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Production of 5-aminolevulinic Acid by Recombinant Streptomyces coelicolor Expressing hemA from Rhodobacter sphaeroides

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Abstract

Over the past two decades, intensive efforts have been made to construct recombinant Escherichia coli or Corynebacterium glutamicum by engineering C4 or C5 pathways to improve microbial production of 5-aminolevulinic acid (ALA), which has medical application for photodynamic cancer therapy and tumor diagnosis. In this study, we explored the feasibility of enhanced production of ALA by expressing C4 pathway enzyme, ALA synthase, in Streptomyces coelicolor, and medium optimization. The hemA from Rhodobacter sphaeroides was successfully integrated into the chromosome of Streptomyces coelicolor by conjugal transformation, and recombinant Streptomyces cells expressed well the foreign hemA. Glucose promoted ALA synthesis, and yeast extract showed a strong positive effect on ALA production. Optimization of casamino acid, peptone, malt extract, glycine, and succinic acid increased the product titer. In flask cultures, cell growth and ALA production of recombinant Streptomyces were 2.3 and 3.0-fold higher, respectively, in optimal medium than those of control. The maximum ALA, 137 mg/L, was obtained at 28 h in bioreactor culture, in which 3.1-fold higher cell mass and 2.9-fold greater volumetric productivity were achieved, compared to those in flask cultures.

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Acknowledgements

This work was supported by the Basic Science Research Program through the National Research foundation (NRF), funded by the Ministry of Education, Science and Technology (NRF-2012R1A1A2007214 and 2017R1D1A1B03029032).

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  1. Department of Chemical Engineering and ERI, Gyeongsang National University, Jinju, 52828, Korea

    Nu Thi Tran, Diep Ngoc Pham & Chang-Joon Kim

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  1. Nu Thi Tran
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Correspondence to Chang-Joon Kim.

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Tran, N.T., Pham, D.N. & Kim, CJ. Production of 5-aminolevulinic Acid by Recombinant Streptomyces coelicolor Expressing hemA from Rhodobacter sphaeroides. Biotechnol Bioproc E 24, 488–499 (2019). https://doi.org/10.1007/s12257-018-0484-1

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