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Improved production, purification and bioactivity of a polysaccharide from submerged cultured Ganoderma lucidum

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Abstract

Polysaccharides from Ganoderma lucidum showed multiple biological activities, such as immuno-modulating, antitumor, antioxidant, and hepatoprotective activity, etc. Adlay oil was added into the media to enhance polysaccharide production by submerged culture of G. lucidum in this work. The results revealed the optimal concentration of adlay oil was 1.5 % for polysaccharide production of G. lucidum. Analysis of the polysaccharide components confirmed that no novel components were biosynthesized by the addition of adlay oil. The main fraction of extracellular polysaccharide, GLEP-2, was isolated from the submerged culture broth of G. lucidum by ethanol precipitation, filtration, DEAE cellulose-52 and Sepharose CL-6B chromatography. GLEP-2, which was composed of glucose, galactose, mannose, arabinose, and rhamnose in a ratio of 332:55:32:13:3 respectively, had an average molecular weight of ~2.08 × 104 Da. The bioactivity tests demonstrated that GLEP-2 enhanced the T lymphocyte proliferation significantly at a concentration of 200 μg/mL and B lymphocyte proliferation at lower concentrations of 50 μg/mL. The results suggest polysaccharides from the submerged culture of G. lucidum are potential candidates for further development and possible commercial applications, especially in the pharmaceutical and functional foods industries.

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References

  • Bao, X., C. Liu, J. Fang, and X. Li. 2001. Structural and immunological studies of a major polysaccharide from spores of Ganoderma lucidum (Fr.) Karst. Carbohydrate Research 332: 67–74.

    Article  CAS  PubMed  Google Scholar 

  • Berovič, M., J. Habijanič, I. Zore, B. Wraber, D. Hodžar, B. Boh, and F. Pohleven. 2003. Submerged cultivation of Ganoderma lucidum biomass and immunostimulatory effects of fungal polysaccharides. Journal of Biotechnology 103: 77–86.

    Article  PubMed  Google Scholar 

  • Cao, Q.Z., and Z.B. Lin. 2006. Ganoderma lucidum polysaccharides peptide inhibits the growth of vascular endothelial cell and the induction of VEGF in human lung cancer cell. Life Sciences 78: 1457–1463.

    Article  CAS  PubMed  Google Scholar 

  • Chang, M.Y., G.J. Tsai, and J.Y. Houng. 2006. Optimization of the medium composition for the submerged culture of Ganoderma lucidum by Taguchi array design and steepest ascent method. Enyzme and Microbial Technology 38: 407–414.

    Article  CAS  Google Scholar 

  • Dong, Q., Y. Wang, L. Shi, J. Yao, J. Li, F. Ma, and K. Ding. 2012. A novel water-soluble β-d-glucan isolated from the spores of Ganoderma lucidum. Carbohydrate Polymers 353: 100–105.

    Article  CAS  Google Scholar 

  • Fang, Q.H., and J.J. Zhong. 2002a. Two-stage culture process for improved production of ganoderic acid by liquid fermentation of higher fungus Ganoderma lucidum. Biotechnology Progress 18: 51–54.

    Article  CAS  PubMed  Google Scholar 

  • Fang, Q.H., and J.J. Zhong. 2002b. Effect of initial pH on production of ganoderic acid and polysaccharide by submerged fermentation of Ganoderma lucidum. Process Biochemistry 37: 769–774.

    Article  CAS  Google Scholar 

  • Ge, Y., Y. Duan, G. Fang, and S. Wang. 2009. Polysaccharides from fruit calyx of Physalis alkekengi var. Francheti: Isolation, purification, structural features and antioxidant activities. Carbohydrate Polymers 77: 188–193.

    Article  CAS  Google Scholar 

  • Guo, L., J. Xie, Y. Ruan, L. Zhou, H. Zhu, X. Yun, Y. Jiang, L. Lü, K. Chen, Z. Min, Y. Wen, and J. Gu. 2009. Characterization and immunostimulatory activity of a polysaccharide from the spores of Ganoderma lucidum. International Immunopharmacology 9: 1175–1182.

    Article  CAS  PubMed  Google Scholar 

  • Hsieh, C., H.L. Wang, C.C. Chen, T.H. Hsu, and M.H. Tseng. 2008. Effect of plant oil and surfactant on the production of mycelial biomass and polysaccharides in submerged culture of Grifola frondosa. Biochemical Engineering Journal 38: 198–205.

    Article  CAS  Google Scholar 

  • Huang, H.C., C.I. Chen, C.N. Hung, and Y.C. Liu. 2009. Experimental analysis of the oil addition effect on mycelia and polysaccharide productions in Ganoderma lucidum submerged culture. Bioprocess and Biosystems Engineering 32: 217–224.

    Article  CAS  PubMed  Google Scholar 

  • Huang, S., J. Li, Y. Li, and Z. Wang. 2011. Purification and structural characterization of a new water-soluble neutral polysaccharide GLP-F1-1 from Ganoderma lucidum. International Journal of Biological Macromolecules 48: 165–169.

    Article  CAS  PubMed  Google Scholar 

  • Jia, J., X. Zhang, Y. Hu, Y. Wu, Q. Wang, N. Li, Q. Guo, and X. Dong. 2009. Evaluation of in vivo antioxidant activities of Ganoderma lucidum polysaccharides in STZ-diabetic rats. Food Chemistry 115: 32–36.

    Article  CAS  Google Scholar 

  • Li, Y., L. Fang, and K. Zhang. 2007. Structure and bioactivities of a galactose rich extracellular polysaccharide from submergedly cultured Ganoderma lucidum. Carbohydrate Polymers 68: 323–328.

    Article  CAS  Google Scholar 

  • Lin, S., C. Li, S. Lee, and L. Kan. 2003. Triterpene-enriched extracts from Ganoderma lucidum inhibit growth of hepatoma cells via suppressing protein kinase C, activating mitogen-activated protein kinases and G2-phase cell cycle arrest. Life Sciences 72: 2381–2390.

    Article  CAS  PubMed  Google Scholar 

  • Liu, G., and K. Zhang. 2007. Enhancement of polysaccharides production in Ganoderma lucidum by the addition of ethyl acetate extracts from Eupolyphaga sinensis and Catharsius molossus. Applied Microbiology and Biotechnology 74: 572–577.

    Article  CAS  PubMed  Google Scholar 

  • Mizuno, T., G. Wang, J. Zhang, H. Kawagishi, T. Nishitoba, J. Li, and Reishi. 1995. Ganoderma lucidum and Ganoderma tsugae: bioactive substances and medicinal effects. Food Reviews International 11: 151–166.

    Article  CAS  Google Scholar 

  • Park, J.P., S.W. Kim, H.J. Hwang, Y.J. Cho, and J.W. Yun. 2002. Stimulatory effect of plant oils and fatty acids on the exo-biopolymer production in Cordyceps militaris. Enyzme and Microbial Technology 31: 250–255.

    Article  CAS  Google Scholar 

  • Tang, Y.J., W. Zhang, and J.J. Zhong. 2009. Performance analyses of a pH-shift and DOT-shift integrated fed-batch fermentation process for the production of ganoderic acid and Ganoderma polysaccharides by medicinal mushroom Ganoderma lucidum. Bioresource technology 100: 1852–1859.

    Article  CAS  PubMed  Google Scholar 

  • Tang, Y.J., and J.J. Zhong. 2002. Fed-batch fermentation of Ganoderma lucidum for hyperproduction of polysaccharide and ganoderic acid. Enyzme and Microbial Technology 31: 20–28.

    Article  CAS  Google Scholar 

  • Tang, Y.J., and J.J. Zhong. 2003. Role of oxygen supply in submerged fermentation of Ganoderma lucidum for production of Ganoderma polysaccharide and ganoderic acid. Enyzme and Microbial Technology 32: 478–484.

    Article  CAS  Google Scholar 

  • Tsai, C., F. Yang, Z. Huang, C. Chen, Y. Yang, K. Hua, J. Li, S. Chen, and S. Wu. 2012. Oligosaccharide and peptidoglycan of Ganoderma lucidum activate the immune response in human mononuclear cells. Journal of Agriculture and Food Chemistry 60: 2830–2837.

    Article  CAS  Google Scholar 

  • Yang, F.C., Y.F. Ke, and S.S. Kuo. 2000. Effect of fatty acids on the mycelial growth and polysaccharide formation by Ganoderma lucidum in shake flask cultures. Enzyme and Microbial Technology 27: 295–301.

    Article  CAS  PubMed  Google Scholar 

  • Yang, H.L., T.X. Wu, and K.C. Zhang. 2004. Enhancement of mycelial growth and polysaccharide production in Ganoderma lucidum (the Chinese medicinal fungus, ‘Lingzhi’) by the addition of ethanol. Biotechnology Letters 26: 841–844.

    Article  CAS  PubMed  Google Scholar 

  • Ye, L., X. Zheng, J. Zhang, Q. Tang, Y. Yang, X. Wang, J. Li, F. Liu, and Y. Pan. 2011. Biochemical characterization of a proteoglycan complex from an edible mushroom Ganoderma lucidum fruiting bodies and its immunoregulatory activity. Food Research International 44: 367–372.

    Article  CAS  Google Scholar 

  • Yeh, C., H. Chen, J. Yang, W. Chuang, and F. Sheu. 2010. Polysaccharides PS-G and protein LZ-8 from Reishi (Ganoderma lucidum) exhibit diverse functions in regulating murine macrophages and T Lymphocytes. Journal of Agriculture and Food Chemistry 58: 8535–8544.

    Article  CAS  Google Scholar 

  • Zhang, J., Q. Tang, M. Zimmerman-Kordmann, W. Reutter, and H. Fan. 2002. Activation of B lymphocytes by GLIS, a bioactive proteoglycan from Ganoderma lucidum. Life Sciences 71: 623–638.

    Article  CAS  PubMed  Google Scholar 

  • Zhao, L., Y. Dong, G. Chen, and Q. Hu. 2010. Extraction, purification, characterization and antitumor activity of polysaccharides from Ganoderma lucidum. Carbohydrate Polymers 80: 783–789.

    Article  CAS  Google Scholar 

  • Zhao, W., X. Jiang, W. Deng, Y. Lai, M. Wu, and Z. Zhang. 2012. Antioxidant activities of Ganoderma lucidum polysaccharides and their role on DNA damage in mice induced by cobalt-60 gamma-irradiation. Food and Chemical Toxicology 50: 303–309.

    Article  CAS  PubMed  Google Scholar 

  • Zhu, X., A. Chen, and Z. Lin. 2007. Ganoderma lucidum polysaccharides enhance the function of immunological effector cells in immunosuppressed mice. Journal of Ethnopharmacology 111: 219–226.

    Article  CAS  PubMed  Google Scholar 

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Acknowledgments

The work was supported by the Zhejiang Provincial Natural Science Foundation of China (Project No. Y3100711) and Program for New Century Excellent Talents in University (Project No. NCET-13-1046).

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Authors and Affiliations

  1. School of Life & Environmental Sciences, Wenzhou University, Wenzhou, 325035, China

    Huabin Zhou, Furu Huang, Xiangting Wu & Hailong Yang

  2. National Engineering Laboratory for Rice and By-product Further Processing, Central South University of Forestry & Technology, Changsha, 410004, China

    Gaoqiang Liu

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  1. Huabin Zhou
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  2. Gaoqiang Liu
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  3. Furu Huang
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  4. Xiangting Wu
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  5. Hailong Yang
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Correspondence to Hailong Yang.

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Zhou, H., Liu, G., Huang, F. et al. Improved production, purification and bioactivity of a polysaccharide from submerged cultured Ganoderma lucidum . Arch. Pharm. Res. 37, 1530–1537 (2014). https://doi.org/10.1007/s12272-014-0391-8

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  • DOI: https://doi.org/10.1007/s12272-014-0391-8

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