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MicroRNA-424-5p Suppresses the Expression of SOCS6 in Pancreatic Cancer

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Pathology & Oncology Research

Abstract

MicroRNAs (miRNAs) are a group of small non-coding RNA molecules predicted to control the activity of about 30 % of all protein-coding genes in mammals. The expression of microRNA-424-5p (miR-424-5p) has been shown to vary in multiple hematological and solid organ malignancies, such as pancreatic cancer. This study aimed to characterize the function of upregulated miR-424-5p in pancreatic cancer and show how downstream suppressor of cytokine-induced signaling 6 (SOCS6) is negatively regulated by miR-424-5p. MiR-424-5p and SOCS6 expression was detected using quantitative real-time PCR (qRT-PCR) in pancreatic cancer tissues and adjacent non-tumorous ductal epithelium tissues. Luciferase reporter assays were used to assess SOCS6 as a target of miR-424-5p. The downstream effect of SOCS6 was measured by qRT-PCR after miR-424-5p inhibition and SOCS6 upregulation. The functions of miR-424-5p in vitro in pancreatic cancer cells were measured by migration and invasion assays and flow cytometry. Results suggested miR-424-5p was significantly upregulated in pancreatic cancer and suppress the expression of SOCS6, and miR-424-5p increased proliferation, migration and invasion of pancreatic cancer cells, while inhibited cell apoptosis. It was concluded that miR-424-5p is frequently upregulated in pancreatic cancer and modulates ERK1/2 signaling pathway by negatively regulating SOCS6.

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Authors and Affiliations

  1. Department of General Surgery, Xiangya Hospital, Central South University, Xiangya Road 87, 410008, Changsha, Hunan Province, People’s Republic of China

    Kemin Wu, Guohuang Hu, Peng Zhou, Jian Li, Bin He & Weijia Sun

  2. Department of Anesthesiology, Xiangya Hospital, Central South University, Changsha, China

    Xin He

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  1. Kemin Wu
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  2. Guohuang Hu
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  3. Xin He
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  4. Peng Zhou
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  5. Jian Li
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  6. Bin He
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  7. Weijia Sun
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Correspondence to Weijia Sun.

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Wu, K., Hu, G., He, X. et al. MicroRNA-424-5p Suppresses the Expression of SOCS6 in Pancreatic Cancer. Pathol. Oncol. Res. 19, 739–748 (2013). https://doi.org/10.1007/s12253-013-9637-x

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  • DOI: https://doi.org/10.1007/s12253-013-9637-x

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