Predictors of Mononuclear Cell Yield in Patients Undergoing Autologous Mononuclear Stem Cell Therapy in Non-haemopoietic Degenerative Disorders

Original Article
  • 29 Downloads

Abstract

Cellular therapy outcomes are influenced by cellular composition of the product. We analyzed the cellular profiles (TNC, MNC and CD34+ cells) of patients receiving mononuclear cell therapy in terms of age, gender, BMI, pre-harvest haematological counts and clinical conditions. Cellular profiles of 262 patients were analyzed in terms of age (age < 40 year, age 41–60 year and age > 60 year), gender, BMI (BMI < 22 kg/m2, BMI 22–25 kg/m2 and BMI > 25 kg/m2), pre-harvest haematological profile and clinical conditions (chronic disorders, group A, acute vascular group B and traumatic events, group C). A steady decline was observed in TNC and MNC counts with increasing age and BMI. In clinical conditions, group C showed a highest cellular yield followed by group A and group B respectively. Amongst the three age groups, group I (age < 40 year) showed a better cellular profiles than group II (age 41–60 year) and group III (age > 60 year). Patients with Higher TLC (>7000/μl) and platelet count (>200 × 103/μl) yielded better cellular profile in the harvest. Patient age, BMI, haematological counts and clinical condition significantly affect the bone marrow cellular profile.

Keywords

Autologous bone marrow mononuclear cell therapy Bone marrow cellular profiles Advanced medicinal therapeutic products 

References

  1. 1.
    Chernykh ER, Shevela EY, Leplina OY et al (2006) Characteristics of bone marrow cells under conditions of impaired innervations in patients with spinal trauma. Bull Exp Biol Med 141:117–120CrossRefPubMedGoogle Scholar
  2. 2.
    Lannert H, Able T, Becker S, Sommer M et al (2008) Optimizing BM harvesting from normal adults donors. Bone Marrow Transpl 42:443–447CrossRefGoogle Scholar
  3. 3.
    Buckner CD, Clift RA, Sanders JE et al (1984) Marrow harvesting from normal donors. Blood 64:630–634PubMedGoogle Scholar
  4. 4.
    Cottler-Fox M (1992) Bone marrow collection techniques. In: Areman E, Deeg H, Sacher R (eds) Bone marrow and stem cell processing: a manual of current techniques. Davis, PhiladelphiaGoogle Scholar
  5. 5.
    Sutherland DR, Anderson L, Keeney M et al (1996) The ISHAGE guidelines for CD 34+ cell determination by flow Cytometry. J Hematother 5:213–226CrossRefPubMedGoogle Scholar
  6. 6.
    Gratama JW, Keeney M, Sutherland DR (1999) Enumeration of hematopoietic stem cell and progenitor cells. Curr Protoc Cytom 6:6.4.1–6.4.22Google Scholar
  7. 7.
    Dedeepiya VD, Rao YY, Gosalakkal A et al (2012) Index of CD34+ cells and mononuclear cells in the bone marrow of spinal cord injury patients of different age groups: a comparative analysis. Bone Marrow Res 2012:1–8CrossRefGoogle Scholar
  8. 8.
    Kuranda K, Vargaftig J, de la Rochere P, Dosquet C, Charron D, Bardin F, Tonnelle C, Bonnet D, Goodhardt M (2011) Age-related changes in human hematopoietic stem/progenitor cells. Aging Cell 10(3):542–546. doi: 10.1111/j.1474-9726.2011.00675.x CrossRefPubMedGoogle Scholar
  9. 9.
    Chambers SM, Shaw CA, Gatza C, Fisk CJ et al (2007) Aging hematopoietic stem cells decline in function and exhibit epigenetic dysregulation. PLoS Biol 5:1750–1762CrossRefGoogle Scholar
  10. 10.
    Bellows CF, Zhang Y, Simmons PJ et al (2011) Influence of BMI on level of circulating progenitor cells. Obesity 19:1722–1726CrossRefPubMedPubMedCentralGoogle Scholar
  11. 11.
    Huertas Alice, Testa Ugo, Riccioni Roberta et al (2010) Bone marrow-derived progenitors are greatly reduced in patients with severe COPD and low-BMI. Respir Physiol Neurobiol 170:23–31CrossRefPubMedGoogle Scholar
  12. 12.
    Page KM, Zhang L, Mendizabal A et al (2011) Total colony forming units are a strong, independent predictor of neutrophil and platelet engraftment after unrelated umbilical cord blood transplantation: a single-center analysis of 435 cord blood transplants. Biol Blood Marrow Transpl 17:1362–1374CrossRefGoogle Scholar

Copyright information

© Indian Society of Haematology & Transfusion Medicine 2017

Authors and Affiliations

  • Deepak Pahwa
    • 1
  • Ratti Ram Sharma
    • 1
  • Neelam Marwaha
    • 1
  1. 1.Department of Transfusion MedicinePost Graduate Institute of Medical Education and ResearchChandigarhIndia

Personalised recommendations