Development and Validation of an Analytical Method for the Quantification of Arabinose, Galactose, Glucose, Sucrose, Fructose, and Maltose in Fruits, Vegetables, and Their Products


The paper presents a method for the simultaneous determination of arabinose, galactose, glucose, sucrose, fructose, and maltose in fruits, vegetables, and their products using ion chromatography-pulsed amperometric detection on a gold working electrode. Sample preparation involved simple extraction, dilution with water, and filtration before injection into the system. Separation of the analytes was performed with gradient elution on a Thermo Scientific CarboPac PA20 Analytical Column (3.0 × 150 mm) and a Guard column (2 mm × 50 mm) with a mobile phase. The optimized parameters were an eluent concentration of 10 mmol/L, a column temperature of 30 °C, and a flow rate of 0.4 mL/min. In methodological evaluation, parameters (selectivity, linearity, limit of detection (LOD), limit of quantification (LOQ), relative standard deviation (RSD), recovery percentages, and CV%) resulted in compliance with reference values at each level of fortification. All validation parameters were acceptable in the characterization of sugars in fruits, vegetables, and their products. After method validation, we analyzed the sugar content in fresh and dried jujube. The method was accurate, reliable, and reproducible and can be used for the determination of arabinose, galactose, glucose, sucrose, fructose and maltose in fruits, vegetables, and their products.

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  1. Antczak-Chrobot A, Bąk P, Wojtczak M (2018) The use of ionic chromatography in determining the contamination of sugar by-products by nitrite and nitrate. Food Chem 240:648–654

    CAS  Article  Google Scholar 

  2. Cataldi TRI, Margiotta G, Iasi L, Chio BD, Xiloyannis C and Bufo S A J A C (2000). "Determination of sugar compounds in olive plant extracts by anion-exchange chromatography with pulsed amperometric detection." 72(16): 3902–3907

  3. Cinquina AL, Cali A, Longo F, Santis LD, Severoni A and Abballe F (2004). "Determination of biogenic amines in fish tissues by ion-exchange chromatography with conductivity detection." 1032(1–2): 73–77

  4. Costacurta M, Direnzo L, Sicuro L, Gratteri S, De Lorenzo A and Docimo R (2014). "Dental caries and childhood obesity: analysis of food intakes, lifestyle." 15(4): 343-348

  5. Lamb JD, Myers GS and Edge N (1993). "Ion chromatographic analysis of glucose, fructose, and sucrose concentrations in raw and processed vegetables." (9): 9

  6. Debebe A, Temesgen S, Redi-Abshiro M, Chandravanshi BS, Ele E (2018) Improvement in analytical methods for determination of sugars in fermented alcoholic beverages. Journal of Analytical Methods in Chemistry 2018:1–10

    Article  Google Scholar 

  7. Genualdi S, Jeong N, DeJager L (2018) Determination of endogenous concentrations of nitrites and nitrates in different types of cheese in the United States: method development and validation using ion chromatography. Food Additives & Contaminants: Part A 35(4):614–622

    CAS  Google Scholar 

  8. Fialho TL, Nogueira MH, Moreau A, Delaplace G, Júnior P P D S P (2019). "Sugar type matters in spray drying: Homogeneous distribution in milk powder favors repulsive interactions between proteins." 22: 100132

  9. Gomis DB, Alvarez MDG, Alonso JJM and Vallina ANJC (1988). "Determination of sugars and alcohols in apple juice and cider by high performance liquid chromatography." 25(8): 701-706

  10. Hou LJ, He SL, Zhao H and Jie W (2017). "Sugar and non-volatile organic acids analysis of jujube in different areas."

  11. Kashimura J, Nagai Y and Ebashi T (2004). "Reducer of blood glucose level increase reducer of body fat accumulation and food material."

  12. Loncaric A, Dugalic K, Mihaljevic I, Jakobek L and Pilizota V (2014). "effects of sugar addition on total polyphenol content and antioxidant activity of frozen and freeze-dried apple purée." 62(7): 1674-1682

  13. Ma CM, Zhu SH, Zhang LL, Sun ZZ and Chen CB (2014). "Simultaneous separation and determination of fructose, sorbitol, glucose and sucrose in fruits by HPLC-ELSD." 145(feb.15): 784-788

  14. Malongo TK, Patris S, Macours P, Cotton F, Nsangu J and Kauffmann JM (2008). "Highly sensitive determination of iodide by ion chromatography with amperometric detection at a silver-based carbon paste electrode." 76(3): 0-547

  15. Martens DA and Frankenberger WT (1991). "On-line solid-phase extraction of soil auxins produced from exogenously-applied tryptophan with ion-suppression reverse-phase HPLC analysis." 32(9-10): 417-422

  16. Monti L, Negri S, Meucci A, Stroppa A, Galli A and Contarini G (2017). "Lactose, galactose and glucose determination in naturally "lactose free" hard cheese: HPAEC-PAD method validation." 220(apr.1): 18-24

  17. Ni C, Zhu B, Wang N, Wang M, Chen S, Zhang J and Zhu Y (2016). "Simple column-switching ion chromatography method for determining eight monosaccharides and oligosaccharides in honeydew and nectar." 194(MAR.1): 555-560

  18. Prichard R, Rossi M, Muir J, Yao C, Whelan K, Lomer M (2016) Fermentable oligosaccharide, disaccharide, monosaccharide and polyol content of foods commonly consumed by ethnic minority groups in the United Kingdom. Int J Food Sci Nutr 67(4):383–390

    CAS  Article  Google Scholar 

  19. Rostami H and Gharibzahedi SMT (2016). "Microwave-assisted extraction of jujube polysaccharide: optimization, purification and functional characterization." 143: 100-107

  20. Seneviratne J, Holmstrom SD and Cox JA (2000). "Donnan dialysis preconcentration coupled with ion chromatography and electrocatalytic oxidation for the determination of cyanide." 52(6): 1025-1031

  21. Silva DGD, Carvalho ALD, Araújo EJ, Rocha ME, Santos DLS (2020) Development of an analytical method for determination of nitrate in leafy vegetables using ion chromatography. Open Access Library Journal 07(6):1–8

    Google Scholar 

  22. Standards I (1994). "Accuracy (trueness and precision) of measurement methods and results - part 6: use in practice of accuracy values."

  23. Vos MB, Kimmons JE, Gillespie C, Welsh J and Blanck HM (2008). "Dietary fructose consumption among US children and adults: the Third National Health and Nutrition Examination Survey CME." 10(7): 160

  24. Yao RQA, Liu HYB, Niu PFA and Qiu NXA (2007). "Ultrasonic-assisted extraction of jujube polysaccharide and analysis in HPLC after derivatization." 4(1):11-17

  25. Zeppa G, Conterno L and Gerbi V (2001). "Determination of organic acids, sugars, diacetyl, and acetoin in cheese by high-performance liquid chromatography." 49(6): 2722-2726

  26. Zhang XY, Tong QY and Ren F (2012). "Manuscript title: influence of glucose, sucrose and trehalose on the freeze-thaw stability of tapioca starch gels." 4(4): 225-230

  27. Zhao D, Feng F, Yuan F, Su J, Cheng Y, Wu H, Song K, Nie B, Yu L and Zhang F (2017). "Simultaneous determination of 13 carbohydrates using high-performance anion-exchange chromatography coupled with pulsed amperometric detection and mass spectrometry." 40(8): 1843-1854

  28. Zhou S, Tang QJ, Luo X, Xue JJ, Liu YF, Yang Y, Zhang JS and Feng M (2012). "Determination of carbohydrates by high performance anion chromatography-pulsed amperometric detection in mushrooms." 14(4): 411-417

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This work was supported by National Key R&D Program of China (2017YFC1601701).

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Correspondence to Xinlian Zhang or Zhenzhen Xu.

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Bingfeng Wang declares that he/she has no conflict of interest. Xue Wang declares that he/she has no conflict of interest. Jun Bei declares that he/she has no conflict of interest. Lei Xu declares that he/she has no conflict of interest. Xinlian Zhang declares that he/she has no conflict of interest. Zhenzhen Xu declares that he/she has no conflict of interest.

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Wang, B., Wang, X., Bei, J. et al. Development and Validation of an Analytical Method for the Quantification of Arabinose, Galactose, Glucose, Sucrose, Fructose, and Maltose in Fruits, Vegetables, and Their Products. Food Anal. Methods (2021).

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  • Ion chromatography
  • Arabinose
  • Galactose
  • Glucose
  • Sucrose
  • Fructose