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American Journal of Potato Research

, Volume 95, Issue 5, pp 564–574 | Cite as

Characterization of Late Blight Resistance Potato Breeding Lines with the RB Gene from Solanum bulbocastanum

  • Saltanat Mambetova
  • William W. Kirk
  • Noah Rosenzweig
  • David S. DouchesEmail author
Article

Abstract

Late blight, caused by Phytophthora infestans, is an important disease of potato (Solanum tuberosum). Previous work has demonstrated that the wild diploid potato species Solanum bulbocastanum showed resistance to all known genotypes of P. infestans. In this study, the RB gene (also known as Rpi-blb1) from S. bulbocastanum was transformed and expressed into conventionally bred, late blight resistant breeding lines to evaluate the effect of pyramiding late blight resistance genes. All RB potato transformation events were confirmed by polymerase chain reaction and RB transcription was detected by reverse transcriptase-polymerase chain reaction. Multiple P. infestans genotypes were used to evaluate foliar host plant resistance using a greenhouse whole plant bioassay (WPB) and field trials. The results of the WPB were dependent on different pathogen genotypes. The RB lines derived from MSM171-A late blight resistant (LBR) parental line showed less foliar late blight than other RB lines. Field trials indicated most RB lines from three LBR parental lines had lower RAUDPC values than their parent and susceptible check line. Our results suggest that stacking of R-genes may be a viable strategy to obtain late blight resistant potato varieties.

Keywords

Solanum tuberosum Phytophthora infestans Transformation Host resistance Gene pyramiding 

Resumen

El tizón tardío, causado por Phytophthora infestans, es una ernfermedad importante de la papa (Solanum tuberosum). Trabajo previo ha demostrado que la especie diploide silvestre de papa Solanum bulbocastanum mostró resistencia a todos los genotipos conocidos de P. infestans. En este estudio, el gen RB (también conocido como Rpi-blb1) de S. bulbocastanum se transformó y expresó en mejoramiento convencional, líneas de mejoramiento resistentes al tizón tardío, para evaluar el efecto de la piramidización de los genes de resistencia a la enfermedad. Se confirmaron todos los eventos de transformación de la papa RB por la reacción en cadena de la polimerasa y se detectó la transcripción de RB por la reacción en cadena de la polimerasa de reverso-transcriptasa. Se usaron múltiples genotipos de P. infestans para evaluar la resistencia foliar de la planta hospedera usando un bioensayo de planta completa en invernadero (WPB) y ensayos de campo. Los resultados de WPB dependieron de diferentes genotipos del patógeno. Las líneas RB derivadas de MSM171-A, línea parental resistente al tizón tardío (LBR), mostraron menos tizón que otras líneas RB. Los ensayos de campo indicaron que la mayoría de las líneas RB de tres líneas parentales LBR tuvieron valores más bajos de RAUDPC que sus progenitores y que el testigo susceptible. Nuestros resultados sugieren que apilando los genes R pudiera ser una estrategia viable para obtener variedades de papa resistentes al tizón tardío.

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Copyright information

© The Potato Association of America 2018

Authors and Affiliations

  1. 1.Department of Plant, Soil and Microbial SciencesMichigan State UniversityEast LansingUSA

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