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Electrospun Skin Tissue Engineering Scaffolds Based on Polycaprolactone/Hyaluronic Acid/L-ascorbic Acid

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Abstract

Skin tissue engineering is an evolving method to reconstruct skin damages caused by disease, burn or trauma. In skin tissue engineering, scaffolds should prepare three-dimensional structure for skin cells. Electrospinning technique has been widely applied for producing nano/micro-scale fiber scaffolds in tissue engineering. In this study, electrospun scaffolds based on polycaprolactone (PCL) and hyaluronic acid (HA) containing L-ascorbic acid (AA) were fabricated. Morphology, contact angle, functional groups, biodegradability and drug release of the scaffolds were evaluated. L929 fibroblast cells seeded on nanofibrous scaffolds and cell attachment and viability were evaluated as well. According to the results, the fibers diameter were less than 180 nm and by adding hyaluronic acid, the hydrophilicity of scaffolds increased and degradation rate was adjusted. The encapsulation efficiency and successful release of ascorbic acid in nanofibrous scaffolds were demonstrated. According to the results, the cell growth, proliferation and adhesion of L929 fibroblast cells on the PCL/HA/AA scaffolds were enhanced in comparison with the PCL scaffold. Moreover, PCL/HA (80:20) containing 40 mg of AA nanofibrous scaffold could be potentially applied for skin tissue engineering.

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References

  1. M. H. Kailani, H. Jafar, and A. Awidi, Skin Tissue Eng. Regen. Med., 163 (2016).

  2. N. Bhardwaj, D. Chouhan, and B. B. Mandal, “Functional 3D Tissue Engineering Scaffolds”, pp.345–365, Woodhead Publishing, 2018.

  3. M. Janmohammadi and M. S. Nourbakhsh, Int. J. Polym. Mater. Polym. Biomater., 68, 527 (2018).

    Article  CAS  Google Scholar 

  4. K. Ghosal, A. Chandra, G. Praveen, S. Snigdha, S. Roy, C. Agatemor, S. Thomas, and I. Provaznik, Sci. Rep., 8, 1 (2018).

    Article  CAS  Google Scholar 

  5. M. M. Hasan, A. M. Alam, and K. A. Nayem, Eur. Sci. J., 10, 265 (2014).

    Google Scholar 

  6. N. Bhardwaj and S. C. Kundu, Biotechnol. Adv., 28, 325 (2010).

    Article  CAS  PubMed  Google Scholar 

  7. C. Tangsadthakun, S. Kanokpanont, N. Sanchavanakit, T. Banaprasert, and S. Damrongsakkul, J. Metals, Mater. Min., 16, 37 (2017).

    Google Scholar 

  8. S. P. Zhong, Y. Z. Zhang, and C. T. Lim, Wiley Interdiscip. Rev.: Nanomed. Nanobiotechnol., 2, 510 (2010).

    CAS  Google Scholar 

  9. A. Chaudhari, K. Vig, D. Baganizi, R. Sahu, S. Dixit, V. Dennis, S. Singh, and S. Pillai, Int. J. Mol. Sci., 17, 1974 (2016).

    Article  PubMed Central  CAS  Google Scholar 

  10. N. I. A. Mahboob Morshed, S. R. Chowdhury, and B. H. I. Ruszymah, Regen. Res., 3, 17 (2014).

    Google Scholar 

  11. K. Ghosal, M. S. Latha, and S. Thomas, Eur. Polym. J., 60, 58 (2014).

    Article  CAS  Google Scholar 

  12. A. Arabi, E. Boggs, and M. Patel, Surf. Innov., 2, 47 (2014).

    Article  CAS  Google Scholar 

  13. E. Luong-Van, L. Grøndahl, K. N. Chua, K. W. Leong, V. Nurcombe, and S. M. Cool, Biomaterials, 27, 2042 (2006).

    Article  CAS  PubMed  Google Scholar 

  14. M. Chen, M. Chopra, and S. Bhowmick, MRS Online Proceedings Library Archive, 1235 (2009).

  15. A. K. Ekaputra, G. D. Prestwich, S. M. Cool, and D. W. Hutmacher, Biomaterials, 32, 8108 (2011).

    Article  CAS  PubMed  Google Scholar 

  16. E. Vatankhah, D. Semnani, M. P. Prabhakaran, M. Tadayon, S. Razavi, and S. Ramakrishna, Acta Biomater., 10, 709 (2014).

    Article  CAS  PubMed  Google Scholar 

  17. E. K. Brenner, J. D. Schiffman, E. A. Thompson, L. J. Toth, and C. L. Schauer, Carbohydr. Polym., 87, 926 (2012).

    Article  CAS  Google Scholar 

  18. M. N. Collins and C. Birkinshaw, Carbohydr. Polym., 92, 1262 (2013).

    Article  CAS  PubMed  Google Scholar 

  19. K. Y. Lee, L. Jeong, Y. O. Kang, S. J. Lee, and W. H. Park, Adv.Drug Deliv. Rev., 61, 1020 (2009).

    Article  CAS  PubMed  Google Scholar 

  20. M. Arnal-Pastor, C. M. Ramos, M. P. Garnés, M. M. Pradas, and A. V. Lluch, Mater. Sci. Eng.: C, 33, 4086 (2013).

    Article  CAS  Google Scholar 

  21. A. L. McNulty, T. P. Vail, and V. B. Kraus, Biochimica et Biophysica Acta (BBA)-Biomembranes, 1712, 212 (2005).

    Article  CAS  Google Scholar 

  22. K. A. Naidu, Nutr. J., 2, 7 (2003).

    Article  PubMed  PubMed Central  Google Scholar 

  23. D. J. MacKay and A. L. Miller, Altern. Med. Rev., 8, 359 (2003).

    PubMed  Google Scholar 

  24. M. Stevanović, J. Savić, B. Jordović, and D. Uskoković, Colloids Surf. B: Biointerfaces, 59, 215 (2007).

    Article  PubMed  CAS  Google Scholar 

  25. L. Avizheh, T. Peirouvi, K. Diba, and A. Fathi-Azarbayjani, Ther. Deliv., 10, 757 (2019).

    Article  CAS  PubMed  Google Scholar 

  26. R. Najafi-Taher, M. A. Derakhshan, R. Faridi-Majidi, and A. Amani, RSC Adv., 5, 50462 (2015).

    Article  CAS  Google Scholar 

  27. A. Chanda, J. Adhikari, A. Ghosh, S. R. Chowdhury, S. Thomas, P. Datta, and P. Saha, Int. J. Biol. Macromol., 116, 774 (2018).

    Article  CAS  PubMed  Google Scholar 

  28. M. Hu, E. E. Sabelman, Y. Cao, J. Chang, and V. R. Hentz, J. Biomed. Mater. Res. Part B: Appl.Biomater., 67, 586 (2003).

    Article  CAS  Google Scholar 

  29. A. C. Bean, A. J. Almarza, and K. A. Athanasiou, Proc. Inst. Mech. Eng. H: J. Eng. Med., 220, 439 (2006).

    Article  CAS  Google Scholar 

  30. P. L. Lu, J. Y. Lai, D. H. K. Ma, and G. H. Hsiue, J. Biomater. Sci., Polym. Ed., 19, 1 (2008).

    Article  Google Scholar 

  31. J. J. Young, K. M. Cheng, T. L. Tsou, H. W. Liu, and H. J. Wang, J. Biomater. Sci., Polym. Ed., 15, 767 (2004).

    Article  CAS  Google Scholar 

  32. M. N. Collins and C. Birkinshaw, J. Appl. Polym. Sci., 104, 3183 (2007).

    Article  CAS  Google Scholar 

  33. C. Pattamaprom, W. Hongrojjanawiwat, P. Koombhongse, P. Supaphol, T. Jarusuwannapoo, and R. Rangkupan, Macromol. Mater. Eng., 291, 840 (2006).

    Article  CAS  Google Scholar 

  34. C. J. Luo, E. Stride, and M. Edirisinghe, Macromolecules, 45, 4669 (2012).

    Article  CAS  Google Scholar 

  35. Z. Wang, Y. Qian, L. Li, L. Pan, L. W. Njunge, L. Dong, and L. Yang, J. Biomater. Appl., 30, 686 (2016).

    Article  CAS  PubMed  Google Scholar 

  36. N. Mangır, A. J. Bullock, S. Roman, N. Osman, C. Chapple, and S. MacNeil, Acta Biomater., 29, 188 (2016).

    Article  PubMed  PubMed Central  CAS  Google Scholar 

  37. S. N. Park, H. J. Lee, K. H. Lee, and H. Suh, Biomaterials, 24, 1631 (2003).

    Article  CAS  PubMed  Google Scholar 

  38. J. F. Kirk, G. Ritter, I. Finger, D. Sankar, J. D. Reddy, J. D. Talton, C. Nataraj, S. Narisawa, J. L. Millan, and R. R. Cobb, Biomatter, 3, e25633 (2013).

    Article  PubMed  PubMed Central  Google Scholar 

  39. H. M. Liou, L. R. Rau, C. C. Huang, M. R. Lu, and F. Y. Hsu, J. Nanomater., 2013, 6 (2013).

    Article  CAS  Google Scholar 

  40. N. Gokalp, C. Ulker, and Y. A. Guvenilir, J. Polym. Mater., 33, 87 (2016).

    CAS  Google Scholar 

  41. A. Abdolmaleki and Z. Mohamadi, Colloid Polym. Sci., 291, 1999 (2013).

    Article  CAS  Google Scholar 

  42. S. H. Chen, C. H. Chen, K. T. Shalumon, and J. P. Chen, Int. J. Nanomed., 9, 4079 (2014).

    Article  CAS  Google Scholar 

  43. N. Ashwinkumar, S. Maya, and R. Jayakumar, RSC Adv., 4, 49547 (2014).

    Article  CAS  Google Scholar 

  44. H. Liu, K. Li, L. Lan, J. Ma, Y. Zeng, L. Xu, and D. Wu, J. Mater. Chem. B, 2, 5238 (2014).

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  45. A. Umer, S. Naveed, N. Ramzan, M. S. Rafique, and M. Imran, Matéria (Rio de Janeiro), 19, 197 (2014).

    Article  Google Scholar 

  46. C. Y. Panicker, H. T. Varghese, and D. Philip, Spectrochim. Acta Part A: Mol. Biomol. Spectrosc., 65, 802 (2006).

    Article  CAS  Google Scholar 

  47. R. Othayoth, P. Mathi, K. Bheemanapally, L. Kakarla, and M. Botlagunta, J. Microencapsul., 32, 578 (2015).

    Article  CAS  PubMed  Google Scholar 

  48. M. M. Sk and C. Y. Yue, J. Mater. Chem. A, 2, 2830 (2014).

    Article  CAS  Google Scholar 

  49. J. H. Yang, S. Y. Lee, Y. S. Han, K. C. Park, and J. H. Choy, Bull. Korean Chem. Soc., 24, 499 (2003).

    Article  CAS  Google Scholar 

  50. T. Yokoyama, KONA Powder and Particle J., 23, 7 (2005).

    Article  CAS  Google Scholar 

  51. E. Bayrak, B. Ozcan, and C. Erisken, J. Polym. Eng., 37, 99 (2017).

    Article  CAS  Google Scholar 

  52. A. Diaconu, L. E. Nita, M. Bercea, A. P. Chiriac, A. G. Rusu, and D. Rusu, Biochem. Eng. J., 125, 135 (2017).

    Article  CAS  Google Scholar 

  53. M. Van Beek, A. Weeks, L. Jones, and H. Sheardown, J. Biomater. Sci., Polym. Ed., 19, 1425 (2008).

    Article  CAS  Google Scholar 

  54. S. R. Son, N. T. B. Linh, H. M. Yang, and B. T. Lee, Sci. Technol. Adv. Mater., 14, 015009 (2013).

    Article  PubMed  PubMed Central  CAS  Google Scholar 

  55. L. Li, Y. Qian, C. Jiang, Y. Lv, W. Liu, L. Zhong, K. Cai, S. Li, and L. Yang, Biomaterials, 33, 3428 (2012).

    Article  CAS  PubMed  Google Scholar 

  56. C. Gandhimathi, X. H. E. Neo, P. Jayaraman, J. R. Venugopal, S. Ramakrishna, and S. D. Kumar, J. Drug Metab. Toxicol., 5, 177 (2015).

    Google Scholar 

  57. S. Xu, J. Li, A. He, W. Liu, X. Jiang, J. Zheng, C. C. Han, B. S. Hsiao, B. Chu, and D. Fang, Polymer, 50, 3762 (2009).

    Article  CAS  Google Scholar 

  58. T. G. Kim, H. J. Chung, and T. G. Park, Acta Biomater., 4, 1611 (2008).

    Article  CAS  PubMed  Google Scholar 

  59. D. R. Figueira, S. P. Miguel, K. D. de Sá, and I. J. Correia, Int. J. Biol. Macromol., 93, 1100 (2016).

    Article  CAS  PubMed  Google Scholar 

  60. M. Jaganjac, L. Milković, A. Čipak Gašparović, M. Mozetič, N. Recek, N. Žarković, and A. Vesel, Mater. Tehnol., 46, 53 (2012).

    CAS  Google Scholar 

  61. M. Lebourg, J. R. Rochina, T. Sousa, J. Mano, and J. L. G. Ribelles, J. Biomed. Mater. Res. Part A, 101, 518 (2013).

    Article  CAS  Google Scholar 

  62. B. Tavsanli and O. Okay, Eur. Polym. J., 94, 185 (2017).

    Article  CAS  Google Scholar 

  63. B. R. Mintz and J. A. Cooper Jr., J. Biomed. Mater. Res. Part A, 102, 2918 (2014).

    Article  CAS  Google Scholar 

  64. K. Ghosal, S. Thomas, N. Kalarikkal, and A. Gnanamani, J. Polym. Res., 21, 410 (2014).

    Article  CAS  Google Scholar 

  65. X. Zhao, Y. S. Lui, P. W. J. Toh, and S. C. J. Loo, Materials, 7, 7398 (2014).

    Article  PubMed  PubMed Central  Google Scholar 

  66. S. Yan, Q. Zhang, J. Wang, Y. Liu, S. Lu, M. Li, and D. L. Kaplan, Acta Biomater., 9, 6771 (2013).

    Article  CAS  PubMed  Google Scholar 

  67. J. H. Collier, J. P. Camp, T. W. Hudson, and C. E. Schmidt, J. Biomed. Mater. Res., 50, 574 (2000).

    Article  CAS  PubMed  Google Scholar 

  68. K. Ghosal, A. Manakhov, L. Zajíčková, and S. Thomas, Aaps Pharmscitech, 18, 72 (2017).

    Article  CAS  PubMed  Google Scholar 

  69. S. Deepthi, K. Jeevitha, M. N. Sundaram, K. P. Chennazhi, and R. Jayakumar, Chem. Eng. J., 260, 478 (2015).

    Article  CAS  Google Scholar 

  70. K. M. Choi, Y. K. Seo, H. H. Yoon, K. Y. Song, S. Y. Kwon, H. S. Lee, and J. K. Park, J. Biosci. Bioeng., 105, 586 (2008).

    Article  CAS  PubMed  Google Scholar 

  71. J. Y. Zhang, B. A. Doll, E. J. Beckman, and J. O. Hollinger, Tissue Eng., 9, 1143 (2003).

    Article  CAS  PubMed  Google Scholar 

  72. S. Sridhar, J. R. Venugopal, and S. Ramakrishna, J. Biomed. Mater. Res. Part A, 103, 3431 (2015).

    Article  CAS  Google Scholar 

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Authors and Affiliations

  1. Department of Biomedical Engineering, Faculty of New Sciences and Technologies, Semnan University, Semnan, 3513119111, Iran

    Mahsa Janmohammadi

  2. Faculty of Materials and Metallurgical Engineering, Semnan University, Semnan, 3513119111, Iran

    Mohammad Sadegh Nourbakhsh

  3. National Cell Bank, Pasteur Institute, Tehran, 1316943551, Iran

    Shahin Bonakdar

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  1. Mahsa Janmohammadi
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  2. Mohammad Sadegh Nourbakhsh
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  3. Shahin Bonakdar
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Correspondence to Mohammad Sadegh Nourbakhsh.

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Janmohammadi, M., Nourbakhsh, M.S. & Bonakdar, S. Electrospun Skin Tissue Engineering Scaffolds Based on Polycaprolactone/Hyaluronic Acid/L-ascorbic Acid. Fibers Polym 22, 19–29 (2021). https://doi.org/10.1007/s12221-021-0036-8

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