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Expression and Clinical Significance of Translation Regulatory Long Non-Coding RNA 1 (TRERNA1) in Ependymomas

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Pathology & Oncology Research

Abstract

Long noncoding RNAs (lncRNA) have emerged as vital molecules governing epithelial-to-mesenchymal transition (EMT) in cancers. Translation regulatory RNA 1 (TRERNA1) is one such lncRNA known to enhance the transcriptional activity of the EMT-transcription factor, Snail. We have previously demonstrated differential upregulation of EMT-transcription factors and cadherin switching across various clinico-pathologic-molecular subclasses of ependymomas (EPN). With an aim to analyze the correlation between the expression of TRERNA1 in EPNs, we performed gene expression analysis for TRERNA1 on 75 Grade II/III EPNs and correlated with tumor site, C11orf95-RELA fusions, age, MIB-1 proliferative indices, and outcome wherever available. Upregulation of gene expression levels of TRERNA1 was seen in intracranial EPNs, with highest expression levels in pediatric posterior fossa EPNs. High TRERNA1 expression was found associated with higher proliferative indices (p = 0.034) and shorter progression free survival (p = 0.002). Our study, for the first time, demonstrates an association between TRERNA1 expressions and pediatric posterior fossa EPNs. Further in-vivo and in-vitro studies are required to confirm these findings and evaluate TRERNA1 as a novel biomarker and potential therapeutic target in childhood PF-EPNs.

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Acknowledgements

The authors acknowledge all consultants from the Departments of Pathology and Neurosurgery, AIIMS; all technical staff from the Neuropathology laboratory, AIIMS and Department of Biostatistics, AIIMS.

Funding

The authors are thankful to Neuro Sciences Centre, AIIMS, AIIMS Intramural Research grants, and Science and Engineering Research Board (SERB) (EMR/2016/003365) for financial support of the work. Prit Benny Malgulwar is a Senior Research Fellowship awardee (No.3/2/3/284/2014/NCD-III) from Indian Council of Medical Research (ICMR), India. Aruna Nambirajan is a Senior Research Associate (8948A/17) under the Scientists’ Pool Scheme of Council of Scientific and Industrial Research (CSIR), India.

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  1. Department of Pathology, All India Institute of Medical Sciences, Ansari Nagar, New Delhi, 110029, India

    Prit Benny Malgulwar, Aruna Nambirajan, Vaishali Suri, Chitra Sarkar & Mehar Chand Sharma

  2. Department of Neurosurgery, All India Institute of Medical Sciences, New Delhi, 110029, India

    Manmohan Singh

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  1. Prit Benny Malgulwar
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Correspondence to Mehar Chand Sharma.

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Malgulwar, P.B., Nambirajan, A., Singh, M. et al. Expression and Clinical Significance of Translation Regulatory Long Non-Coding RNA 1 (TRERNA1) in Ependymomas. Pathol. Oncol. Res. 26, 1975–1981 (2020). https://doi.org/10.1007/s12253-019-00736-8

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