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Imaging resolution of biocatalytic activity using nanoscale scanning electrochemical microscopy

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Abstract

Scanning electrochemical microscopy represents a powerful tool for electro(chemical) characterization of surfaces, but its applicability has been limited in most cases at microscale spatial resolution, and the greatest challenge has been the scaling down to the nanoscale for fabrication and the use of nanometer-sized tips. Here, Pt nanoelectrodes with nanometer electroactive area were fabricated and employed for imaging a distribution of gold nanoparticles (AuNPs) and bioelectrocatalytic activity of a redox-active enzyme immobilized on gold surfaces.

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Acknowledgements

J. M. A. acknowledges research funding by a “Ramon y Cajal” contract from the Spanish Ministry of Science and Innovation. A. Y. T. acknowledges a fellowship from CONICET and Fundación Carolina. We are grateful to Prof. Luis Vázquez and Tech. Andrés Valera (ICMM-CSIC) for carrying out AFM and SEM measurements, respectively. The authors also thank Dr. Elena Casero (UAM) for support in the use of SECM instrumentation and Prof. H. D. Abruña for critically reviewing this manuscript.

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Authors and Affiliations

  1. Departamento de Química Analítica y Análisis Instrumental, Universidad Autónoma de Madrid, Cantoblanco, 28049, Madrid, Spain

    José M. Abad, Emiliano Martínez-Periñán, Félix Pariente & Encarnación Lorenzo

  2. Centro de Investigación y Desarrollo en Materiales Avanzados y Almacenamiento de Energía de Jujuy-CIDMEJu (CONICET-Universidad Nacional de Jujuy), Centro de Desarrollo Tecnológico General Savio, Av. Martijena s/n, 4612, Palpalá, Jujuy, Argentina

    Alvaro Y. Tesio

  3. Instituto Madrileño de Estudios Avanzados en Nanociencia (IMDEA-Nanociencia), Campus UAM, Cantoblanco, 28049, Madrid, Spain

    Encarnación Lorenzo

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  1. José M. Abad
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  2. Alvaro Y. Tesio
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  3. Emiliano Martínez-Periñán
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  4. Félix Pariente
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  5. Encarnación Lorenzo
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Correspondence to Alvaro Y. Tesio or Encarnación Lorenzo.

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Abad, J.M., Tesio, A.Y., Martínez-Periñán, E. et al. Imaging resolution of biocatalytic activity using nanoscale scanning electrochemical microscopy. Nano Res. 11, 4232–4244 (2018). https://doi.org/10.1007/s12274-018-2011-2

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  • DOI: https://doi.org/10.1007/s12274-018-2011-2

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