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Antibacterial compound produced by Pseudomonas aeruginosa strain UICC B-40, an endophytic bacterium isolated from Neesia altissima

  • Microbial Physiology and Biochemistry
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Abstract

This study’s aim was to determine the identity of antibacterial compounds produced by Pseudomonas aeruginosa strain UICC B-40 and describe the antibacterial compounds’ mechanisms of action for damaging pathogenic bacteria cells. Isolation and identification of the compounds were carried out using thin layer chromatography (TLC), nuclear magnetic resonance (NMR) spectroscopy and liquid chromatography mass spectrometry (LC-MS) analyses. Antibacterial activity was assayed via minimum inhibitory concentration (MIC) and the antibacterial compound mechanism was observed morphologically through scanning electron microscopy (SEM). This study successfully identified the (2E,5E)-phenyltetradeca-2,5-dienoate antibacterial compound (molecular weight 300 g/mol), composed of a phenolic ester, fatty acid and long chain of aliphatic group structures. MIC values for this compound were determined at 62.5 μg/ml against Staphylococcus aureus strain ATCC 25923. The mechanism of the compound involved breaking down the bacterial cell walls through the lysis process. The (2E,5E)-phenyltetradeca-2,5-dienoate compound exhibited inhibitory activity on the growth of Gram-positive bacteria.

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Authors and Affiliations

  1. Department of Biological Education, Faculty of Technics, Mathematics and Natural Sciences, University of Indraprasta PGRI, South Jakarta, 12530, Indonesia

    Rina Hidayati Pratiwi

  2. Department of Biology, Faculty of Mathematics and Natural Sciences, University of Indonesia, Depok, 16424, Indonesia

    Rina Hidayati Pratiwi & Wibowo Mangunwardoyo

  3. Microbiology Division, Research Center for Biology, Indonesian Institute of Sciences (LIPI), Jalan Raya Bogor Km 46, Cibinong, 16911, Indonesia

    Iman Hidayat

  4. Research Center for Chemistry, Indonesian Institute of Sciences (LIPI), PUSPIPTEK, Serpong, 15314, Indonesia

    Muhammad Hanafi

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  1. Rina Hidayati Pratiwi
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  2. Iman Hidayat
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  3. Muhammad Hanafi
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  4. Wibowo Mangunwardoyo
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Correspondence to Rina Hidayati Pratiwi.

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Pratiwi, R.H., Hidayat, I., Hanafi, M. et al. Antibacterial compound produced by Pseudomonas aeruginosa strain UICC B-40, an endophytic bacterium isolated from Neesia altissima . J Microbiol. 55, 289–295 (2017). https://doi.org/10.1007/s12275-017-6311-0

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  • DOI: https://doi.org/10.1007/s12275-017-6311-0

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