, Volume 23, Issue 7, pp 833–835 | Cite as

Analyse von Präbiotika in Milch: Laktose, Lactulose und Epilactose

  • Beatrice Kuschel
  • Ines Seitl
  • Timo Stressler
  • Lutz Fischer
Biotechnologie Hochleistungsflüssigchromatographie (HPLC)


Lactulose and epilactose are potentially prebiotic isomers of the milk sugar lactose. Enzymatically they can be produced using cellobiose 2-epimerases. These sugars are notoriously hard to quantify due to their chemical similarity, which results in incomplete separation. With a new commercial stationary phase we achieved baseline separation on a basic HPLC system. The method allows the parallel quantification of lactose, epilactose and lactulose within a wide range of concentrations befitting most food samples.


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Copyright information

© Springer-Verlag GmbH Deutschland, ein Teil von Springer Nature 2017

Authors and Affiliations

  • Beatrice Kuschel
    • 1
  • Ines Seitl
    • 1
  • Timo Stressler
    • 1
  • Lutz Fischer
    • 1
  1. 1.Fachgebiet für Biotechnologie und Enzymwißsenschaft, Institut für Lebensmittelwissenschaft und BiotechnologieUniversität HohenheimStuttgartDeutschland

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