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Community structures and genomic features of undesirable white colony-forming yeasts on fermented vegetables

  • Microbial Ecology and Environmental Microbiology
  • Published:
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Abstract

White colony-forming yeasts (WCFYs) often appear in fermented foods, depending on the storage method. Despite the ongoing research on fermented foods, the community and genome features of WCFYs have not been well studied. In this study, the community structures of WCFYs on fermented vegetables (kimchi) prepared with various raw materials were investigated using deep sequencing. Only eight operational taxonomic units (OTUs) were detected, indicating that the community structure of WCFYs on kimchi is very simple. The five most abundant OTUs represented Pichia kluyveri, Yarrowia lipolytica, Candida sake, Hanseniaspora uvarum, and Kazachstania servazzii. Using a culture-dependent method, 41 strains representing the five major OTUs were isolated from the surface of the food samples. Whole genomes of the five major yeast strains were sequenced and annotated. The total genome length for the strains ranged from 8.97 Mbp to 21.32 Mbp. This is the first study to report genome sequences of the two yeasts Pichia kluyveri and Candida sake. Genome analysis indicated that each yeast strain had core metabolic pathways such as oxidative phosphorylation; purine metabolism; glycolysis/gluconeogenesis; aminoacyl-tRNA biosynthesis; citrate cycle; but strain specific pathways were also found. In addition, no toxin or antimicrobial resistance genes were identified. Our study provides genome information for five WCFY strains that may highlight their potential beneficial or harmful metabolic effects in fermented vegetables.

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  1. These authors contributed equally to this work.

Authors and Affiliations

  1. Department of Biology, Kyung Hee University, Seoul, 02447, Republic of Korea

    Joon Yong Kim & Jin-Woo Bae

  2. Microbiology and Functionality Research Group, World Institute of Kimchi, Gwangju, 61755, Republic of Korea

    Juseok Kim, In-Tae Cha, Min Young Jung, Hye Seon Song, Yeon Bee Kim, Changsu Lee, Seung-Yeon Kang, Tae-Woon Kim & Seong Woon Roh

  3. Division of Environmental Science & Ecological Engineering, Korea University, Seoul, 02841, Republic of Korea

    Juseok Kim & Yoon-E Choi

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  1. Joon Yong Kim
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Correspondence to Tae-Woon Kim or Seong Woon Roh.

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Kim, J.Y., Kim, J., Cha, IT. et al. Community structures and genomic features of undesirable white colony-forming yeasts on fermented vegetables. J Microbiol. 57, 30–37 (2019). https://doi.org/10.1007/s12275-019-8487-y

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