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HSP60 expression profile under different extreme temperature stress in albino northern snakehead, Channa argus

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Cell Stress and Chaperones Aims and scope

Abstract

The great albino northern snakehead, Channa argus, is one of the most important economical fish in China. In the present study, cDNA encoding heat shock protein 60 (HSP60) was cloned and characterized. The cDNA was 2462 bp, containing an open reading frame (ORF) encoding a 575-amino-acids polypeptide of 61.10 kDa (theoretical isoelectric point [pI]: 5.66). BLAST analysis showed that AcaHSP60 was highly similar with other HSP60s, and three conserved amino acid blocks and characteristic motifs or domains defined as HSP60 protein family signatures. Genomic DNA analysis showed that AcaHSP60 had ten exons in the coding region (from 94 to 336 bp). Changes in AcaHSP60 gene expression profiles in albino C. argus experimentally exposed to different temperature stress (8.5, 26, and 37 °C) was investigated. Quantitative real-time PCR and western blot analysis revealed that tissue-specific AcaHSP60 expressions were in the spleen, muscle, liver, kidney, heart and brain. Expression was highly significantly stimulated after heat shock (37 °C), but showed no significant differences after cold treatment (8.5 °C) except in the brain. In summary, these results showed that AcaHSP60 was significantly tissue specific and indicate that AcaHSP60 expression might be sensitive to thermal resistance in albino C. argus.

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References

  • Adoutte A, Balavoine G, Lartillot N, Lespinet O, Prud’homme B, de Rosa R (2000) The new animal phylogeny: reliability and implications. Proc. Natl. Acad. Sci. USA 97(9):4453–4456. https://doi.org/10.1073/pnas.97.9.4453

    Article  PubMed  CAS  Google Scholar 

  • Cheng QT, Zheng BS (1987) Systematic Search of Fish in China. Science Press, Beijing, p p456

    Google Scholar 

  • Cheng MY, Hartl FU, Horwich AL (1990) The mitochondrial chaperon in HSP60 is required for its own assembly. Nature 348(6300):455–458. https://doi.org/10.1038/348455a0

    Article  PubMed  CAS  Google Scholar 

  • Courtenay WRJ, Williams JD (2004) Snakeheads (Pisces, Channidae): A biological synopsis and risk assessment, US Department of the Interior, US Geological Survey, Circular p43

  • Eggert-Kruse W, Batschulat K, Demirakca T, Strowitzki T (2015) Male immunity to the chlamydial 60 kDa heat shock protein (HSP60) associated with semen quality? Andrologia 47(1):66–76. https://doi.org/10.1111/and.12224

    Article  PubMed  CAS  Google Scholar 

  • Habich C, Burkart V (2007) Heat shock protein 60: regulatory role on innate immune cells. Cell Mol Life Sci 647:42–51

    Google Scholar 

  • Intergovernmental Panel on Climate Change (IPCC) (2007) Fourth assessment report climate change 2007: synthesis report. IPCC, Geneva. https://doi.org/10.1017/CBO9780511546013

    Book  Google Scholar 

  • Itoh H, Komatsuda A, Ohtani H, Wakui H, Imai H, Sawada KI, Otaka M, Ogura M, Suzuki A, Hamada F (2002) Mammalian HSP60 is quickly sorted into the mitochondria under conditions of dehydration. Eur J Biochem 269(23):5931–5938. https://doi.org/10.1046/j.1432-1033.2002.03317.x

    Article  PubMed  CAS  Google Scholar 

  • Jia WZ, Shang N, Guo QL (2010) Molecular cloning of rhamnose-binding lectin gene and its promoter region from snakehead Channa argus. Fish Physiol Biochem 36(3):451–459. https://doi.org/10.1007/s10695-009-9315-9

    Article  PubMed  CAS  Google Scholar 

  • Johnson RB, Fearon K, Mason T, Jindal S (2003) Cloning and characterization of the yeast chaperonin HSP60 gene. Gene 84:295–300

    Article  Google Scholar 

  • Liu FR (1999) The farming technology of Channa argus. Fishery Guide Rich 4:20

    Google Scholar 

  • Liu J, Cui Y, Liu J (2000) Resting metabolism and heat increment of feeding in mandarin fish (Siniperca chuatsi) and Chinese snakehead (C. argus). Comp Biochem Physiol 127(2):131–138. https://doi.org/10.1016/S1095-6433(00)00246-4

    Article  CAS  Google Scholar 

  • Livak KJ, Schmittgen TD (2001) Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) method. Methods 25(4):402–408. https://doi.org/10.1006/meth.2001.1262

    Article  PubMed  CAS  Google Scholar 

  • Mohanty S, Mohanty BP (2009) GlobalClimate change: a cause of concern. Natl Acad Sci Lett 32(5–6):149–156

  • Nakano K, Iwama GK (2002) The 70-kDa heat shock protein response in two intertidal sculpins, Oligocottus maculosus and O. snyderi: relationship of hsp70 and thermal tolerance. Comp Biochem Physiol A Mol Integr Physiol 133(1):79–94. https://doi.org/10.1016/S1095-6433(02)00115-0

    Article  PubMed  Google Scholar 

  • Quan G, Duan J, Ladd T, Krell PJ (2017) Identification and expression analysis of multiple small heat shock protein genes in spruce budworm, Choristoneura fumiferana (L.) Cell Stress Chaperones:1–14

  • Ricci C, Barone G, Bulone D, Burgio G, Carrotta R, Librizzi F, Gammazza AM, Mangione MR, Piccionello AP, Biagio PLS, Spinello A, Spinozzi F, Vilasi S, Ortore MG (2016) Structure and stability of Hsp60 and Groel in solution. Biophys J 110(3):368a

  • Santoro MG (2000) Heat shock factors and the control of the stress response. Biochem Pharmacol 59(1):55–63. https://doi.org/10.1016/S0006-2952(99)00299-3

    Article  PubMed  CAS  Google Scholar 

  • Shi HN, Liu Z, Zhang JP, Kang YJ, Wang JF, Huang JQ, Wang WM (2015) Effect of heat stress on heat-shock protein (Hsp60) mRNA expression in rainbow trout Oncorhynchus mykiss. Genet Mol Biol 14:5280–5286

    CAS  Google Scholar 

  • Shi J, Fu M, Zhao C, Zhou F, Yang Q, Qiu L (2016) Characterization and function analysis of Hsp60 and Hsp10 under different acute stresses in black tiger shrimp, Penaeus monodon. Cell Stress Chaperones 21(2):295–312. https://doi.org/10.1007/s12192-015-0660-6

    Article  PubMed  CAS  Google Scholar 

  • SQ S, Xiong B (2011) Artificial reproduction test of Opniocepnalus argus var Kimnra. Reservoir Fisheries 1:19–20

    Google Scholar 

  • Wang PF, Xu P, Zhou L, Zeng S, Li G (2017) Molecular cloning, characterization, and expression analysis of HSP60 in mandarin fish Siniperca chuatsi. Isr J Aquacult-Bamidgeh 69:1–13

    Google Scholar 

  • Xie Y, Song L, Weng Z, Liu S, Liu Z (2015) Hsp90, Hsp60 and sHsp families of heat shock protein genes in channel catfish and their expression after bacterial infections. Fish Shellfish Immunol 44(2):642–651

    Article  PubMed  CAS  Google Scholar 

  • Xu D, Sun L, Liu S, Zhang L, Ru X, Zhao Y, Yang H (2014a) Molecular cloning of heat shock protein 10 (Hsp10) and 60 (Hsp60) cDNAs and their expression analysis under thermal stress in the sea cucumber Apostichopus japonicus. Comp Biochem Physiol B: Biochem Mol Biol 171:49–57. https://doi.org/10.1016/j.cbpb.2014.03.009

    Article  CAS  Google Scholar 

  • Xu Y, Zheng G, Dong S, Liu G, Yu X (2014b) Molecular cloning, characterization and expression analysis of HSP60, HSP70 and HSP90 in the golden apple snail, Pomacea canaliculata. Fish Shellfish Immunol 41(2):643–653. https://doi.org/10.1016/j.fsi.2014.10.013

    Article  PubMed  CAS  Google Scholar 

  • Zhou AG, Wang C, Jiang WZ, Li ZG, Chen YF, Xie SL, Luo JZ, Zou JX (2017a) Genetic comparison of two color morphs of northern snakehead (Channa argus) and genetic relatednessamong the family Chnannidae. Mitochondrial DNA Part A 28(6):971–973. https://doi.org/10.1080/24701394.2016.1186668

    Article  CAS  Google Scholar 

  • Zhou AG, Xie SL, Wang ZL, Junaid M, Fan LF, Wang C, Ye Q, Chen YF, Pei DS, Zou JX (2017b) Molecular cloning, characterization and expression analysis of heat shock protein 90 in albino northern snakehead Channa argus. Gene 626:173–181. https://doi.org/10.1016/j.gene.2017.04.039

    Article  PubMed  CAS  Google Scholar 

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Acknowledgements

We express our appreciation to our anonymous reviewers for providing valuable comments on the manuscript.

Funding

This work was supported by the Science and Technology Planning Project of Guangdong Province (2017A020225035; 2016A020210141); Produce-learn-research Project of Guangdong Province (2011B090400270); Fund Fostering Talents for Young Scholars of South China Agricultural University (201707 N025); Talent introduction special funds of South China Agricultural University and Scientific Research Staring Foundation for Young Scholars of College of Marine Sciences.

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Correspondence to Jixing Zou.

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All of the procedures and animal handling were performed in accordance with the guide for the Chinese Association for Laboratory Animal Sciences. Approval of the study was obtained from the Animal Ethics Committee of South China Agricultural University.

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The authors declare that they have no conflicts of interest.

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Zhou, A., Xie, S., Wang, Z. et al. HSP60 expression profile under different extreme temperature stress in albino northern snakehead, Channa argus. Cell Stress and Chaperones 23, 791–796 (2018). https://doi.org/10.1007/s12192-017-0869-7

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