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Characterization and expression profiles of small heat shock proteins in the marine red alga Pyropia yezoensis

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Abstract

Small heat shock proteins (sHSPs) are found in all three domains of life (Bacteria, Archaea, and Eukarya) and play a critical role in protecting organisms from a range of environmental stresses. However, little is known about their physiological functions in red algae. Therefore, we characterized the sHSPs (PysHSPs) in the red macroalga Pyropia yezoensis, which inhabits the upper intertidal zone where it experiences fluctuating stressful environmental conditions on a daily and seasonal basis, and examined their expression profiles at different developmental stages and under varying environmental conditions. We identified five PysHSPs (PysHSP18.8, 19.1, 19.2, 19.5, and 25.8). Real-time quantitative reverse transcription polymerase chain reaction (qRT-PCR) analysis showed that expression of the genes PysHSP18.8, PysHSP19.5, and PysHSP25.8 was repressed at all the developmental stages under normal conditions, whereas PysHSP19.1 and PysHSP19.2 were overexpressed in mature gametophytes and sporophytes. Exposure of the gametophytes to high temperature, oxidative stress, or copper significantly increased the mRNA transcript levels of all the five genes, while exogenous application of the ethylene precursor 1-aminocylopropane-1-carboxylic acid (ACC) significantly increased the expression levels of PysHSP19.2, PysHSP19.5, and PysHSP25.8. These findings will help to further our understanding of the role of PysHSP genes and provide clues about how Pyropia species can adapt to the stressful conditions encountered in the upper intertidal zone during their life cycle.

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Acknowledgments

We are grateful to Drs. Katsutoshi Arai and Takafumi Fujimoto (Hokkaido University, Japan) for kindly providing LightCycler 480 system. This study was supported by a grant-in-aid for Young Scientists (B) (16K18740 to T.U.) from the Japan Society for the Promotion of Science (JSPS).

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Authors and Affiliations

  1. Division of Marine Life Science, Faculty of Fisheries Sciences, Hokkaido University, Hakodate, 041-8611, Japan

    Toshiki Uji, Yohei Gondaira & Hiroyuki Mizuta

  2. Section of Food Sciences, Institute for Regional Innovation, Hirosaki University, Aomori, Aomori, 038-0012, Japan

    Satoru Fukuda & Naotsune Saga

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  1. Toshiki Uji
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  2. Yohei Gondaira
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  3. Satoru Fukuda
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  4. Hiroyuki Mizuta
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Correspondence to Toshiki Uji.

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Uji, T., Gondaira, Y., Fukuda, S. et al. Characterization and expression profiles of small heat shock proteins in the marine red alga Pyropia yezoensis. Cell Stress and Chaperones 24, 223–233 (2019). https://doi.org/10.1007/s12192-018-00959-9

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