Skip to main content
SpringerLink
Log in

Flavobacterium aquariorum sp. nov., isolated from freshwater of the North Han River

  • Published:
Journal of Microbiology Aims and scope Submit manuscript

Abstract

A non-motile, yellow-pigmented bacterial strain, designated IMCC34762T, was isolated from a freshwater sample collected from Lake Cheongpyeong in Korea. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain IMCC-34762T formed a lineage within the genus Flavobacterium and was most closely related to F. pectinovorum DSM 6368T (98.3% sequence similarity), followed by F. piscis CCUG 60099T (98.3%), F. branchiicola 59B-3-09T (98.2%), and F. saccharophilum DSM 1811T (98.2%). The average nucleotide identity and the genome-to-genome distance between strain IMCC34762T and the closely related strains were 61–62% and 26–27%, respectively, indicating that IMCC34762T is a novel species of the genus Flavobacterium. The major fatty acids (> 5%) of strain IMCC34762T were summed feature 3 (C16:1ω6c and/or C16:1ω7c, 17.3%), iso-C15:0 (15.0%), iso-C15:0 G (9.0%), C15:0ω6c (7.4%), iso-C15:0 (7.4%), and iso-C16:0 (5.3%). The major respiratory quinone and polyamine were MK-6 and sym-homospermidine, respectively. The major polar lipids were phosphatidylethanolamine, an unidentified aminophospholipid, and an unidentified lipid. The DNA G + C content of strain IMCC34762T was 34.4 mol%. Based on the taxonomic data presented in this study, strain IMCC-34762T represents a novel species within the genus Flavobacterium, for which the name Flavobacterium aquariorum, sp. nov. is proposed. The type strain is IMCC34762T (= KACC 19725T = NBRC 113425T).

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • Auch, A.F., von Jan, M., Klenk, H.P., and Goker, M. 2010. Digital DNA-DNA hybridization for microbial species delineation by means of genome-to-genome sequence comparison. Stand. Genomic Sci. 2, 117–134.

    Article  PubMed  PubMed Central  Google Scholar 

  • Bankevich, A., Nurk, S., Antipov, D., Gurevich, A.A., Dvorkin, M., Kulikov, A.S., Lesin, V.M., Nikolenko, S.I., Pham, S., Prjibelski, A.D., et al. 2012. SPAdes: a new genome assembly algorithm and its applications to single-cell sequencing. J. Comput. Biol. 19, 455–477.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Bergey, D.H., Harrison, F.C., Breed, R.S., Hammer, B.W., and Huntoon, F.M. 1923. Genus II. Flavobacterium gen. nov., pp. 97–117. In Bergey’s manual of determinative bacteriology, Williams & Wilkins, Baltimore, MD, USA.

    Google Scholar 

  • Bernardet, J.F. and Bowman, J.P. 2006. The genus Flavobacterium, pp. 481–531. In Dworkin, M., Falkow, S., Rosenberg, E., Schleifer, K.H., and Stackedbrandt, E. (eds.), The prokaryotes: the biology of bacteria, 3rd ed, Springer, New York, USA.

    Chapter  Google Scholar 

  • Bernardet, J.F., Nakagawa, Y., and Holmes, B. 2002. Proposed minimal standards for describing new taxa of the family Flavobacteriaceae and emended description of the family. Int. J. Syst. Evol. Microbiol. 52, 1049–1070.

    CAS  PubMed  Google Scholar 

  • Bernardet, J.F., Segers, P., Vancanneyt, M., Berthe, F., Kersters, K., and Vandamme, P. 1996. Cutting a gordian knot: Emended classification and description of the genus Flavobacterium, emended description of the family Flavobacteriaceae, and proposal of Flavobacterium hydatis nom. nov. (basonym, Cytophaga aquatilis Strohl and Tait 1978). Int. J. Syst. Evol. Microbiol. 46, 128–148.

    Google Scholar 

  • Bu, J.H. and Cha, C.J. 2018. Flavobacterium foetidum sp. nov., isolated from ginseng soil. Int. J. Syst. Evol. Microbiol. 68, 616–622.

    Article  CAS  PubMed  Google Scholar 

  • Cai, H., Zeng, Y., Wang, Y., Cui, H., and Jiang, H. 2018. Flavobacterium cyanobacteriorum sp. nov., isolated from cyanobacterial aggregates in a eutrophic lake. Int. J. Syst. Evol. Microbiol. 68, 1279–1284.

    Article  PubMed  Google Scholar 

  • Chaudhary, D.K. and Kim, J. 2017. Flavobacterium olei sp. nov., a novel psychrotolerant bacterium isolated from oil-contaminated soil. Int. J. Syst. Evol. Microbiol. 67, 2211–2218.

    Article  CAS  PubMed  Google Scholar 

  • Chaudhary, D.K. and Kim, J. 2018. Flavobacterium naphthae sp. nov., isolated from oil-contaminated soil. Int. J. Syst. Evol. Microbiol. 68, 305–309.

    Article  PubMed  Google Scholar 

  • Dahal, R.H., Chaudhary, D.K., and Kim, J. 2017. Flavobacterium flaviflagrans sp. nov., a bacterium of the family Flavobacteriaceae isolated from forest soil. Int. J. Syst. Evol. Microbiol. 67, 2653–2659.

    Article  CAS  PubMed  Google Scholar 

  • Dahal, R.H. and Kim, J. 2018. Flavobacterium ureilyticum sp. nov., a novel urea hydrolysing bacterium isolated from stream bank soil. Antonie van Leeuwenhoek 111, 2131–2139.

    Article  CAS  PubMed  Google Scholar 

  • Dong, K., Chen, F., Du, Y., and Wang, G. 2013. Flavobacterium enshiense sp. nov., isolated from soil, and emended descriptions of the genus Flavobacterium and Flavobacterium cauense, Flavobacterium saliperosum and Flavobacterium suncheonense. Int. J. Syst. Evol. Microbiol. 63, 886–892.

    Article  CAS  PubMed  Google Scholar 

  • Ekwe, A.P. and Kim, S.B. 2018. Flavobacterium commune sp. nov., isolated from freshwater and emended description of Flavobacterium seoulense. Int. J. Syst. Evol. Microbiol. 68, 93–98.

    Article  PubMed  Google Scholar 

  • Felsenstein, J. 1981. Evolutionary trees from DNA sequences: a maximum likelihood approach. J. Mol. Evol. 17, 368–376.

    Article  CAS  PubMed  Google Scholar 

  • Felsenstein, J. 1985. Confidence limits on phylogenies: an approach using the bootstrap. Evolution 39, 783–791.

    Article  Google Scholar 

  • Fitch, W.M. 1971. Toward defining the course of evolution: minimum change for a specific tree topology. Syst. Zool. 20, 406–416.

    Article  Google Scholar 

  • Fu, Y., Tang, X., Lai, Q., Zhang, C., Zhong, H., Li, W., Liu, Y., Chen, L., Sun, F., and Shao, Z. 2011. Flavobacterium beibuense sp. nov., isolated from marine sediment. Int. J. Syst. Evol. Microbiol. 61, 205–209.

    Article  CAS  PubMed  Google Scholar 

  • Fujii, D., Nagai, F., Watanabe, Y., and Shirasawa, Y. 2014. Flavobacterium longum sp. nov. and Flavobacterium urocaniciphilum sp. nov., isolated from a wastewater treatment plant, and emended descriptions of Flavobacterium caeni and Flavobacterium terrigena. Int. J. Syst. Evol. Microbiol. 64, 1488–1494.

    Article  CAS  PubMed  Google Scholar 

  • Jeon, Y.S., Lee, K., Park, S.C., Kim, B.S., Cho, Y.J., Ha, S.M., and Chun, J. 2014. EzEditor: a versatile sequence alignment editor for both rRNA- and protein-coding genes. Int. J. Syst. Evol. Microbiol. 64, 689–691.

    Article  CAS  PubMed  Google Scholar 

  • Joung, Y., Kang, H., Kim, H., Kim, T.S., Han, J.H., Kim, S.B., Ahn, T.S., and Joh, K. 2016. Flavobacterium paronense sp. nov., isolated from freshwater of an artificial vegetated island. Int. J. Syst. Evol. Microbiol. 66, 365–370.

    Article  CAS  PubMed  Google Scholar 

  • Jukes, T.H. and Cantor, C.R. 1969. Evolution of protein molecules, pp. 21–132. In Munro, H.N. (ed.), Mammalian protein metabolism. vol 3, Academic Press, New York, USA.

    Chapter  Google Scholar 

  • Kampfer, P., Busse, H.J., McInroy, J.A., and Glaeser, S.P. 2017. Flavobacterium gossypii sp. nov. isolated from the root tissue of field-grown cotton. Int. J. Syst. Evol. Microbiol. 67, 3345–3350.

    Article  CAS  PubMed  Google Scholar 

  • Kang, J.Y., Chun, J., and Jahng, K.Y. 2013. Flavobacterium aciduliphilum sp. nov., isolated from freshwater, and emended description of the genus Flavobacterium. Int. J. Syst. Evol. Microbiol. 63, 1633–1638.

    Article  CAS  PubMed  Google Scholar 

  • Khianngam, S., Akaracharanya, A., Lee, J.S., Lee, K.C., Kim, K.W., and Tanasupawat, S. 2014. Flavobacterium arsenitoxidans sp. nov., an arsenite-oxidizing bacterium from Thai soil. Antonie van Leeuwenhoek 106, 1239–1246.

    Article  CAS  PubMed  Google Scholar 

  • Kim, O.S., Cho, Y.J., Lee, K., Yoon, S.H., Kim, M., Na, H., Park, S.C., Jeon, Y.S., Lee, J.H., Yi, H., et al. 2012. Introducing EzTaxon-e: a prokaryotic 16S rRNA gene sequence database with phylotypes that represent uncultured species. Int. J. Syst. Evol. Microbiol. 62, 716–721.

    Article  CAS  PubMed  Google Scholar 

  • Kim, M., Oh, H.S., Park, S.C., and Chun, J. 2014. Towards a taxonomic coherence between average nucleotide identity and 16S rRNA gene sequence similarity for species demarcation of prokaryotes. Int. J. Syst. Evol. Microbiol. 64, 346–351.

    Article  PubMed  Google Scholar 

  • Kumar, S., Stecher, G., and Tamura, K. 2016. MEGA7: Molecular evolutionary genetics analysis version 7.0 for bigger datasets. Mol. Biol. Evol. 33, 1870–1874.

    Article  CAS  PubMed  Google Scholar 

  • Lee, Y. and Jeon, C.O. 2018. Flavobacterium alvei sp. nov., isolated from a freshwater river. Int. J. Syst. Evol. Microbiol. 68, 1919–1924.

    Article  CAS  PubMed  Google Scholar 

  • Liu, H., Lu, P., Jin, L., and Zhu, G. 2017. Flavobacterium luticocti sp. nov., isolated from wastewater. Int. J. Syst. Evol. Microbiol. 67, 369–373.

    Article  CAS  PubMed  Google Scholar 

  • Liu, Q., Siddiqi, M.Z., Liu, Q., Huq, M.A., Lee, S.Y., Choi, K.D., and Im, W.T. 2018. Flavobacterium hankyongi sp. nov., isolated from activated sludge. Int. J. Syst. Evol. Microbiol. 68, 1732–1736.

    Article  CAS  PubMed  Google Scholar 

  • Madhaiyan, M., Poonguzhali, S., Lee, J.S., Lee, K.C., and Sundaram, S. 2009. Flavobacterium glycines sp. nov., a novel facultative methylotrophic species isolated from the rhizosphere of soybean. Int. J. Syst. Evol. Microbiol. 60, 2187–2192.

    Article  CAS  PubMed  Google Scholar 

  • Meier-Kolthoff, J.P., Klenk, H.P., and Goker, M. 2014. Taxonomic use of DNA G + C content and DNA-DNA hybridization in the genomic age. Int. J. Syst. Evol. Microbiol. 64, 352–356.

    Article  CAS  PubMed  Google Scholar 

  • Minnikin, D.E., O’Donnell, A.G., Goodfellow, M., Alderson, G., Athalye, M., Schaal, A., and Parlett, J.H. 1984. An integrated procedure for the extraction of bacterial isoprenoid quinones and polar lipids. J. Microbiol. Methods 2, 233–241.

    Article  CAS  Google Scholar 

  • Moya, G., Yan, Z.F., Trinh, H., Won, K.H., Yang, J.E., Kook, M.C., and Yi, T.H. 2017. Flavobacterium hibisci sp. nov., isolated from the rhizosphere of Hibiscus syriacus L. Int. J. Syst. Evol. Microbiol. 67, 537–542.

    Article  CAS  PubMed  Google Scholar 

  • Nogi, Y., Soda, K., and Oikawa, T. 2005. Flavobacterium frigidimaris sp. nov., isolated from Antarctic seawater. Syst. Appl. Microbiol. 28, 310–315.

    Article  CAS  PubMed  Google Scholar 

  • Park, M., Nam, G.G., Kim, S., Jeon, H.T., Joung, Y., and Cho, J.C. 2017. Flavobacterium chuncheonense sp. nov. and Flavobacterium luteum sp. nov., isolated from a freshwater lake. Int. J. Syst. Evol. Microbiol. 67, 4409–4415.

    Article  CAS  PubMed  Google Scholar 

  • Park, M., Song, J., Nam, G.G., Kim, S., Joung, Y., and Cho, J.C. 2018. Flavobacterium lacicola sp. nov., isolated from a freshwater lake. Int. J. Syst. Evol. Microbiol. 68, 1565–1570.

    Article  CAS  PubMed  Google Scholar 

  • Ren, Q., Yu, M., Li, Y., Zhang, Y., Shi, X., Wu, Y., Su, Y., Wang, Y., Wang, X., and Zhang, X.H. 2018. Flavobacterium ovatum sp. nov., a marine bacterium isolated from an Antarctic intertidal sandy beach. Int. J. Syst. Evol. Microbiol. 68, 795–800.

    Article  CAS  PubMed  Google Scholar 

  • Saitou, N. and Nei, M. 1987. The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol. Biol. Evol. 4, 406–425.

    CAS  PubMed  Google Scholar 

  • Sasser, M. 1990. Identification of bacteria by gas chromatography of cellular fatty acids. MIDI Technical Note 101. MIDI Inc., Newark, DE, USA.

    Google Scholar 

  • Scherer, P. and Kneifel, H. 1983. Distribution of polyamines in methanogenic bacteria. J. Bacteriol. 154, 1315–1322.

    CAS  PubMed  PubMed Central  Google Scholar 

  • Song, L., Liu, H., Huang, Y., Dai, X., and Zhou, Y. 2013. Flavobacterium marinum sp. nov., isolated from seawater. Int. J. Syst. Evol. Microbiol. 63, 3551–3555.

    Article  CAS  PubMed  Google Scholar 

  • Stackebrandt, E., Pauker, O., Steiner, U., Schumann, P., Straubler, B., Heibei, S., and Lang, E. 2007. Taxonomic characterization of members of the genus Corallococcus: molecular divergence versus phenotypic coherency. Syst. Appl. Microbiol. 30, 109–118.

    Article  CAS  PubMed  Google Scholar 

  • Yang, F., Liu, H.M., Zhang, R., Chen, D.B., Wang, X., Yan, X., Hong, Q., and Li, S.P. 2015. Flavobacterium shanxiense sp. nov., isolated from soil. Curr. Microbiol. 70, 835–839.

    Article  CAS  PubMed  Google Scholar 

  • Yi, H. and Chun, J. 2006. Flavobacterium weaverense sp. nov. and Flavobacterium segetis sp. nov., novel psychrophiles isolated from the Antarctic. Int. J. Syst. Evol. Microbiol. 56, 1239–1244.

    Article  CAS  PubMed  Google Scholar 

  • Yoon, H.S., Aslam, Z., Song, G.C., Kim, S.W., Jeon, C.O., Chon, T.S., and Chung, Y.R. 2009. Flavobacterium sasangense sp. nov., isolated from a wastewater stream polluted with heavy metals. Int. J. Syst. Evol. Microbiol. 59, 1162–1166.

    Article  CAS  PubMed  Google Scholar 

  • Yoon, S.H., Ha, S.M., Lim, J., Kwon, S., and Chun, J. 2017. A largescale evaluation of algorithms to calculate average nucleotide identity. Antonie van Leeuwenhoek 110, 1281–1286.

    Article  CAS  PubMed  Google Scholar 

  • Yoon, J.H., Park, S., Kang, S.J., Oh, S.J., Myung, S.C., and Kim, W. 2011. Flavobacterium ponti sp. nov., isolated from seawater. Int. J. Syst. Evol. Microbiol. 61, 81–85.

    Article  CAS  PubMed  Google Scholar 

  • Zamora, L., Fernandez-Garayzabal, J.F., Sanchez-Porro, C., Palacios, M.A., Moore, E.R., Dominguez, L., Ventosa, A., and Vela, A.I. 2013. Flavobacterium plurextorum sp. nov. isolated from farmed rainbow trout (Oncorhynchus mykiss). PLoS One 8, e67741.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Zamora, L., Vela, A.I., Sanchez-Porro, C., Palacios, M.A., Moore, E.R., Dominguez, L., Ventosa, A., and Fernandez-Garayzabal, J.F. 2014. Flavobacterium tructae sp. nov. and Flavobacterium piscis sp. nov., isolated from farmed rainbow trout (Oncorhynchus mykiss). Int. J. Syst. Evol. Microbiol. 64, 392–399.

    Article  CAS  PubMed  Google Scholar 

  • Zhang, H., Cheng, M.G., Sun, B., Guo, S.H., Song, M., Li, Q., and Huang, X. 2015. Flavobacterium suzhouense sp. nov., isolated from farmland river sludge. Int. J. Syst. Evol. Microbiol. 65, 370–374.

    Article  CAS  PubMed  Google Scholar 

  • Zhang, B., Liu, Z.Q., and Zheng, Y.G. 2017. Flavobacterium quisquiliarum sp. nov., isolated from activated sludge. Int. J. Syst. Evol. Microbiol. 67, 3965–3970.

    Article  CAS  PubMed  Google Scholar 

  • Zhang, G., Xian, W., Chu, Q., Yang, J., Liu, W., Yang, L., Xiao, M., Jiang, H., and Li, W. 2016. Flavobacterium terriphilum sp. nov., isolated from soil. Int. J. Syst. Evol. Microbiol. 66, 4276–4281.

    Article  CAS  PubMed  Google Scholar 

  • Zhao, J.C., Cheng, J., Zhang, Q., Gao, Z.W., Zhang, M.Y., and Zhang, Y.X. 2018. Flavobacterium artemisiae sp. nov., isolated from the rhizosphere of Artemisia annua L. and emended descriptions of Flavobacterium compostarboris and Flavobacterium procerum. Int. J. Syst. Evol. Microbiol. 68, 1509–1513.

    Article  CAS  PubMed  Google Scholar 

  • Zhou, M.Y., Zhang, X.Y., Yang, X.D., Zhang, Y.J., He, H.L., and Ning, D. 2017. Flavobacterium ardleyense sp. nov., isolated from Antarctic soil. Int. J. Syst. Evol. Microbiol. 67, 3996–4001.

    Article  CAS  PubMed  Google Scholar 

  • Zhou, M.Y., Zhang, Y.J., Zhang, X.Y., Yang, X.D., He, H.L., Ning, D., and Du, Z. 2018. Flavobacterium phocarum sp. nov., isolated from soils of a seal habitat in Antarctica. Int. J. Syst. Evol. Microbiol. 68, 536–541.

    Article  CAS  PubMed  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Biological Sciences, Inha University, Incheon, 22212, Republic of Korea

    Yochan Joung, Hye-Jin Jang, Jaeho Song & Jang-Cheon Cho

Authors
  1. Yochan Joung
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Hye-Jin Jang
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Jaeho Song
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Jang-Cheon Cho
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Jang-Cheon Cho.

Additional information

Supplemental material for this article may be found at http://www.springerlink.com/content/120956.

Electronic supplementary material

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Joung, Y., Jang, HJ., Song, J. et al. Flavobacterium aquariorum sp. nov., isolated from freshwater of the North Han River. J Microbiol. 57, 343–349 (2019). https://doi.org/10.1007/s12275-019-8436-9

Download citation

  • Received:

  • Revised:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s12275-019-8436-9

Keywords

Search

Navigation