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Folia Microbiologica

, Volume 63, Issue 3, pp 353–359 | Cite as

Molecular identification of Leishmania spp. isolates causes cutaneous leishmaniasis (CL) in Sanliurfa Province, Turkey, where CL is highly endemic

  • Gulcan Gurses
  • Mehmet Ozaslan
  • Fadile Yıldız Zeyrek
  • Ibrahim H. Kılıç
  • Nebiye Yentür Doni
  • I. Didem Karagöz
  • Nermin Uluca
Original Article
  • 208 Downloads

Abstract

Cutaneous leishmaniasis (CL) is an important public health problem in Turkey. CL has been most frequently seen in Sanliurfa. There is an expectation of increase in the population of leishmaniasis cases with the influence of Syrian refugees arriving in Turkey. In this study we aimed to diagnosis of CL and identifying of parasite from Leishmania isolates by using ITS 1 PCR RFLP. Samples were collected from 135 CL patients in Sanliurfa. After the specimens were inoculated in medium NNN, the ones which were cultures positive were cultivated in RPMI 1640 followed by PCR-RFLP. Genomic DNA was extracted phenol-chloroform procedure. Samples were examined by using ITS 1 PCR followed by RFLP analysis. Our results indicated that two species, L. tropica (132 samples) and L. major (3 samples), are responsible for cutaneous leishmaniasis in Sanlıurfa. Our study is the first scientific study in which it is reported molecular analyses of cutaneous leishmaniasis cases caused by L. major in Sanliurfa in Southestern Anatolia Region. Because CL cases caused by L.major are detected in our study, it is considered that genotyping is important for diagnosis of Leishmania and following change of epidemiology.

Notes

Funding information

This study was supported by Gaziantep University Scientific Research Fund with project number FED.13.16.

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

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Copyright information

© Institute of Microbiology, Academy of Sciences of the Czech Republic, v.v.i. 2017

Authors and Affiliations

  • Gulcan Gurses
    • 1
  • Mehmet Ozaslan
    • 2
  • Fadile Yıldız Zeyrek
    • 3
  • Ibrahim H. Kılıç
    • 2
  • Nebiye Yentür Doni
    • 1
  • I. Didem Karagöz
    • 2
  • Nermin Uluca
    • 3
  1. 1.Vocational School of Health ServiceHarran UniversitySanliurfaTurkey
  2. 2.Department of BiologyGaziantep UniversityGaziantepTurkey
  3. 3.Department of Clinical MicrobiologyHarran UniversitySanliurfaTurkey

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