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American Journal of Potato Research

, Volume 95, Issue 3, pp 301–310 | Cite as

Differential Spread of Potato virus Y (PVY) Strains O, N:O and NTN in the Field: Implications for the Rise of Recombinant PVY Strains in New Brunswick, Canada

  • Tyler D. B. MacKenzie
  • Jacques Lavoie
  • Xianzhou Nie
  • Mathuresh SinghEmail author
Article

Abstract

Potato virus Y (PVY) is a major cause of yield and quality loss in potato crops worldwide. Recently, populations of PVY strains have shifted dramatically toward recombinant strains such as PVYNTN and PVYN:O. A 2010 to 2016 survey of PVY strains in commercial fields of New Brunswick (NB), Canada, and five field trials tracking PVY spread in NB and Manitoba, were conducted to study the current status of PVY strains and their relative rates of spread. In NB, PVYO dropped from 82% of infections in 2010 to 14% in 2016, replaced mostly by PVYNTN (64%) and PVYN:O (22%).In field trials with Russet Burbank and Gold rush varieties, PVYNTN spread most effectively compared to PVYN:O and PVYO. Strain-specific PVY spread varied with the potato variety, possibly due to selective PVYO resistance in Goldrush, mostly expressed at the plant-to-plant transmission level with little difference in transduction to tubers in infected plants. Relevance of in-field differences in spread of strains to changes in regional PVY populations, and potential mechanisms responsible, are discussed.

Keywords

PVYO PVYN:O PVYNTN Virus spread PVY resistance 

Resumen

El virus Y de la papa (PVY) es una causa importante de pérdida de rendimiento y calidad en los cultivos de papa en el mundo. Recientemente, las poblaciones de las variantes de PVY han girado dramáticamente hacia recombinantes, tales como el PVYNTN y PVYN:O. Se condujo un estudio de 2010 a 2016 de las variantes de PVY en campos comerciales de New Brunswick (NB), Canada, y cinco ensayos de campo, siguiendo la huella de la dispersión en NB y Manitoba, para estudiar el estatus actual de las variantes del PVY y sus niveles relativos de dispersión. En NB, PVYO bajó de 82% de infecciones en 2010 a 14% en 2016, reemplazado mayormente por PVYNTN (64%) y PVYN:O (22%). En ensayos de campo con las variedades Russet Burbank y Goldrush, el PVYNTN se dispersó más efectivamente comparado con PVYN:O y PVYO. La dispersión específica por variante varío con la variedad de papa, posiblemente debido a la resistencia selectiva al PVYO en Goldrush, mayormente expresada en el nivel de la transmisión de planta a planta, con poca diferencia en la transducción a tubérculos en plantas infectadas. Se discuten la relevancia de las diferencias en el campo en la dispersión de las variantes a cambios en las poblaciones regionales de PVY, y mecanismos potenciales responsables.

Notes

Acknowledgements

The authors thank the Agriculture and Agri-Food Canada and Canadian Horticultural Council – Science cluster, Growing Forward 2 and the New Brunswick Embracing Innovation in Agriculture and Agri-Food Program for funding this research. We also thank the many staff members of ACS Inc., Gaia Consulting Ltd. (Manitoba) and the New Brunswick Department of Agriculture, Aquaculture and Fisheries for their help, particularly with the large amount of field work and maintenance required for these field trials. We also thank Dr. Jari Valkonen for helpful consultations during the manuscript revision process.

References

  1. Basky, Z., and A. Almasi. 2005. Differences in aphid transmissibility and translocation between PVYN and PVYO isolates. Journal of Pest Science 78: 67–75.CrossRefGoogle Scholar
  2. Beemster, A.B.R. 1976. Translocation of the potato viruses YN and YO in some potato varieties. Potato Research 19: 169–172.Google Scholar
  3. Carroll, J.E., D.M. Smith, and S.M. Gray. 2016. Preferential acquisition and inoculation of PVYNTN over PVYO in potato by the green peach aphid Myzus persicae (Sulzer). Journal of General Virology 97: 797–802.Google Scholar
  4. Chikh-Ali, M., T. Maoka, K.T. Natsuaki, and T. Natsuaki. 2010. The simultaneous differentiation of Potato virus Y strains including the newly described strain PVYNTN-NW by multiplex PCR assay. Journal of Virological Methods 165: 15–20.CrossRefPubMedGoogle Scholar
  5. Chikh-Ali, M., S.M. Gray, and A.V. Karasev. 2013. An improved multiplex IC-RT-PCR assay distinguishes nine strains of Potato virus Y. Plant Disease 97: 1370–1374.CrossRefGoogle Scholar
  6. Chikh-Ali, M., N.A. Bosque-Pérez, D. Vander Pol, D. Sembel, and A.V. Karasev. 2016. Occurrence and molecular characterization of recombinant Potato virus YNTN isolates from Sulawesi, Indonesia. Plant disease 100: 269–275.CrossRefGoogle Scholar
  7. Crosslin, J.M., P.B. Hamm, D.C. Hane, J. Jaeger, C.R. Brown, P.J. Shiel, P.H. Berger, and R.E. Thornton. 2006. The occurrence of PVYO, PVYN, and PVYN:O Strains of Potato virus Y in certified potato seed lot trials in Washington and Oregon. Plant Disease 90: 1102–1105.Google Scholar
  8. Davie, K., R. Holmes, J. Pickup, and C. Lacomme. 2017. Dynamics of PVY strains in field grown potato: Impact of strain competition and ability to overcome host resistance mechanisms. Virus Research.  https://doi.org/10.1016/j.virusres.2017.06.012.
  9. Djilani-Khouadja, L., M. Glais, C. Kerlan Tribodet, and H. Fakhfakh. 2010. Incidence of potato viruses and characterisation of Potato virus Y variability in late season planted potato crops in northern Tunisia. European Journal of Plant Pathology 126: 479–488.CrossRefGoogle Scholar
  10. Fageria, M.S., M. Singh, U. Nanayakkara, Y. Pelletier, X. Nie, and D. Wattie. 2013. Monitoring current season spread of Potato virus Y in potato fields using ELISA and real-time RT-PCR. Plant Disease 97: 641–644.CrossRefGoogle Scholar
  11. Fereres, A., and A. Moreno. 2009. Behavioural aspects influencing plant virus transmission by homopteran insects. Virus Research. 141: 158–168.CrossRefPubMedGoogle Scholar
  12. Fox, A., L.E. Collins, R. Macarthur, L.F. Blackburn, and P. Northing. 2017. New aphid vectors and efficiency of transmission of Potato virus A and strains of Potato virus Y in the UK. Plant Pathology 66: 325–335.CrossRefGoogle Scholar
  13. Funke, C.N., O.V. Nikolaeva, K.J. Green, L.T. Tran, M. Chikh-Ali, A. Quintero-Ferrer, R.A. Cating, K.E. Frost, P.B. Hamm, N. Olsen, M.J. Pavek, S.M. Gray, J.M. Crosslin, and A.V. Karasev. 2017. Strain-specific resistance to Potato virus Y (PVY) in potato and its effect on the relative abundance of PVY strains in commercial potato fields. Plant Disease 101: 20–28.CrossRefGoogle Scholar
  14. Gray, S., S. De Boer, J. Lorenzen, A. Karasev, J. Whitworth, P. Nolte, R.P. Singh, A. Boucher, and H. Xu. 2010. Potato virus Y: An evolving concern for potato crops in the United States and Canada. Plant Disease 94: 1384–1397.CrossRefGoogle Scholar
  15. Green, K.J., C.J. Brown, S.M. Gray, and A.V. Karasev. 2017. Phylogenetic study of recombinant strains of Potato virus Y. Virology 507: 40–52.CrossRefPubMedGoogle Scholar
  16. Gugerli, P., and W. Gehriger. 1980. Enzyme-linked immunosorbent assay (ELISA) for the detection of potato leafroll virus and potato virus Y in potato tubers after artificial break of dormancy. Potato Research 23: 353–359.CrossRefGoogle Scholar
  17. Karasev, A.V., and S.M. Gray. 2013. Continuous and emerging challenges of Potato virus Y in potato. Annual Review of Phytopathology 51: 571–586.CrossRefPubMedGoogle Scholar
  18. Kehoe, M.A., and R.A.C. Jones. 2016. Improving Potato virus Y strain nomenclature: Lessons from comparing isolates obtained over a 73-year period. Plant Pathology 65: 322–333.CrossRefGoogle Scholar
  19. Lorenzen, J.H., L.M. Piche, N.C. Gudmestad, T. Meacham, and P. Shiel. 2006. A multiplex PCR assay to characterize Potato virus Y isolates and identify strain mixtures. Plant Disease 90: 935–940.CrossRefGoogle Scholar
  20. MacKenzie, T.D.B., M.S. Fageria, X. Nie, and M. Singh. 2014. Effects of crop management practices on current-season spread of Potato Virus Y. Plant Disease 98: 213–222.Google Scholar
  21. MacKenzie, T.D., X. Nie, and M. Singh. 2016. Crop management practices and reduction of on-farm spread of Potato virus Y: A 5-year study in commercial potato fields in New Brunswick, Canada. American Journal of Potato Research 93: 552–563.Google Scholar
  22. MacKenzie, T.D., J. Lavoie, X. Nie, and M. Singh. 2017. Effectiveness of combined use of mineral oil and insecticide spray in reducing Potato Virus Y (PVY) spread under field conditions in New Brunswick, Canada. American Journal of Potato Research 94: 70–80.Google Scholar
  23. Mello, A.F.S., R.A. Olarte, S.M. Gray, and K.L. Perry. 2011. Transmission efficiency of Potato virus Y strains PVYO and PVYN-Wi by five aphid species. Plant Disease 95: 1279–1283.CrossRefGoogle Scholar
  24. Mondal, S., and S.M. Gray. 2017. Sequential acquisition of Potato virus Y strains by Myzus persicae favors the transmission of the emerging recombinant strains. Virus Research.  https://doi.org/10.1016/j.virusres.2017.06.023.
  25. Mondal, S., E.J. Wenninger, P.J. Hutchinson, J.L. Whitworth, D. Shrestha, S.D. Eigenbrode, and N.A. Bosque-Pérez. 2016. Comparison of transmission efficiency of various isolates of Potato virus Y among three aphid vectors. Entomologia Experimentaliset Applicata 158: 258–268.CrossRefGoogle Scholar
  26. Nanayakkara, U.N., M. Singh, Y. Pelletier, and X. Nie. 2012. Investigation of Potato virus Y strain status and variant population in potatoes in New Brunswick, Canada. American Journal of Potato Research 89: 232–239.Google Scholar
  27. Nie, X., and R.P. Singh. 2001. A novel usage of random primers for multiplex RT-PCR detection of virus and viroid in aphids, leaves, and tubers. Journal of Virological Methods 91: 37–49.CrossRefPubMedGoogle Scholar
  28. Nie, X., and R.P. Singh. 2003. Evolution of north American PVYNTN strain Tu 660 from local PVYN by mutation rather than recombination. Virus Genes 26: 39–47.CrossRefPubMedGoogle Scholar
  29. Nie, B., M. Singh, A. Murphy, A. Sullivan, C. Xie, and X. Nie. 2012. Response of potato cultivars to five isolates belonging to four strains of Potato virus Y. Plant Disease 96: 1422–1429.CrossRefGoogle Scholar
  30. Pelletier, Y., X. Nie, M. Giguere, U. Nanayakkara, E. Maw, and R. Foottit. 2012. A new approach for the identification of aphid vectors (Hemiptera: Aphididae) of Potato Virus Y. Journal of Economic Entomology 105: 1909–1914.CrossRefPubMedGoogle Scholar
  31. R Core Team. 2017. R: A language and environment for statistical computing. R Foundation for Statistical Computing, Vienna, Austria. http://www.R-project.org.
  32. Rowley, J.S., S.M. Gray, and A.V. Karasev. 2015. Screening potato cultivars for new sources of resistanceto Potato virus Y. American Journal of Potato Research 92: 38–48.CrossRefGoogle Scholar
  33. Schubert, J., V. Fomitcheva, and J. Sztangret-Wiśniewska. 2007. Differentiation of Potato virus Y strains using improved sets of diagnostic PCR-primers. Journal of Virological Methods 140: 66–74.CrossRefPubMedGoogle Scholar
  34. Singh, R.P. 1992. Incidence of the tobacco veinal necrotic strain of Potato virus Y (PVYN) in Canada in 1990 and 1991 and scientific basis for eradication of the disease. Canadian Plant Disease Survey 72: 113–119.Google Scholar
  35. Singh, R.P., D.L. McLaren, X. Nie, and M. Singh. 2003. Possible escape of a recombinant isolate of Potato virus Y by serological indexing and methods of its detection. Plant Disease 87: 679–685.CrossRefGoogle Scholar
  36. Srinivasan, R., D.G. Hall, F.A. Cervantes, J.M. Alvarez, and J.L. Whitworth. 2012. Strain specificity and simultaneous transmission of closely related strains of a Potyvirus by Myzus persicae. Journal of Economic Entomology 105: 783–791.Google Scholar
  37. Syller, J., and A. Grupa. 2016. Antagonistic within-host interactions between plant viruses: Molecular basis and impact on viral and host fitness. Molecular Plant Pathology 17: 769–782.CrossRefPubMedGoogle Scholar
  38. Tian, Y.P., and J. Valkonen. 2015. Recombination of strain O segments to HCpro-encoding sequence of strain N of Potato virus Y modulates necrosis induced in tobacco and in potatoes carrying resistance genes Ny or Nc. Molecular Plant Pathology 16: 735–747.CrossRefPubMedGoogle Scholar
  39. Verbeek M., P.G.M. Piron, A.M. Dullemans, C. Cuperus and R.A.A. van der Vlugt. 2010. Determination of aphid transmission efficiencies for N, NTN and Wilga strains of Potato virus Y. Annals of Applied Biology 156:39–49.Google Scholar
  40. Wróbel, S. 2009. The retention of PVY in the stylet of Myzus persicae Sulz. After the application of mineral oil on potato plants. Plant breeding and seed. Science 60: 3–12.Google Scholar

Copyright information

© The Potato Association of America 2018

Authors and Affiliations

  • Tyler D. B. MacKenzie
    • 1
  • Jacques Lavoie
    • 2
  • Xianzhou Nie
    • 3
  • Mathuresh Singh
    • 1
    Email author
  1. 1.Agricultural Certification Services Inc.FrederictonCanada
  2. 2.New Brunswick Department of Agriculture, Aquaculture and Fisheries, Potato Development CentreWicklowCanada
  3. 3.Potato Research Centre, Agriculture and Agri-Food CanadaFrederictonCanada

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