Long noncoding RNAs (lncRNAs) play important roles in the process of cell fate determination. However, their function and expression profiles have not yet been systematically investigated during the transdifferentiation of glial precursor cells derived from dorsal root ganglia (DRG) in the peripheral nervous system. Our results demonstrated significant differences in gene architecture and expression among the three transcript types (lncRNA, mRNA, and TUCP). Distinct differences in transcript length, exon number, and ORF length were identified between lncRNAs and mRNAs after comparative analysis of their structure and sequence conservation. We found that the upregulated lncRNAs outnumbered the downregulated lncRNAs in glial precursor cells cultured with proBDNF antiserum compared with the levels in glial precursor cells cultured without proBDNF antiserum. By a series of GO and KEGG analyses, we found that the effects of some lncRNAs on their target genes in cis were related to nerve growth factor-induced cell cycle, cell phenotype change, and neuronal differentiation. The qRT-PCR verification results of lncRNAs ENSRNOT00000091991, ENSRNOT00000087717, and LNC000429 were mostly consistent with the sequencing results. The candidate lncRNAs may be associated with the neuronal transdifferentiation of glial precursor cells. Our study provides the first evidence for a remarkably diverse pattern of lncRNA expression during neuronal differentiation of glial precursor cells from rat DRG and also provides a resource for lncRNA studies in the field of cell differentiation.
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long noncoding RNA
dorsal root ganglia
long intergenic ncRNA
short hairpin RNA
poly D-lysine hydrobromide
- Tuj 1:
neuron-specific class III beta-rubulin
calcitonin gene-related peptide
the fragments per kilobase of transcript per million fragments mapped
Coding Potential Calculator
open reading frame
Kyoto Encyclopedia of Genes and Genomes
quantitative real time-polymerase chain reaction
precursor brain-derived neurotrophic factor
transcript of uncertain coding potential
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We thank Liwen Bianji, Edanz Group China (www.liwenbianji.cn/ac), for editing the English text of a draft of this manuscript.
This work was supported by the National Natural Science Foundation of China (No. 31560295, to L.Y.L.); Yunnan Applied Basic Research Projects [No. 2018FE001(-163), to L.Y.L.; 2018FE001(-016), to W.M.]; Major Scientific and Technological Achievements Cultivation Projects of Kunming Medical University (CGPY201802, to L.Y.L.); and Research Innovation Team of Yunnan Province (2019HC022).
The authors declare no conflicts of interest.
Neither ethical approval nor informed consent was required for this study.
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Dai, Y., Ma, W., Zhang, T. et al. Long Noncoding RNA Expression Profiling During the Neuronal Differentiation of Glial Precursor Cells from Rat Dorsal Root Ganglia. Biotechnol Bioproc E 25, 356–373 (2020). https://doi.org/10.1007/s12257-019-0317-x
- expression profiling
- glial precursor cell