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BIOspektrum

, Volume 23, Issue 7, pp 796–797 | Cite as

Humanisierung großer Genomabschnitte mittels CRISPR und Recombineering

Anwendungen & Produkte Humanisierte Mausmodelle

Abstract

Animal models, e. g. transgenic mice, in which the endogenous gene is replaced by a human disease-associated gene variant, are essential tools for biomedical research. While CRISPR/Cas9 has successfully been used to stimulate the integration of small DNA sequences into a target locus such complex genome engineering tasks remain challenging. In this study we combined very large targeting constructs with the potential of the CRISPR/Cas9-mediated double-strand breaks to humanize a 33 kb locus in the mouse.

Literatur

  1. [1]
    Gennequin B, Otte DM, Zimmer A (2013) CRISPR/Cas-induced double-strand breaks boost the frequency of gene replacements for humanizing the mouse Cnr2 gene. Biochem Biophys Res Commun 441:815–819CrossRefPubMedGoogle Scholar

Copyright information

© Springer-Verlag GmbH Deutschland, ein Teil von Springer Nature 2017

Authors and Affiliations

  1. 1.Gene Bridges GmbHHeidelbergDeutschland

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