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The Expression of Interferon Gamma (IFN-γ) and Interleukin 6 (IL6) in Patients with Acute Lymphoblastic Leukemia (ALL)

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Pathology & Oncology Research

Abstract

Interferon gamma (IFN-γ) and interleukin 6 (IL-6) are including the most important cytokines which have been associated with the biological behavioral and immune responses in malignancies. Based on the critical roles which these two cytokines play against tumor cells, the present study was aimed to investigate the genes expression level of IL6 and IFN-γ in patients with Acute Lymphoblastic Leukemia and compare with normal controls. Fifty-two patients with ALL and 13 healthy volunteer were under studied. The peripheral blood mononuclear cells of all patients and normal controls were separated by ficoll. The expression of interferon gamma and interleukin 6 genes were determined by RQ-PCR. Finally all data were analyzes using T student, one way ANOVA and Mann-Whitney tests were use to analyze all samples data. Our finding showed that the level of IFN-γ gene expression was significant decreased in patients with All as compared with healthy controls (83 change fold, p < 0.0001). The level of IL-6 Gene expression was not changeable in B-ALL patients as compared with healthy control (p = 0.4), but in T-ALL patients, was significantly reduced (p < 0.01). The results of present study indicated that IFN-γ gene expression reduced in ALL patients. It provides a valuable insight that immune system may disrupted in patients with ALL, which cause tumor cells escape from immune surveillance.

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Acknowledgments

Authors would like to thank the deputy research of Shahid Beheshti University of medical Science (School of Allied medical Sciences) for supporting and providing research facilities.

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Authors and Affiliations

  1. Department of Hematology and Blood Banking, School of Allied Medical Sciences, Shahid Beheshti University of Medical Sciences, Tehran, Iran

    Mehdi Allahbakhshian Farsani, Masomeh Kamel, Mahdieh Mehrpouri, Reza Shiri Heris, Mohsen Hamidpour & Mohamad Hosien Mohamadi

  2. Iran-Hemopoeitic Stem cell Research Centre (HSCRC), Shahid Beheshti University of Medical Sciences, Tehran, Iran

    Mehdi Allahbakhshian Farsani, Masomeh Kamel, Mahdieh Mehrpouri, Reza Shiri Heris, Mohsen Hamidpour & Mohamad Hosien Mohamadi

  3. Department of Internal Medicine, Taleghani General Hospital, Shahid Beheshti University of Medical Sciences, Tehran, Iran

    Sina Salari

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  1. Mehdi Allahbakhshian Farsani
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  2. Masomeh Kamel
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  7. Mohamad Hosien Mohamadi
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Correspondence to Mohsen Hamidpour.

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The author(s) declared no potential conflicts of interest with respect to the research, authorship, and/or publication of this article.

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Allahbakhshian Farsani, M., Kamel, M., Mehrpouri, M. et al. The Expression of Interferon Gamma (IFN-γ) and Interleukin 6 (IL6) in Patients with Acute Lymphoblastic Leukemia (ALL). Pathol. Oncol. Res. 26, 461–466 (2020). https://doi.org/10.1007/s12253-018-0536-z

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  • DOI: https://doi.org/10.1007/s12253-018-0536-z

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