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Journal of Microbiology

, Volume 55, Issue 4, pp 253–259 | Cite as

Hymenobacter daeguensis sp. nov. isolated from river water

  • Leonid N. Ten
  • Yeon-Hee Lee
  • Jae-Jin Lee
  • Su-Jin Park
  • Seung-Yeol Lee
  • Sangkyu Park
  • Dae Sung Lee
  • In-Kyu Kang
  • Hee-Young Jung
Microbial Systematics and Evolutionary Microbiology

Abstract

A Gram-stain-negative, non-motile, non-spore-forming, rod-shaped, aerobic bacterial strain, designated 16F3Y-2T, was isolated from the Han River, South Korea, and was characterized taxonomically using a polyphasic approach. Comparative 16S rRNA gene sequence analysis showed that strain 16F3Y-2T belonged to the family Cytophagaceae in the phylum Bacteroidetes and was most closely related to ‘Hymenobacter terrae’ DG7A (98.01%), H. soli PB17T (97.26%), H. glaciei VUG-A130T (96.78%), H. antarcticus VUG-A42aaT (96.72%), H. ruber PB156T (96.61%), and H. saemangeumensis GSR0100T (95.77%). The G+C content of the genomic DNA of strain 16F3Y-2T was 62.9 mol%. The isolate contained MK-7 as the predominant respiratory quinone, and summed feature 3 (C16:1 ω7c/C16:1 ω6c; 35.5%), C15:0 iso (16.9%), C16:1 ω5c (10.9%), and C15:0 anteiso (9.9%) as major fatty acids. The major polar lipid was phosphatidylethanolamine. Phenotypic and chemotaxonomic data supported the affiliation of strain 16F3Y-2T with the genus Hymenobacter. However, strain 16F3Y-2T exhibited relatively low levels of DNA-DNA relatedness with ‘H. terrae’ KCTC 32554 (44.1%) and H. soli KCTC 12607T (24.3%), clearly indicating that the isolate constitutes a new genospecies. Strain 16F3Y-2T could be differentiated from its phylogenetic neighbors on the basis of several phenotypic, genotypic, and chemotaxonomic features. Therefore, strain 16F3Y-2T represents a novel species in the genus Hymenobacter, for which the name Hymenobacter daeguensis sp. nov. is proposed. The type strain is 16F3Y-2T (=KCTC 52537T =JCM 31654T).

Keywords

Hymenobacter Bacteroidetes polyphasic taxonomy 

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Copyright information

© The Microbiological Society of Korea and Springer-Verlag Berlin Heidelberg 2017

Authors and Affiliations

  • Leonid N. Ten
    • 1
  • Yeon-Hee Lee
    • 1
  • Jae-Jin Lee
    • 1
  • Su-Jin Park
    • 1
  • Seung-Yeol Lee
    • 1
  • Sangkyu Park
    • 1
  • Dae Sung Lee
    • 2
  • In-Kyu Kang
    • 3
  • Hee-Young Jung
    • 1
    • 4
  1. 1.School of Applied BiosciencesKyungpook National UniversityDaeguRepublic of Korea
  2. 2.Department of Environmental EngineeringKyungpook National UniversityDaeguRepublic of Korea
  3. 3.Department of Horticultural ScienceKyungpook National UniversityDaeguRepublic of Korea
  4. 4.Institute of Plant MedicineKyungpook National UniversityDaeguRepublic of Korea

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