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Durchflusszytometrie isolierter subzellulärer Bestandteile

Zellanalytische Methoden

  • Wissenschaft · Special: Durchflusszytometrie
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Abstract

Flow cytometry is used extensively for whole cell biology. However, subcellular components of cells, such as chromosomes, chloroplasts and mitochondria are still a challenge for each cytometrist. The small size of these particles in combination with the low number of target molecules squeeze flow cytometers to their limits. Certain techniques are already well established such as single chromosome analysis, others are still on the brink to be available for the general scientific community, e. g. the analysis of single mitochondria.

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Authors and Affiliations

  1. Helmholtz Zentrum München, Neuherberg, Germany

    Wolfgang Beisker

  2. Deutsches Forschungszentrum für Gesundheit und Umwelt (GmbH) Institut für Molekulare Toxikologie und Pharmakologie, Helmholtz Zentrum München, Ingolstädter Landstraße 1, D-85764, Neuherberg, Germany

    Wolfgang Beisker

Authors
  1. Wolfgang Beisker
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Correspondence to Wolfgang Beisker.

Additional information

Wolfgang Beisker Jahrgang 1954. 1972–1978 Physikstudium an der Universität Hannover, dort 1984 Promotion. 1984–1986 Postdoc am Lawrence Livermore National Laboratory, Livermore, CA, USA und am MD Anderson Tumor Institute, Houston, TX, USA. Seit 1987 wissenschaftlicher Mitarbeiter am Helmholtz Zentrum München im Institut für Molekulare Toxikologie und Pharmakologie, Plattform Durchflusszytometrie.

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Beisker, W. Durchflusszytometrie isolierter subzellulärer Bestandteile. Biospektrum 19, 42–44 (2013). https://doi.org/10.1007/s12268-013-0270-8

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  • DOI: https://doi.org/10.1007/s12268-013-0270-8

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