In this study, biosynthesis of silver nanoparticles (AgNPs) by using Eurotium cristatum, isolated from Fuzhuan brick-tea and its antibacterial activity has been demonstrated. AgNPs were characterized at 425 nm as maximum absorbance peak by ultraviolet-visible spectrophotometry. The images of transmission electron microscopy revealed that AgNPs are spherical shape with at 15–20 nm in size. The X-ray diffraction pattern corresponding to planes (111), (200), (220), (311), and (222) demonstrated the crystalline nature of AgNPs. Fourier transform infrared spectrum showed that functional groups involved in reduction of silver ions to metal nanoparticles. For antibacterial application, AgNPs showed antibacterial activity against Pseudomonas aeruginosa, Escherichia coli, Staphylococcus aureus, Candida albicans, and Bacillus subtilis. It also acted synergistically with various antibiotics to inhibit growth of pathogenic strains, which produced an effect greater than the sum of their individual effects. For neomycin with no resistance to C. albicans, it combined with AgNPs, which had significant synergistic effect against C. albicans, with maximum inhibitory zone at 20.9 mm, which was 2.5-fold greater than that of AgNPs alone (8.2 mm). Other antibiotics combined with AgNPs also existed similar synergistic effect. Therefore, AgNPs-synthesized by E. cristatum could enhance antibacterial activity in combination with antibiotics against pathogenic strains through synergistic effects. It might provide a new strategy for treatment of resistant bacteria.
This is a preview of subscription content, log in to check access.
Buy single article
Instant access to the full article PDF.
Price includes VAT for USA
Taylor, R., S. Coulombe, T. Otanicar, P. Phelan, A. Gunawan, W. Lv, G. Rosengarten, R. Prasher, and H. Tyagi (2013) Small particles, big impacts: a review of the diverse applications of nanofluids. J. Appl. Phys. 113: 011301.
Simi, C. K. and T. E. Abraham (2007) Hydrophobic grafted and cross-linked starch nanoparticles for drug delivery. Bioprocess Biosyst. Eng. 30: 173–180.
Veerasamy, R., T. Z. Xin, S. Gunasagaran, T. F. W. Xiang, E. F. C. Yang, N. Jeyakumar, and S. A. Dhanaraj (2011) Biosynthesis of silver nanoparticles using mangosteen leaf extract and evaluation of their antimicrobial activities. J. Saudi Chem. Soc. 15: 113–120.
Ahmed, S., M. Ahmad, B. L. Swami, and S. Ikram (2016) A review on plants extract mediated synthesis of silver nanoparticles for antimicrobial applications: a green expertise. J. Adv. Res. 7: 17–28.
Baker, S., K. M. Kumar, P. Santosh, D. Rakshith, and S. Satish (2015) Extracellular synthesis of silver nanoparticles by novel Pseudomonas veronii AS41G inhabiting Annona squamosa L. and their bactericidal activity. Spectrochim. Acta A. Mol. Biomol. Spectrosc. 136: 1434–1440.
Klaus, T., R. Joerger, E. Olsson, and C. G. Granqvist (1999) Silver-based crystalline nanoparticles, microbially fabricated. Proc. Natl. Acad. Sci. USA. 96: 13611–13614.
Sadeghi, B. and F. Gholamhoseinpoor (2015) A study on the stability and green synthesis of silver nanoparticles using Ziziphora tenuior (Zt) extract at room temperature. Spectrochim. Acta A. Mol. Biomol. Spectrosc. 134: 310–315.
Medina-Ramirez, I., S. Bashir, Z. Luo, and J. L. Liu (2009) Green synthesis and characterization of polymer-stabilized silver nanoparticles. Colloids Surf. B. Biointerfaces. 73: 185–191.
Nalawade, P., P. Mukherjee, and S. Kapoor (2014) Biosynthesis, characterization and antibacterial studies of silver nanoparticles using pods extract ofAcacia auriculiformis. Spectrochim. Acta A. Mol. Biomol. Spectrosc. 129: 121–124.
Sunkar, S. and C. V. Nachiyar (2012) Biogenesis of antibacterial silver nanoparticles using the endophytic bacterium Bacillus cereus isolated from Garcinia xanthochymus.Asian Pac. J. Trop. Biomed. 2: 953–959.
Yan, Z. F., J. Guo, Y. Yang, N. X. Liu, F. H. Tian, C. T. Li, and Y. Li (2016) Identification and physiological characteristics in cultivation of fungal strain MJAU EC021 isolated from brick tea. Microbiol. China. 43: 310–321.
Burygin, G. L., B. N. Khlebtsov., A. N. Shantrokha., L. A. Dykman., V. A. Bogatyrev., and N. G. Khlebtsov (2009) On the enhanced antibacterial activity of antibiotics mixed with gold nanoparticles. Nanoscale. Res. Lett. 4: 794–801.
Peng, Y., Z. Xiong, J. Li, J. A. Huang, C. Teng, Y. Gong, and Z. Liu (2014) Water extract of the fungi from Fuzhuan brick tea improves the beneficial function on inhibiting fat deposition. Int. J. Food Sci. Nutr. 65: 610–614.
Li, W. R., T. L. Sun, S. L. Zhou, Y. K. Ma, Q. S. Shi, X. B. Xie, and X. M. Huang (2017) A comparative analysis of antibacterial activity, dynamics, and effects of silver ions and silver nanoparticles against four bacterial strains. Int. Biodeterior. Biodegradation. 123: 304–310.
Gole, A., C. Dash, V. Ramakrishnan, S. R. Sainkar, A. B. Mandale, M. Rao, and M. Sastry (2001) Pepsin-gold colloid conjugates: preparation, characterization, and enzymatic activity. Langmuir. 17: 1674–1679.
Singh, H., J. Du, and T. H. Yi (2017) Kinneretia THG-SQI4 mediated biosynthesis of silver nanoparticles and its antimicrobial efficacy. Artif. Cells. Nanomed. Biotechnol. 45: 602–608.
Naqvi, S. Z. H., U. Kiran, M. I. Ali, A. Jamal, A. Hameed, S. Ahmed, and N. Ali (2013) Combined efficacy of biologically synthesized silver nanoparticles and different antibiotics against multidrug-resistant bacteria. Int. J. Nanomedicine. 8: 3187–3195.
Fayaz, A. M., K. Balaji, M. Girilal, R. Yadav, P. T. Kalaichelvan, and R. Venketesan (2010) Biogenic synthesis of silver nanoparticles and their synergistic effect with antibiotics: a study against grampositive and gram-negative bacteria. Nanomedicine. 6: 103–109.
Jain, J., S. Arora, J. M. Rajwade, P. Omray, S. Khandelwal, and K. M. Paknikar (2009) Silver nanoparticles in therapeutics: development of an antimicrobial gel formulation for topical use. Mol. Pharm. 6: 1388–1401.
Sathishkumar, M., K. Sneha, S. W. Won, C. W. Cho, S. Kim, and Y. S. Yun (2009) Cinnamon zeylanicum bark extract and powder mediated green synthesis of nano-crystalline silver particles and its bactericidal activity. Colloids Surf. B Biointerfaces. 73: 332–338.
Lee, W., K. J. Kim, and D. G. Lee (2014) A novel mechanism for the antibacterial effect of silver nanoparticles on Escherichia coli.Biometals. 27: 1191–1201.
Lok, C. N., C. M. Ho, R. Chen, Q. Y. He, W. Y. Yu, H. Sun, P. K. H. Tam, J. F. Chiu, and C. M. Che (2006) Proteomic analysis of the mode of antibacterial action of silver nanoparticles. J. Proteome Res. 5: 916–924.
Nomiya, K., S. Takahashi, R. Noguchi, S. Nemoto, T. Takayama, and M. Oda (2000) Synthesis and characterization of water-soluble silver(I) complexes with L-histidine (H2his) and (S)-(-)-2-pyrrolidone-5-carboxylic acid (H2pyrrld) showing a wide spectrum of effective antibacterial and antifungal activities. Crystal structures of chiral helical polymers [Ag(Hhis).n and ([Ag(Hpyrrld).2)n in the solid state. Inorg. Chem. 39: 3301–3311.
Hajipour, M. J., K. M. Fromm, A. A. Ashkarran, D. J. de Aberasturi, I. R. de Larramendi, T. Rojo, V. Serpooshan, W. J. Parak, and M. Mahmoudi (2012) Antibacterial properties of nanoparticles. Trends Biotechnol. 30: 499–511.
McShan, D., P. C. Ray, and H. Yu (2014) Molecular toxicity mechanism of nanosilver. J. Food Drug Anal. 22: 116–127.
Navarro, E., F. Piccapietra, B. Wagner, F. Marconi, R. Kaegi, N. Odzak, L. Sigg, and R. Behra (2008) Toxicity of silver nanoparticles to Chlamydomonas reinhardtii.Environ. Sci. Technol. 42: 8959–8964.
This work was financially supported by Research Program of State Key Laboratory of Food Science and Technology, Jiangnan University (NO. SKLF-ZZA-201906); Fundamental Research Funds for the Central Universities (No. JUSRP11968 and JUSRP11961).
Conflict of Interest The authors report no conflicts of interest. The authors alone are responsible for the content and writing of this article.
Ethical Statement Neither ethical approval nor informed consent was required for this study.
Publisher’s Note Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.
About this article
Cite this article
Lin, P., Wang, F., Li, C. et al. An Enhancement of Antibacterial Activity and Synergistic Effect of Biosynthesized Silver Nanoparticles by Eurotium cristatum with Various Antibiotics. Biotechnol Bioproc E 25, 450–458 (2020). https://doi.org/10.1007/s12257-019-0506-7
- silver nanoparticles
- Eurotium cristatum
- antibacterial activity
- DNA cleavage