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Analysis of the inhibitory activity of Abeliophyllum distichum leaf constituents against aldose reductase by using high-speed counter current chromatography

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Abstract

We isolated five phenolic glycosides (acteoside, eutigoside B, isoacteoside, rutin and cornoside) from Abeliophyllum distichum leaves by high-speed counter current chromatography (HSCCC) using a solvent system of ethyl acetate:n-butanol:water (8:0.7:5). We determined the purity of the 5 compounds by high-performance liquid chromatography, and confirmed their chemical structures by using nuclear magnetic resonance data. We examined the inhibitory effect of these compounds on rat lens aldose reductase. Among these compounds, acteoside (1) showed the most potent inhibitory effect, with an IC50 value of 1.39 μM. The inhibitory effect of 1 was 5.0 times greater than that of quercetin (7.05 μM), which was used as a positive control. These results suggest that acteoside may be a promising agent for the prevention or treatment of diabetic complications. Moreover, HSCCC is a promising method for the isolation and purification of biologically active compounds from natural products.

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Acknowledgments

This work was supported by Priority Research Centers Program and Basic Science Research Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Education, Science and Technology (2012R1A6A1048184 and 2012-008842).

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Authors and Affiliations

  1. Institute of Natural Medicine, Hallym University, Chuncheon, 200-702, Korea

    Hong Mei Li, Jin Kyu Kim, Jai Man Jang & Soon Sung Lim

  2. Natural Products Research Institute, Gyeonggi Institute of Science & Technology Promotion, Suwon, 443-766, Korea

    Jin Kyu Kim

  3. Agricultural College, Yanbian University, Yanji, 133-002, Jilin Prov, China

    Cheng Bi Cui

  4. Department of Food Science and Nutrition, Hallym University, Chuncheon, 200-702, Korea

    Soon Sung Lim

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  1. Hong Mei Li
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  2. Jin Kyu Kim
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Correspondence to Soon Sung Lim.

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Hong Mei Li and Jin Kyu Kim are equally contributed to this study.

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Li, H.M., Kim, J.K., Jang, J.M. et al. Analysis of the inhibitory activity of Abeliophyllum distichum leaf constituents against aldose reductase by using high-speed counter current chromatography. Arch. Pharm. Res. 36, 1104–1112 (2013). https://doi.org/10.1007/s12272-013-0127-1

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