. Department of Hematology, Bozyaka Teaching and Research Hospital, Izmir, Turkey

    Serife Solmaz & Celal Acar

  2. Department of Hematology, Medical Park Hospital, Izmir, Turkey

    Selda Kahraman

  3. Department of Hematology, Fırat University Faculty of Medicine, Izmir, Turkey

    Omur Gokmen Sevindik

  4. Department of Hematology, Dokuz Eylul University Faculty of Medicine, Izmir, Turkey

    Munire Turkyilmaz, Inci Alacacioglu, Ozden Piskin, Mehmet Ali Ozcan, Hayri Guner Ozsan, Bulent Undar & Fatih Demirkan

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  1. Serife Solmaz
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Correspondence to Serife Solmaz.

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Solmaz, S., Kahraman, S., Sevindik, O.G. et al. A Comparison of Fresenius Com.Tec Cell and Spectra Optia Cell Separators for Autologous and Allogeneic Stem Cell Collections: Single Center Experience. Indian J Hematol Blood Transfus 34, 677–683 (2018). https://doi.org/10.1007/s12288-018-0922-4

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  • DOI: https://doi.org/10.1007/s12288-018-0922-4

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