Journal of Microbiology

, Volume 56, Issue 4, pp 264–271 | Cite as

Identification of a novel phospholipase D gene and effects of carbon sources on its expression in Bacillus cereus ZY12

  • Yu Zhao
  • Yinfeng Xu
  • Fang Yu
  • Chunzhi Zhang
Microbial Physiology and Biochemistry


In the present study, a new strain, Bacillus cereus ZY12, producing phospholipase D (PLD) was identified. The expression of PLD in this strain was found to be induced by its substrate, phosphatidylcholine (PC), and completely silenced by other carbon sources, such as glucose, fructose, and maltose, which are generally used in microbial growth cultures, thus presenting a unique expression pattern different from other PLD-producing microorganisms. This study is the first to report on the ability of B. cereus to produce PLD, and successfully clone its PLD-coding gene and identify its function, extending the knowledge on PLD distribution and evolution in microorganisms.


Bacillus cereus phosphatidylcholine Phospholipase D substrate-induced gene expression 


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Copyright information

© The Microbiological Society of Korea and Springer Nature B.V. 2018

Authors and Affiliations

  1. 1.School of Biological EngineeringDalian Polytechnic UniversityDalianP. R. China
  2. 2.School of Food and Biological EngineeringQiqihar UniversityQiqiharP. R. China

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