In-depth structural and mechanistic analysis of biomolecular complexes is crucial for modern life sciences. Here we give an overview on hydrogendeuterium exchange mass spectrometry (HDX-MS) as an attractive tool to study the dynamic and structural features of proteins and their complexes.
This is a preview of subscription content, log in to check access
Pantazatos D, Kim JS, Klock HE et al. (2004) Rapid refinement of crystallographic protein construct definition employing enhanced hydrogen/deuterium exchange MS. Proc Natl Acad Sci USA 101:751–756CrossRefPubMedPubMedCentralGoogle Scholar
Masson GR, Jenkins ML, Burke JE (2017) An overview of hydrogen deuterium exchange mass spectrometry (HDX-MS) in drug discovery. Expert Opin Drug Discov 12:981–994CrossRefPubMedGoogle Scholar
Schaper S, Steinchen W, Krol E et al. (2017) AraC-like transcriptional activator CuxR binds c-di-GMP by a PilZ-like mechanism to regulate extracellular polysaccharide production. Proc Natl Acad Sci USA 114:E4822–E4831CrossRefPubMedPubMedCentralGoogle Scholar
Steinchen W, Schuhmacher JS, Altegoer F et al. (2015) Catalytic mechanism and allosteric regulation of an oligomeric (p)ppGpp synthetase by an alarmone. Proc Natl Acad Sci USA 112:13348–13353CrossRefPubMedPubMedCentralGoogle Scholar