Abstract
Animal models, e. g. transgenic mice, in which the endogenous gene is replaced by a human disease-associated gene variant, are essential tools for biomedical research. While CRISPR/Cas9 has successfully been used to stimulate the integration of small DNA sequences into a target locus such complex genome engineering tasks remain challenging. In this study we combined very large targeting constructs with the potential of the CRISPR/Cas9-mediated double-strand breaks to humanize a 33 kb locus in the mouse.
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Gennequin B, Otte DM, Zimmer A (2013) CRISPR/Cas-induced double-strand breaks boost the frequency of gene replacements for humanizing the mouse Cnr2 gene. Biochem Biophys Res Commun 441:815–819
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Reiss, M., Kranz, H. Humanisierung großer Genomabschnitte mittels CRISPR und Recombineering. Biospektrum 23, 796–797 (2017). https://doi.org/10.1007/s12268-017-0875-4
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DOI: https://doi.org/10.1007/s12268-017-0875-4