Fibers and Polymers

, Volume 19, Issue 3, pp 477–488 | Cite as

Porous Laponite/Poly(L-lactic acid) Membrane with Controlled Release of TCH and Efficient Antibacterial Performance

  • Qingyan Peng
  • Pengjun Xu
  • Shili Xiao


Fabrication of porous polymer membrane with controlled drug release and efficient antibacterial performances is of great interest in biomedical fields. In this study, Laponite (LAP) nanodisks were first used to encapsulate a model antibiotic drug, tetracycline hydrochloride (TCH). Then, drug-loaded LAP nanodisks with an optimized loading efficiency (85.3 %) were mixed with poly(L-lactic acid) (PLLA) polymer to form drug-loaded composite porous membrane via solvent coasting. The structure, morphology and swelling property of the porous membranes formed with varied solvent ratio of methylene dichloride (DCM) and dimethyl formamide (DMF) in the mixture solvent were characterized using scanning electron microscopy (SEM), Fourier transform infrared spectroscopy and swelling test. In vitro drug release behavior, the cytotoxicity and the antibacterial activity of drug-loaded composite membranes were evaluated. Results showed that the TCH release was dependent on the physical structure of PLLA membrane and the presence of LAP nanodisks effectively weakened the initial burst release of TCH, and improved the sustained release property of porous PLLA membrane. The released TCH of TCH/LAP/PLLA3:1 and TCH/LAP/PLLA4:1 was 10.0 % and 5.3 % within initial 1 h, respectively. More importantly, the porous TCH/LAP/PLLA membrane was cytocompatible and displayed considerable antibacterial activity, solely associated with the loaded TCH drug, confirming its potential utility in wound dressings and tissue engineering.


Poly(L-lactic acid) Tetracycline hydrochloride Laponite Controlled release Antibacterial activity 


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. 1.
    S. U. Nandanwar, K. Coldsnow, V. Utgikar, P. Sabharwall, and D. E. Aston, Chem. Eng. J., 306, 369 (2016).CrossRefGoogle Scholar
  2. 2.
    J. Fan, J. Luo, X. Chen, and Y. Wan, J. Chromatogr. A, 1448, 121 (2016).CrossRefGoogle Scholar
  3. 3.
    Y. He and T. Leïchlé, Sensors Actuat. B-Chem., 239, 628 (2016).CrossRefGoogle Scholar
  4. 4.
    T. Phaechamud and S. Chitrattha, Mat. Sci. Eng. C-Mater., 61, 744 (2016).CrossRefGoogle Scholar
  5. 5.
    A. Wagner, V. Poursorkhabi, A. K. Mohanty, and M. Misra, ACS Sustain. Chem. Eng., 2, 1976 (2014).CrossRefGoogle Scholar
  6. 6.
    M. A. Rahman, J. Wang, C. Zhang, A. Olah, and E. Baer, Eur. Polym. J., 83, 99 (2016).CrossRefGoogle Scholar
  7. 7.
    P. Gibson, H. Schreuder-Gibson, and D. Rivin, Colloid Surf. A-Physicochem. Eng. Asp., 187, 469 (2001).CrossRefGoogle Scholar
  8. 8.
    A. Fiamingo and S. P. Campana-Filho, Carbohydr. Polym., 143, 155 (2016).CrossRefGoogle Scholar
  9. 9.
    H. Li, X. Zhao, P. Wu, S. Zhang, and B. Geng, J. Mater. Sci., 51, 3211 (2016).CrossRefGoogle Scholar
  10. 10.
    W. C. Hung, L. H. Lin, W. C. Tsen, H. S. Shie, H. L. Chiu, T. C. K. Yang, and C. C. Chen, Eur. Polym. J., 67, 166 (2015).CrossRefGoogle Scholar
  11. 11.
    J. Hu, X. Sun, H. Ma, C. Xie, E. Chen, and P. X. Ma, Biomaterials, 31, 7971 (2010).CrossRefGoogle Scholar
  12. 12.
    B. Dhandayuthapani, Y. Yoshida, T. Maekawa, and D. S. Kumar, Int. J. Polym. Sci., 2011, 1 (2011).CrossRefGoogle Scholar
  13. 13.
    J. P. Standord, M. C. Soto, P. H. Pfromm, and M. E. Rezac, Catal. Today, 268, 19 (2016).CrossRefGoogle Scholar
  14. 14.
    L. Zhao, M. Li, M. Liu, Y. Zhang, C. Wu, and Y. Zhang, J. Hazard. Mater., 301, 233 (2016).CrossRefGoogle Scholar
  15. 15.
    Y. Miyoshi, K. Miyajima, H. Saito, H. Kudo, T. Takeuchi, I. Karube, and K. Mitsubayashi, Sensor. Actuat. B-Chem., 142, 28 (2009).CrossRefGoogle Scholar
  16. 16.
    T. Luo, O. David, Y. Gendel, and M. Wessling, J. Power Sources, 312, 45 (2016).CrossRefGoogle Scholar
  17. 17.
    K. M. Nampoothiri, N. R. Nair, and R. P. John, Bioresource Technol., 101, 8493 (2010).CrossRefGoogle Scholar
  18. 18.
    J. Hu, C. Xie, H. Ma, B. Yang, P. X. Ma, and Y. E. Chen, PLoS One, 7, e35580 (2012).CrossRefGoogle Scholar
  19. 19.
    Q. Liu, S. Tian, C. Zhao, X. Chen, L. Lei, Z. Wang, and P. X. Ma, Acta Biomater., 26, 105 (2015).CrossRefGoogle Scholar
  20. 20.
    J. Zhao and R. M. Wilkins, J. Agr. Food Chem., 53, 4076 (2005).CrossRefGoogle Scholar
  21. 21.
    S. Jiang, J. Lv, M. Ding, Y. Li, H. Wang, and S. Jiang, Mater. Sci. Eng. C-Mater., 59, 86 (2016).CrossRefGoogle Scholar
  22. 22.
    A. K. Gaharwar, P. Schexnailder, V. Kaul, O. Akkus, D. Zakharov, S. Seifert, and G. Schmidt, Adv. Funct. Mater., 19, 1(2009).Google Scholar
  23. 23.
    P. J. Schexnailder, A. K. Gaharwar, R. L. Bartlett II, B. L. Seal, and G. Schmidt, Macromol. Biosci., 10, 1416 (2010).CrossRefGoogle Scholar
  24. 24.
    D. W. Thompson and J. T. Butterworth, J. Colloid Interf. Sci., 151, 236 (1992).CrossRefGoogle Scholar
  25. 25.
    H. Jung, H. M. Kim, Y. B. Choy, S. J. Hwang, and J. H. Choy, Int. J. Pharmaceut., 349, 283 (2008).CrossRefGoogle Scholar
  26. 26.
    C. Viseras, P. Cerezo, R. Sanchez, I. Salcedo, and C. Aguzzi, Appl. Clay Sci., 48, 292 (2010).CrossRefGoogle Scholar
  27. 27.
    H. Jung, H. M. Kim, Y. B. Choy, S. J. Hwang, and J. H. Choy, Appl. Clay Sci., 40, 99 (2008).CrossRefGoogle Scholar
  28. 28.
    M. Gon?alves, P. Figueira, D. Maciel, J. Rodrigues, X. Shi, H. Tomás, and Y. Li, Macromol. Biosci., 14, 110 (2013).CrossRefGoogle Scholar
  29. 29.
    S. Wang, Y. Wu, R. Guo, Y. Huang, S. Wen, M. Shen, J. Wang, and X. Shi, Langmuir, 29, 5030 (2013).CrossRefGoogle Scholar
  30. 30.
    M. Gon?alves, P. Figueira, D. Maciel, J. Rodrigues, X. Qu, C. Liu, H. Tomás, and Y. Li, Acta Biomater., 10, 300 (2014).CrossRefGoogle Scholar
  31. 31.
    X. Xiao, R. Castro, D. Maciel, M. Gon?alves, X. Shi, J. Rodrigues, and H. Tomás, Mater. Sci. Eng. C-Mater., 60, 348 (2016).CrossRefGoogle Scholar
  32. 32.
    G. Wang, D. Maciel, Y. Wu, J. Rodrigues, X. Shi, Y. Yuan, C. Liu, H. Tomás, and Y. Li, Acs Appl. Mater. Inter., 6, 16687 (2014).CrossRefGoogle Scholar
  33. 33.
    Y. Wu, R. Guo, S. Wen, M. Shen, M. Zhu, J. Wang, and X. Shi, J. Mater. Chem. B, 2, 7410 (2014).CrossRefGoogle Scholar
  34. 34.
    G. Chen, D. Li, J. Li, X. Cao, J. Wang, X. Shi, and R. Guo, New J. Chem., 39, 2847 (2015).CrossRefGoogle Scholar
  35. 35.
    W. Shao, H. Liu, S. Wang, J. Wu, M. Huang, H. Min, and X. Liu, Carbohydr. Polym., 145, 114 (2016).CrossRefGoogle Scholar
  36. 36.
    F. B. D. Sousa, M. F. Oliveira, I. S. Lula, M. T. C. Sansiviero, M. E. Cortés, and R. D. Sinisterra, Vib. Spectrosc., 46, 57 (2008).CrossRefGoogle Scholar
  37. 37.
    S. Wang, F. Zheng, Y. Huang, Y. Fang, M. Shen, M. Zhu, and X. Shi, Acs Appl. Mater. Inter., 4, 6393 (2012).CrossRefGoogle Scholar
  38. 38.
    D. Garlotta, J. Polym. Environ., 9, 63 (2001).CrossRefGoogle Scholar
  39. 39.
    T. H. Young and L. W. Chen, Desalination, 103, 233 (1995).CrossRefGoogle Scholar
  40. 40.
    Y. H. Yu, C. Y. Lin, J. M. Yeh, and W. H. Lin, Polymer, 44, 3553 (2003).CrossRefGoogle Scholar
  41. 41.
    J. S. Chen, S. L. Tu, and R. Y. Tsay, J. Taiwan Inst. Chem. E., 41, 229 (2010).CrossRefGoogle Scholar
  42. 42.
    Y. Li, D. Maciel, H. Tomás, J. Rodrigues, H. Ma, and X. Shi, Soft Matter, 7, 6231 (2011).CrossRefGoogle Scholar
  43. 43.
    R. Qi, R. Guo, M. Shen, X. Cao, L. Zhang, J. Xu, J. Yu, and X. Shi, J. Mater. Chem., 20, 10622 (2010).CrossRefGoogle Scholar
  44. 44.
    F. D. Lowy, The New Eng. J. Med., 339, 520 (1998).CrossRefGoogle Scholar
  45. 45.
    K. Kim, Y. K. Luu, C. Chang, D. Fang, B. S. Hsiao, B. Chu, and M. Hadjiargyrou, J. Control. Release, 98, 47 (2004).CrossRefGoogle Scholar

Copyright information

© The Korean Fiber Society and Springer Science+Business Media B.V., part of Springer Nature 2018

Authors and Affiliations

  1. 1.School of Textile Science and EngineeringWuhan Textile UniversityWuhanP. R. China
  2. 2.Faculty of Clothing and DesignMinjiang UniversityFuzhouP. R. China
  3. 3.Key Laboratory of Green Processing and Functional Textiles of New Textile Materials, Ministry of EducationWuhan Textile UniversityWuhanP. R. China

Personalised recommendations