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Tunable photocycle kinetics of a hybrid bacteriorhodopsin/quantum dot system

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Abstract

The inclusion of inorganic nanoparticles in biological environments has led to the creation of hybrid nanosystems that are employed in a variety of applications. One such system includes quantum dots (QDs) coupled with the photoactive protein, bacteriorhodopsin (BR), which has been explored in developing enhanced photovoltaic devices. In this work, we have discovered that the kinetics of the BR photocycle can be manipulated using CdSe/CdS (core/shell) QDs. The photocycle lifetime of protein samples with varying QD amounts were monitored using time-resolved absorption spectroscopy. Concentration-dependent elongations of the bR and M state lifetimes were observed in the kinetic traces, thus suggesting that excitonic coupling occurs between BR and QDs. We propose that the pairing of BR with QDs has the potential to be utilized in protein-based computing applications, specifically for real-time holographic processors, which depend on the temporal dynamics of the bR and M photointermediates.

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Acknowledgements

Work in the laboratory of R.R.B. was supported by grants from the National Institutes of Health (GM-34548) and the Harold S. Schwenk Sr. Distinguished Chair in Chemistry. Work in the laboratory of J. Z. was partially supported by the National Science Foundation (No. CAREER-1554800). We also thank Nathan B. Gillespie for the K state spectrum shown in Fig. 5 of this report. (The K state spectrum is reproduced with permission from Ref. [24], © American Chemical Society 1999).

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  1. Department of Chemistry, University of Connecticut, 55 North Eagleville Rd, Storrs, CT, 06269-3060, USA

    Terianna J. Wax, Jordan A. Greco, Shutang Chen, Nicole L. Wagner, Jing Zhao & Robert R. Birge

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  1. Terianna J. Wax
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Correspondence to Jing Zhao or Robert R. Birge.

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Wax, T.J., Greco, J.A., Chen, S. et al. Tunable photocycle kinetics of a hybrid bacteriorhodopsin/quantum dot system. Nano Res. 12, 365–373 (2019). https://doi.org/10.1007/s12274-018-2224-4

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  • DOI: https://doi.org/10.1007/s12274-018-2224-4

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