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Frequency of KRAS mutations in adult Korean patients with acute myeloid leukemia

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Abstract

Mutation of KRAS genes occurs with a frequency of 0.5–32 % in AML. In the present study, mutations of KRAS codon 12, 13, and 61 were detected by pyrosequencing and direct sequencing in AML. Seven KRAS mutations (7/123, 5.7 %) were detected. The most common mutation was a G-to-A transition in the second base of KRAS codon 13. No mutations were detected in KRAS codon 61. Combinations of KRAS and FLT3 mutation were not found in the same patient. There was no statistically significant difference between patients with KRAS mutations and patients with wild-type KRAS in terms of sex, age, CBC at diagnosis, CD34 positivity, MPO positivity, FLT3 mutation, karyotype, progression-free survival, and overall survival, although this may be attributable to the small sample size. To our knowledge, this is the first report of the detection of KRAS mutation in Asian AML patients using pyrosequencing and direct sequencing. These two methods showed identical efficiencies in their ability to detect KRAS mutations in 84 patients.

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Acknowledgments

Our trial was financially supported by QIAGEN Korea and Samil Pharmacy & Trading Company.

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Authors and Affiliations

  1. Department of Laboratory Medicine, Gachon University Gil Medical Center, 1198 Guwol-dong, Namdong-gu, Incheon, 405-760, Korea

    Mi-Jung Park, Soon-Ho Park, Pil-Whan Park, Yiel-Hea Seo, Kyung-Hee Kim, Ji-Hun Jeong, Moon Jin Kim & Jeong-Yeal Ahn

  2. Departments of Internal Medicine, Gachon University Gil Medical Center, Incheon, Korea

    Jae Hoon Lee, Jinny Park & Junshik Hong

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  1. Mi-Jung Park
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Correspondence to Jeong-Yeal Ahn.

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Park, MJ., Park, SH., Park, PW. et al. Frequency of KRAS mutations in adult Korean patients with acute myeloid leukemia. Int J Hematol 98, 549–557 (2013). https://doi.org/10.1007/s12185-013-1446-1

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  • DOI: https://doi.org/10.1007/s12185-013-1446-1

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