Humanisierung großer Genomabschnitte mittels CRISPR und Recombineering
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Animal models, e. g. transgenic mice, in which the endogenous gene is replaced by a human disease-associated gene variant, are essential tools for biomedical research. While CRISPR/Cas9 has successfully been used to stimulate the integration of small DNA sequences into a target locus such complex genome engineering tasks remain challenging. In this study we combined very large targeting constructs with the potential of the CRISPR/Cas9-mediated double-strand breaks to humanize a 33 kb locus in the mouse.