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Transcriptomic Analysis Reveals the Effects of Microgravity on Rice Calli on Board the Chinese Spaceship Shenzhou 8

  • Jing Jin
  • Haiying Chen
  • Weiming Cai
Original Article
  • 50 Downloads
Part of the following topical collections:
  1. Approaching the Chinese Space Station - Microgravity Research in China

Abstract

Rice calli were loaded onto the stationary and rotating platforms of a biological incubator (SIMBOX) during spaceflight and ground control experiments. The calli in the SIMBOX were fixed with RNAlater in space after a 324-h spacecraft flight, as well as on the ground at the same time point in a ground control experiment. Microgravity-responsive (MR) transcripts were identified by a comparison of the spaceflight controls (F-μg) with the 1-g ground controls (G-1g) and 1-g inflight controls (F-1g). MapMan analysis was used to classify 955 MR transcripts. These transcripts mainly belonged to the following categories: cell wall modification and metabolism, glycolysis and the tricarboxylic acid (TCA) cycle, transcription factors, protein modification and degradation, hormone metabolism and signalling, calcium regulation, receptor-like kinase activity, and transporters. Here, we focused on the effects of microgravity on the plant cell wall and discussed the relationship between the variation in the cell wall and plant cell growth under microgravity.

Keywords

Microgravity Spaceflight Transcriptome Oryza sativa Callus cells Cell wall Aquaporin 

Abbreviations

MR

Microgravity responsive

SIMBOX

Science in Microgravity Box

2,4-D

2,4-dichlorophenoxyacetic acid

F-μg

Spaceflight controls

F-1g

1-g inflight controls

G-1g

1-g ground controls

Notes

Acknowledgements

The Technology and Engineering Center for Space Utilization (Chinese Academy of Sciences) is acknowledged for project coordination. The spaceflight equipment was developed by the Shanghai Institute of Technical Physics (Chinese Academy of Sciences) Microarray analysis was conducted by Shanghai Biochip Co., Ltd., and Xue Li (Institute of Plant Physiology and Ecology, SIBS, CAS) helped with the data analysis.

Author Contributions

Conception, design, experimentation, dataanalysis and manuscript writing: Jing Jin Experimentation: Haiying Chen. Conception, design and final approval of manuscript: Weiming Cai.

Funding Information

This work was supported by the National Natural Science Foundation of China (Grant Nos. U1738107, 31570859, 31500236 and 31600684), Strategic Priority Research Program of the Chinese Academy of Sciences (Grant Nos. XDA04020202-15, XDA04020415), and the China Manned Space Flight Technology Project.

Compliance with Ethical Standards

Conflict of interests

All authors reviewed the manuscript and declared no competing financial interests.

Supplementary material

12217_2018_9633_MOESM1_ESM.docx (1.4 mb)
(DOC 1.40 MB)

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Copyright information

© Springer Science+Business Media B.V., part of Springer Nature 2018
corrected publication 2018

Authors and Affiliations

  1. 1.Laboratory of Photosynthesis and Environment, CAS Center for Excellence in Molecular Plant Sciences, Shanghai Institute of Plant Physiology and EcologyChinese Academy of SciencesShanghaiChina

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