The Inhibitory Effect of Epigallocatechin Gallate on the Viability of T Lymphoblastic Leukemia Cells is Associated with Increase of Caspase-3 Level and Fas Expression
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Acute lymphoblastic leukemia is the most prevalent cancer in children. Novel components to help struggle aggressive malignancies and overcome some side effects of conventional treatments could be a promising strategy. Epigallocatechingallate (EGCG), have attracted the attention of scientists for prevention or treatment of some cancers. Jurkat cells were incubated with the different concentrations of EGCG (30–100 µm) for 24, 48, and 72 h and cell viability was investigated using MTS test. Apoptosis and the level of caspase 3 alterations were evaluated using flowcytometry and expression of Fas by Real Time PCR. EGCG decreased viability of cells with an inhibition concentration (IC50) of 82.8 ± 3.1, 68.8 ± 4 and 59.7 ± 4.8 μM in 24,48 and 72 h. 50, 70 and 100 µM concentrations of EGCG induced apoptosis in about 31, 40 and 71% of the cells, respectively. The mean value of caspase 3 positive cells in the presence of 50, 70 and 100 µm concentrations of EGCG was 19.3 ± 2.9, 29.5 ± 3.1 and 61.2 ± 3.4 respectively compared to 7.8 ± 1.1 in control with a significant difference at 100 µm concentration. Treatment with EGCG for 48 h enhanced the expression of Fas reaching to a significant level at 100 µM concentration. EGCG is effective in decrease cell viability, apoptosis induction and enhancement of caspase 3 and Fas expression level in jurkat cells. A comprehensive understanding of molecular events and pharmacokinetics of the component and experiments in animal models are required for dose determination and its interaction with other components of combination chemotherapy.
KeywordsEGCG Jurkat cell line Apoptosis Caspase 3 Fas
The authors would like to thank the Deputy of Research and Technology of the Shahrekord University of Medical Sciences for financial support of the project with the Grant No. 1317.
- 4.Shirzad H, Taji F, Pourgheysari B, Raisi S, Rafieian KM (2012) Comparison of antitumour activities of heated and raw garlic extracts on fibrosarcoma in mice. J Babol Univ Med Sci 14(6):77–83Google Scholar
- 9.Adachi S, Nagao T, Ingolfsson HI, Maxfield FR, Andersen OS, Kopelovich L et al (2007) The inhibitory effect of (−)-epigallocatechin gallate on activation of the epidermal growth factor receptor is associated with altered lipid order in HT29 colon cancer cells. Cancer Res 67(13):6493–6501CrossRefPubMedGoogle Scholar
- 10.Rahimnejad T, Beshkar P, Shirzad H, Rafieiankopaei M, Safdari V, Asgarian Dehkordi N et al (2014) Effect of pterostilbene on cellular proliferation inhibition and induction of apoptosis in lymphoblastic leukemia cell line. J Babol Univ Med Sci 16(12):32–38Google Scholar
- 26.Hou Z, Sang S, You H, Lee MJ, Hong J, Chin KV et al (2005) Mechanism of action of (−)-epigallocatechin-3-gallate: auto-oxidation-dependent inactivation of epidermal growth factor receptor and direct effects on growth inhibition in human esophageal cancer KYSE 150 cells. Cancer Res 65(17):8049–8056CrossRefPubMedGoogle Scholar
- 34.Lin HY, Hou SC, Chen SC, Kao MC, Yu CC, Funayama S et al (2012) (−)-Epigallocatechin gallate induces Fas/CD95-mediated apoptosis through inhibiting constitutive and IL-6-induced JAK/STAT3 signaling in head and neck squamous cell carcinoma cells. J Agric Food Chem 60(10):2480–2489CrossRefPubMedGoogle Scholar
- 36.Fang CY, Wu CC, Hsu HY, Chuang HY, Huang SY, Tsai CH et al (2015) EGCG inhibits proliferation, invasiveness and tumor growth by up-regulation of adhesion molecules, suppression of gelatinases activity, and induction of apoptosis in nasopharyngeal carcinoma cells. Int J Mol Sci 16(2):2530–2558CrossRefPubMedPubMedCentralGoogle Scholar
- 37.Soltani A, Pourgheysari B, Shirzad H, Sourani Z (2016) Antiproliferative and apoptosis-inducing activities of thymoquinone in lymphoblastic leukemia cell line. Indian J Hematol Blood Transfus (Epub)Google Scholar