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Folia Microbiologica

, Volume 63, Issue 3, pp 283–290 | Cite as

Effect of hyperbaric air on endotoxin from Bacteroides fragilis strains

  • Dittmar Chmelař
  • Adéla Kašíková
  • Petrana Martineková
  • Michal Hájek
  • Miroslav Rozložník
  • Marek Brabec
  • Jana Janečková
  • Jana Vobejdová
  • Ivan Čižnár
Original Article

Abstract

The aim of the project was to determine any effect of hyperbaric air on Bacteroides fragilis strains cultivated under hyperbaric conditions. Previously, it was hypothesized that there was a correlation between the presence of Bacteroides bacteria in patients preferring a meaty diet and cancer of the small intestine, and particularly of the large intestine and rectum. With respect to the fact that Bacteroides fragilis (BAFR) group are important producers of endotoxins, measurement and statistical evaluation of endotoxin production by individual strains of isolated Bacteroides species were used to compare bacteria isolated from various clinical samples from patients with colon and rectum cancer in comparison with strains isolated from other non-cancer diagnoses. Endotoxin production was proven by quantitative detection using the limulus amebocyte lysate (LAL) test in EU/mL. Production of endotoxins in these bacteria cultured under hyperbaric air conditions was higher than those strains cultured under normobaric anaerobic conditions. But these differences in endotoxin production were not statistically significant (t test with log-transformed data, p value = 0.0910). Based on a two-tier t test for lognormal data, it is possible to cautiously conclude that a statistically significant difference was found between endotoxin production by Bacteroides fragilis strains isolated from non-carcinoma diagnoses (strains (1–6) and strains isolated from colorectal carcinoma diagnoses (strains 7–8; Wilcoxon non-parametric test p = 0.0132; t test = 0.1110; t test with log-transformed data, p value = 0.0294).

Notes

Acknowledgments

This work could be accomplished thanks to the financial contribution granted for the SGS01/LF/2016 project of the specific university research of the Faculty of Medicine, University of Ostrava.

Authors’ contributions

DCh: the author of the article and scenario of experiments and article writing; AK, PM: organization of experimental work in Faculty of Medicine of University of Ostrava; MH, IČ: suggestions and checking the manuscript.

Compliance with ethical standards

Additional informed consent was obtained from all individual patients included in the study article.

Conflict of interest

The authors declare that they have no conflict of interest.

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Copyright information

© Institute of Microbiology, Academy of Sciences of the Czech Republic, v.v.i. 2017

Authors and Affiliations

  • Dittmar Chmelař
    • 1
    • 2
  • Adéla Kašíková
    • 1
  • Petrana Martineková
    • 1
  • Michal Hájek
    • 1
    • 3
  • Miroslav Rozložník
    • 1
  • Marek Brabec
    • 4
    • 5
  • Jana Janečková
    • 6
  • Jana Vobejdová
    • 6
  • Ivan Čižnár
    • 1
    • 7
  1. 1.Department of Biomedical Sciences, Institute of Microbiology and Immunology, Faculty of MedicineUniversity of OstravaOstrava-ZabrehCzech Republic
  2. 2.Czech Anaerobic Bacteria Reference Laboratory, Faculty of MedicineUniversity of OstravaOstrava-ZabrehCzech Republic
  3. 3.Centre of Hyperbaric MedicineOstrava City HospitalOstravaCzech Republic
  4. 4.Department of Nonlinear Modelling, Institute of Computer ScienceAcademy of Science of the Czech RepublicPragueCzech Republic
  5. 5.National Institute of Public HealthPragueCzech Republic
  6. 6.Department of Infection DiagnosticLitomyšl Town Hospital of Pardubice RegionLitomyšlCzech Republic
  7. 7.Faculty of Public HealthSlovak Medical UniversityBratislavaSlovak Republic

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