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Folia Microbiologica

, Volume 63, Issue 3, pp 325–333 | Cite as

Chloramine T induced oxidative stress and the response of antioxidant system in Phanerochaete chrysosporium

  • Burcu Tongul
  • Berna Kavakcıoğlu
  • Leman Tarhan
Original Article
  • 65 Downloads

Abstract

In this study, the effect of chloramine T (Chl-T) on the activities of superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GSH-Px), glutathione reductase (GR) and glutathione S-transferase (GST); the levels of reduced (GSH) and oxidised glutathione (GSSG) and their ratios; and also membrane lipid peroxidation (LPO) levels in Phanerochaete chrysosporium were investigated in a dose- (0.25–1 mmol/L) and time-dependent (1.5–9 h) manner. The highest SOD activity was observed in 0.5 mmol/L Chl-T at 6th hour as 1.48-fold of its control. The observed highest level in CAT activities was 4.6-fold of control in 0.5 and 0.75 mmol/L at the 6th hour. The GSH levels that were over the control showed decreasing tendency from the beginning of incubation, except 0.25 mmol/L. In contrast with GSH level variations, GSSG levels reached 10.0-fold of its control by showing increasing tendency with the increases in concentration and time. While the GSH/GSSG ratios were over the control at 0.25 mmol/L during all incubation, they fell under the control values at the earlier hours of incubation with the increasing concentrations of Chl-T. Glutathione-related enzymes GSH-Px, GR and GST were also induced with Chl-T treatment, and the highest activities were 3.29-, 7.5- and 6.56-fold of their controls, respectively. On the other hand, the increases in LPO levels with increasing concentration and time up to 5.27-fold of its control showed that the inductions observed in antioxidant system could not prevent the Chl-T-based oxidative stress.

Keywords

Antioxidant enzymes Chloramine T Lipid peroxidation Phanerochaete chrysosporium Reduced-oxidised glutathione 

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Copyright information

© Institute of Microbiology, Academy of Sciences of the Czech Republic, v.v.i. 2017

Authors and Affiliations

  1. 1.The Graduate School of Natural and Applied SciencesUniversity of Dokuz EylulIzmirTurkey
  2. 2.Faculty of Science, Department of ChemistryUniversity of Dokuz EylulIzmirTurkey

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