Folia Microbiologica

, Volume 63, Issue 3, pp 307–314 | Cite as

Clinical strains of Lactobacillus reduce the filamentation of Candida albicans and protect Galleria mellonella against experimental candidiasis

  • Rodnei Dennis Rossoni
  • Marisol dos Santos Velloso
  • Lívia Mara Alves Figueiredo
  • Carolina Pistille Martins
  • Antonio Olavo Cardoso Jorge
  • Juliana Campos Junqueira
Original Article

Abstract

Candida albicans is the most common human fungal pathogen and can grow as yeast or filaments, depending on the environmental conditions. The filamentous form is of particular interest because it can play a direct role in adherence and pathogenicity. Therefore, the purpose of this study was to evaluate the effects of three clinical strains of Lactobacillus on C. albicans filamentation as well as their probiotic potential in pathogen-host interactions via an experimental candidiasis model study in Galleria mellonella. We used the reference strain Candida albicans ATCC 18804 and three clinical strains of Lactobacillus: L. rhamnosus strain 5.2, L. paracasei strain 20.3, and L. fermentum strain 20.4. First, the capacity of C. albicans to form hyphae was tested in vitro through association with the Lactobacillus strains. After that, we verified the ability of these strains to attenuate experimental candidiasis in a Galleria mellonella model through a survival curve assay. Regarding the filamentation assay, a significant reduction in hyphae formation of up to 57% was observed when C. albicans was incubated in the presence of the Lactobacillus strains, compared to a control group composed of only C. albicans. In addition, when the larvae were pretreated with Lactobacillus spp. prior to C. albicans infection, the survival rate of G. mellonela increased in all experimental groups. We concluded that Lactobacillus influences the growth and expression C. albicans virulence factors, which may interfere with the pathogenicity of these microorganisms.

Notes

Compliance with ethical standards

This study has been approved by the Ethics Committee of São Paulo State University (Unesp) under protocol 560.479.

Conflict of interest

The authors declare that they have no conflict of interest.

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Copyright information

© Institute of Microbiology, Academy of Sciences of the Czech Republic, v.v.i. 2017

Authors and Affiliations

  • Rodnei Dennis Rossoni
    • 1
  • Marisol dos Santos Velloso
    • 1
  • Lívia Mara Alves Figueiredo
    • 1
  • Carolina Pistille Martins
    • 1
  • Antonio Olavo Cardoso Jorge
    • 1
  • Juliana Campos Junqueira
    • 1
  1. 1.Department of Biosciences and Oral DiagnosisSão Paulo State University (Unesp), Institute of Science and TechnologySão José dos CamposBrazil

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