In this study, the concentrations of macro- and microelement in different tissues (liver, gill, and muscle) of fish collected (Mugil cephalus) in Pontal Bay (Ilhéus, Bahia) were determined. A simplex-centroid mixture design was used to obtain the mixture applied to the decomposition of the samples. The elementary determination was made by microwave-induced plasma optical emission spectrometry (MIP OES), and the Kohonen self-organizing map (KSOM) was applied as an exploratory analysis tool. The accuracy of the procedure was assessed using certified reference material. Recoveries were obtained in the range of 99–103% and without a statistical difference (95% confidence level). Precision was estimated from the relative standard deviation, which ranged from 0.3 to 2.2%, and the limits of detection (mg kg−1) were 0.145 (Cu), 1.53 (Fe), 1.61 (K), 0.250 (Mg), 0.040 (Mn), 4.84 (P), and 2.12 (Zn). The KSOM provided efficient and precise separation of the studied tissues into three groups. The concentrations of Cu, Fe, and Zn were higher in the liver, while in the gills, they were the Mn.
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The authors received funding from Fundação de Amparo à Pesquisa do Estado da Bahia (FAPESB), Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) (Grant numbers 306698/2019-6 and 421694/2018-1), and Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) – Finance Code 001 under the W.N.G. grant fellowship.
Conflict of Interest
Clissiane Soares Viana Pacheco declares that she has no conflict of interest. Floriatan dos Santos Costa declares that he has no conflict of interest. Wesley Nascimento Guedes declares that he has no conflict of interest. Marina Santos de Jesus declares that she has no conflict of interest. Thiago Pereira das Chagas declares that he has no conflict of interest. Ana Maria Pinto dos Santos declares that she has no conflict of interest. Daniel de Castro Lima declares that he has no conflict of interest. Erik Galvão Paranhos da Silva declares that he has no conflict of interest.
All applicable international, national, and/or institutional guidelines for the care and use of animals were followed. This study was approved by the Research Ethics Committee of the State University of Santa Cruz (No 07/2016) and National System for the Management of Genetic Heritage and Associated Traditional Knowledge (No A2C2576).
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Pacheco, C.S.V., Costa, F.S., Guedes, W.N. et al. Application of Mixture Design and Kohonen Neural Network for Determination of Macro- and Microelement in Mullet (Mugil cephalus) by MIP OES. Food Anal. Methods (2021). https://doi.org/10.1007/s12161-021-01969-7
- Mugil cephalus
- Mixture design
- Kohonen self-organizing map
- MIP OES