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DNA-Extraktion aus mikrobiellen Umweltproben

  • Wissenschaft · Methoden
  • Methodenvergleich
  • Published:
BIOspektrum Aims and scope

Abstract

We compared the efficiency of ten selected DNA extraction procedures for microbes published in recent literature using four different environmental samples. The suitability was assessed based on DNA yield, visual quality, as well as PCR applicability. Four out of ten extraction procedures were found applicable to all tested environmental samples. All tested procedures were cheaper compared to commercial DNA extraction kits.

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Authors and Affiliations

  1. Institut Für Umwelt- Und Verfahrenstechnik, Lehrstuhl Biotechnologie Der Wasseraufbereitung, Btu Cottbus-Senftenberg, Cottbus, Deutschland

    Ramona Kuhn, Jörg Böllmann, Kathrin Krahl, Isaac Mbir Bryant & Marion Martienssen

  2. Institut für Umwelt- Und Verfahrenstechnik, Brandenburgische Technische Universität Cottbus-Senftenberg, Lehrstuhl Biotechnologie für Wasseraufbereitung Siemens-Halske-Ring 8, 03046, Cottbus, Deutschland

    Ramona Kuhn

Authors
  1. Ramona Kuhn
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  2. Jörg Böllmann
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  3. Kathrin Krahl
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  4. Isaac Mbir Bryant
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  5. Marion Martienssen
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Corresponding author

Correspondence to Ramona Kuhn.

Additional information

Der Lehrstuhl Biotechnologie der Wasseraufbereitung an der Brandenburgischen Technischen Universität Cottbus- Senftenberg beschäftigt sich mit der Entwicklung von Methoden und Verfahren für Reinigung industrieller Abwässer. Die Charakterisierung der mikrobiellen Gemeinschaft mittels mikrobiologischer Techniken ist dabei ein wichtiger Bereich des Lehrstuhls.

Ramona Kuhn, Jörg Böllmann, Kathrin Krahl, Isaac Mbri Bryant und Marion Martienssen (v. l. n. r.)

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Kuhn, R., Böllmann, J., Krahl, K. et al. DNA-Extraktion aus mikrobiellen Umweltproben. Biospektrum 24, 596–598 (2018). https://doi.org/10.1007/s12268-018-0963-0

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  • DOI: https://doi.org/10.1007/s12268-018-0963-0

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