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A New record of four Penicillium species isolated from Agarum clathratum in Korea

  • Microbial Systematics and Evolutionary Microbiology
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Abstract

Agarum clathratum, brown algae, play important ecological roles in marine ecosystem, but can cause secondary environment pollution when they pile up on the beach. In order to resolve the environment problem by A. clathratum, we focus to isolate and identify Penicillium because many species are well known to produce extracellular enzymes. A total of 32 Penicillium strains were isolated from A. clathratum samples that collected from 13 sites along the mid-east coast of Korea in summer. They were identified based on morphological characters and phylogenetic analysis using β-tubulin DNA sequences as well as a combined dataset of β-tubulin and calmodulin. A total of 32 strains were isolated and they were identified to 13 Penicillium species. The commonly isolated species were Penicillium citrinum, P. roseomaculatum, and Penicillium sp. Among 13 Penicillium species, four species – P. bilaiae, P. cremeogriseum, P. madriti, and P. roseomaculatum – have not been previously recorded in Korea. For these four new species records to Korea, we provide morphological characteristics of each strain.

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References

  • Birkinshaw, J.H. and Gowlland, A. 1962. Studies in the biochemistry of micro-organisms. 110. Production and biosynthesis of orsellinic acid by Penicillium madriti G. Smith. Biochem. J. 84, 342–347.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Cho, M., Lee, D.J., Kim, J.K., and You, S. 2014. Molecular characterization and immunomodulatory activity of sulfated fucans from Agarum cribrosum. Carbohydr. Polym. 113, 507–514.

    Article  CAS  PubMed  Google Scholar 

  • Choi, C.G., Kwak, S.N., and Sohn, C.H. 2006. Community structure of subtidal marine algae at Uljin on the east coast of Korea. Algae 21, 463–470.

    Article  Google Scholar 

  • Dubrovskaya, Y.V., Sova, V., Slinkina, N., Anastyuk, S., Pivkin, M., and Zvyagintseva, T. 2012. Extracellular β-D-glucosidase of the Penicillium canescens marine fungus. Appl. Biochem. Microbiol. 48, 401–408.

    Article  CAS  Google Scholar 

  • Dutta, T., Sengupta, R., Sahoo, R., Sinha Ray, S., Bhattacharjee, A., and Ghosh, S. 2007. A novel cellulase free alkaliphilic xylanase from alkali tolerant Penicillium citrinum: production, purification and characterization. Lett. Appl. Microbiol. 44, 206–211.

    Article  CAS  PubMed  Google Scholar 

  • Edrada, R.A., Heubes, M., Brauers, G., Wray, V., Berg, A., Gräfe, U., Wohlfarth, M., Mühlbacher, J., Schaumann, K., Sudarsono, S., et al. 2002. Online analysis of xestodecalactones A–C, novel bioactive metabolites from the fungus Penicillium cf. montanense and their subsequent isolation from the sponge Xestospongia exigua. J. Nat. Prod. 65, 1598–1604.

    Article  CAS  PubMed  Google Scholar 

  • Frisvad, J.C. and Filtenborg, O. 1990. Revision of Penicillium subgenus Furcatum based on secondary metabolites and conventional characters. In Samson, R.A. and Pitt, J.I. (eds.), Advances in Penicillium and Aspergillus Systematics, Plenum Press, New York, USA.

    Google Scholar 

  • Glass, N.L. and Donaldson, G.C. 1995. Development of primer sets designed for use with the PCR to amplify conserved genes from filamentous ascomycetes. Appl. Environ. Microbiol. 61, 1323–1330.

    CAS  PubMed  PubMed Central  Google Scholar 

  • Graham, L.E., Graham, J.M., and Wilcox, L.W. 2009. Algae. 2nd ed. Pearson/Benjamin Cummings, San Francisco, California, USA.

    Google Scholar 

  • Houbraken, J., Frisvad, J.C., and Samson, R.A. 2011. Taxonomy of Penicillium section Citrina. Stud. Mycol. 70, 53–138.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Houbraken, J., Visagie, C.M., Meijer, M., Frisvad, J.C., Busby, P.E., Pitt, J.I., Seifert, K.A., Louis-Seize, G., Demirel, R., Yilmaz, N., et al. 2014. A taxonomic and phylogenetic revision of Penicillium section Aspergilloides. Stud. Mycol. 78, 373–451.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Hyde, K.D., Jones, E.B.G., Leano, E., Pointing, S.B., Poonyth, A.D., and Vrijmoed, L.L.P. 1998. Role of fungi in marine ecosystems. Biodivers. Conserv. 7, 1147–1161.

    Article  Google Scholar 

  • Jones, A.L. and Aldwinckle, H.S. 1990. Compendium of Apple and Pear Diseases. APS Press, Minnesota, USA.

    Google Scholar 

  • Jones, E.B.G. and Pang, K.L. 2012. Marine fungi and fungal-like organisms. De Gruyter, Germany.

    Book  Google Scholar 

  • Kang, J.W. 1966. On the geographical distribution of marine algae in Korea. Bull. Pusan Fish. Coll. 7, 1–125.

    Google Scholar 

  • Katoh, K. and Standley, D.M. 2013. MAFFT multiple sequence alignment software version 7: improvements in performance and usability. Mol. Biol. Evol. 30, 772–780.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Kim, H.S., Boo, S.M., Sohn, C.H., and Lee, I.K. 2013. National list of species of Korea: marine algae. National Institute of Biological Resources, Incheon, Korea.

    Google Scholar 

  • Kim, M.K., Hyun, I.H., and Kim, J.W. 2005. Identification of seedborne Penicillium spp. on Gramineae crops based on morphological characteristics. Korean J. Mycol. 33, 81–85.

    Article  Google Scholar 

  • Kim, W.G., Koo, H.M., Kim, K.H., Hyun, I.H., Hong, S.K., Cha, J.S., and Kim, D.G. 2009. List of plant diseases in Korea. Korean Society of Plant Pathology, Suwon, Korea.

    Google Scholar 

  • Kim, W.K., Sang, H.K., Woo, S.K., Park, M.S., Paul, N.C., and Yu, S.H. 2007. Six species of Penicillium associated with blue mold of grape. Mycobiology 35, 180–185.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Kohlmeyer, J. and Kohlmeyer, E. 1979. Marine Mycology. Academic Press, New York, USA.

    Google Scholar 

  • Kwaśna, H. and Nirenberg, H.I. 2005. Delimitation of Penicillium virgatum sp. nov. and P. daleae on the basis of morphological and molecular characters. Mycol. Res. 109, 974–982.

    Article  PubMed  Google Scholar 

  • Lee, S., Hong, S.B., and Kim, C.Y. 2003. Contribution to the checklist of soil-inhabiting fungi in Korea. Mycobiology 31, 9–18.

    Article  Google Scholar 

  • Li, Y., Cui, F., Liu, Z., Xu, Y., and Zhao, H. 2007. Improvement of xylanase production by Penicillium oxalicum ZH-30 using response surface methodology. Enzyme Microb. Technol. 40, 1381–1388.

    Article  CAS  Google Scholar 

  • Ma, H.G., Liu, Q., Zhu, G.L., Liu, H.S., and Zhu, W.M. 2016. Marine natural products sourced from marine-derived Penicillium fungi. J. Asian Nat. Prod. Res. 18, 92–115.

    Article  CAS  PubMed  Google Scholar 

  • McRae, C.F., Hocking, A.D., and Seppelt, R.D. 1999. Penicillium species from terrestrial habitats in the Windmill Islands, East Antarctica, including a new species, Penicillium antarcticum. Polar Biol. 21, 97–111.

    Article  Google Scholar 

  • Nicoletti, R. and Trincone, A. 2016. Bioactive compounds produced by strains of Penicillium and Talaromyces of marine origin. Mar. Drugs 14, 37.

    Article  PubMed Central  Google Scholar 

  • Panno, L., Bruno, M., Gnavi, G., and Miserere, L. 2013. Diversity, ecological role and potential biotechnological applications of marine fungi associated to the seagrass Posidonia oceanica. N. Biotechnol. 30, 685–694.

    Article  CAS  PubMed  Google Scholar 

  • Park, M.S., Eom, J.E., Fong, J.J., and Lim, Y.W. 2015. New record and enzyme activity of four species in Penicillium section Citrina from marine environments in Korea. J. Microbiol. 53, 219–225.

    Article  CAS  PubMed  Google Scholar 

  • Park, M.S., Fong, J.J., Oh, S.Y., Kwon, K.K., Sohn, J.H., and Lim, Y.W. 2014a. Marine-derived Penicillium in Korea: diversity, enzyme activity, and antifungal properties. Antonie van Leeuwenhoek 106, 331–345.

    Article  CAS  PubMed  Google Scholar 

  • Park, M.S., Lee, E.J., Fong, J.J., Sohn, J.H., and Lim, Y.W. 2014b. A new record of Penicillium antarcticum from marine environments in Korea. Mycobiology 42, 109–113.

    Article  PubMed  PubMed Central  Google Scholar 

  • Park, S.J., Min, K.J., and Park, T.G. 2012. Nutritional characteristics and screening of biological activity of A. cribrosum. Korean J. Food Nutr. 25, 842–849.

    Article  Google Scholar 

  • Passarini, M.R.Z., Santos, C., Lima, N., Berlinck, R.G.S., and Sette, L.D. 2013. Filamentous fungi from the Atlantic marine sponge Dragmacidon reticulatum. Arch. Microbiol. 195, 99–111.

    Article  CAS  PubMed  Google Scholar 

  • Peterson, S.W., Vega, F.E., Posada, F., and Nagai, C. 2005. Penicillium coffeae, a new endophytic species isolated from a coffee plant and its phylogenetic relationship to P. fellutanum, P. thiersii and P. brocae based on parsimony analysis of multilocus DNA sequences. Mycologia 97, 659–666.

    Article  CAS  PubMed  Google Scholar 

  • Pitt, J.I. 1979. The genus Penicillium and its teleomorphic states Eupenicillium and Talaromyces. Academic Press, London, UK.

    Google Scholar 

  • Rivera, K.G., Diaz, J., Chavarria-Diaz, F., Garcia, M., Urb, M., Thorn, R.G., Louis-Seize, G., Janzen, D.H., and Seifert, K.A. 2012. Penicillium mallochii and P. guanacastense, two new species isolated from Costa Rican caterpillars. Mycotaxon 119, 315–328.

    Article  Google Scholar 

  • Rivera, K.G. and Seifert, K.A. 2011. A taxonomic and phylogenetic revision of the Penicillium sclerotiorum complex. Stud. Mycol. 70, 139–158.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Rogers, S.O. and Bendich, A.J. 1994. Extraction of total cellular DNA from plants, algae and fungi. In Gelvin, S. and Schilperoort, R. (eds.), Plant molecular biology manual, Kluwer Academic, Dordrecht, Netherlands.

    Google Scholar 

  • Sabuquillo, P., De Cal, A., and Melgarejo, P. 2006. Biocontrol of tomato wilt by Penicillium oxalicum formulations in different crop conditions. Biol. Control. 37, 256–265.

    Article  Google Scholar 

  • Samson, R.A., Seifert, K.A., Kuijpers, A.F., Houbraken, J., and Frisvad, J.C. 2004. Phylogenetic analysis of Penicillium subgenus Penicillium using partial b-tubulin sequences. Stud. Mycol. 49, 175–200.

    Google Scholar 

  • Sang, H., An, T.J., Kim, C.S., Choi, Y.P., Deng, J.X., Paul, N.C., Sung, G.H., and Yu, S.H. 2013. Penicillium daejeonium sp. nov., a new species isolated from a grape and schisandra fruit in Korea. J. Microbiol. 51, 536–539.

    Article  PubMed  Google Scholar 

  • Schaumann, K. and Weide, G. 1990. Enzymatic degradation of alginate by marine fungi. Hydrobiologia 204/205, 589–596.

    Article  Google Scholar 

  • Schoch, C.L., Seifert, K.A., Huhndorf, S., Robert, V., Spouge, J.L., Levesque, C.A., Chen, W., and Fungal Barcoding Consortium. 2012. Nuclear ribosomal internal transcribed spacer (ITS) region as a universal DNA barcode marker for Fungi. Proc. Natl. Acad. Sci. USA 109, 6241–6246.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Scott, J.A., Wong, B., Summerbell, R.C., and Untereiner, W.A. 2008. A survey of Penicillium brevicompactum and P. bialowiezense from indoor environments, with commentary on the taxonomy of the P. brevicompactum group. Can. J. Microbiol. 86, 732–741.

    CAS  Google Scholar 

  • Shim, J.O., Choi, K.D., Hahn, K.D., Lee, J.H., Hyun, I.H., Lee, T.S., Kyoung, I.K., Hai, P.L., and Min, W.L. 2002. Blue mold of pear caused by Penicillium aurantiogriseum in Korea. Mycobiology 30, 105–106.

    Article  Google Scholar 

  • Smith, G. 1961. Some new and interesting species of micro-fungi II. Trans. Br. Mycol. Soc. 44, 42–50.

    Article  Google Scholar 

  • Sohn, C.H., Choi, C.G., and Kim, H.G. 2007. Algal communities and useful seaweed distribution at Gangnung and it’s vicinity in east coast of Korea. Algae 22, 45–52.

    Article  Google Scholar 

  • Sonjak, S., Uršič, V., Frisvad, J.C., and Gunde-Cimerman, N. 2007. Penicillium svalbardense, a new species from Arctic glacial ice. Antonie van Leeuwenhoek 92, 43–51.

    Article  CAS  PubMed  Google Scholar 

  • Stamatakis, A. 2006. RAxML-VI-HPC: maximum likelihoodbased phylogenetic analyses with thousands of taxa and mixed models. Bioinformatics 22, 2688–2690.

    Article  CAS  PubMed  Google Scholar 

  • Tamura, K., Peterson, D., Peterson, N., Stecher, G., Nei, M., and Kumar, S. 2011. MEGA5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. Mol. Biol. Evol. 28, 2731–2739.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Visagie, C.M., Hirooka, Y., Tanney, J.B., Whitfield, E., Mwange, K., Meijer, M., Amend, A.S., Seifert, K.A., and Samson, R.A. 2014a. Aspergillus, Penicillium and Talaromyces isolated from house dust samples collected around the world. Stud. Mycol. 78, 63–139.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Visagie, C.M., Houbraken, J., Frisvad, J.C., Hong, S.B., Klaassen, C.H.W., Perrone, G., Seifert, K.A., Varga, J., Yaguchi, T., Samson, R.A. 2014b. Identification and nomenclature of the genus Penicillium. Stud. Mycol. 78, 343–371.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Visagie, C.M., Houbraken, J., Rodriques, C., Silva Pereira, C., Dijksterhuis, J., Seifert, K.A., Jacobs, K., and Samson, R.A. 2013. Five new Penicillium species in section Sclerotiora: a tribute to the Dutch royal family. Persoonia 31, 42–62.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Visagie, C.M., Houbraken, J., Seifert, K.A., Samson, R.A., and Jacobs, K. 2015. Four new Penicillium species isolated from the fynbos biome in South Africa, including a multigene phylogeny of section Lanata-Divaricata. Mycol. Prog. 14, 96–119.

    Article  Google Scholar 

  • Wakelin, S.A., Gupta, V.V.S.R., Harvey, P.R., and Ryder, M.H. 2007. The effect of Penicillium fungi on plant growth and phosphorus mobilization in neutral to alkaline soils from southern Australia. Can. J. Microbiol. 53, 106–115.

    Article  CAS  PubMed  Google Scholar 

  • Yu, S.H. 2006. Penicillium species associated with post-harvest diseases of plant products. National Institute of Agricultural Science and Technology, Suwon, Korea.

    Google Scholar 

  • Zuccaro, A., Summerbell, R.C., Gams, W., Schroers, H.J., and Mitchell, J.I. 2004. A new Acremonium species associated with Fucus spp., and its affinity with a phylogenetically distinct marine Emericellopsis clade. Stud. Mycol. 50, 283–297.

    Google Scholar 

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Park, M.S., Lee, S. & Lim, Y.W. A New record of four Penicillium species isolated from Agarum clathratum in Korea. J Microbiol. 55, 237–246 (2017). https://doi.org/10.1007/s12275-017-6405-8

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