Selective Solid-Phase Extraction of Sulfonamides from Edible Swine Tissues Using High-Performance Imprinted Polymers

Abstract

In the present study, sulfamethazine-imprinted polymers have been prepared by bulk polymerization method with a living radical photopolymerization using sulfamethazine (SM2) as the template molecule, methacrylic acid (MAA) as the functional monomer, ethylene glycol dimethacrylate (EGDMA) as the cross-linker, and azobisisobutyronitrile (AIBN) as the initiator in the porogenic solvent of acetonitrile. After estimating the recognition properties, the imprinting factor of the polymer reached up to 9.3779. Then, the polymer particles were incorporated into a solid-phase extraction (SPE) column for the selective separation and purification of the sulfonamides (SAs), including sulfadiazine (SD), sulfamerazine (SM1), and SM2, from edible swine tissues. By using the detection method of high-performance liquid chromatography with the optimum SPE conditions, the limits of detection and limits of quantitation were calculated as 0.7–2.3 μg kg−1 and 3.4–4.6 μg kg−1 in the complex samples, respectively. The recovery of SAs ranged from 85.5 to 88.3%. Compared with the commonly used Waters Oasis HLB® column, the proposed selective SM2-MI-SPE column has opened a promising new approach for trace enrichment of the residues. It will facilitate sulfonamide residue analysis in animal tissues.

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Acknowledgments

Special thanks to Dr. Shoulei Yan for his enthusiastic support in the preparation and characterization of the MIPs.

Funding

This study was financially supported by the National Natural Science Foundation of China (31772074) and the Fundamental Research Funds for the Central Universities (2662017PY049, 2017BC010).

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Correspondence to Dapeng Peng.

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Zhaozhou Li declare that he has no conflict of interest. Yulian Wang declare that she has no conflict of interest. Zhenli Liu declare that he has no conflict of interest. Changqing Xie declare that he has no conflict of interest. Dapeng Peng declare that he has no conflict of interest. Zonghui Yuan declare that he has no conflict of interest. There is no professional or other personal interest of any nature or kind in any product, service, and/or company that could be construed as influencing the position presented in, or the review of, the manuscript entitled “Selective solid-phase extraction of sulphonamides from edible swine tissues using high-performance imprinted polymers.”

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This article does not contain any studies with human subjects. All animal experiments that described in the present study were performed in adherence to Huazhong Agricultural University animal experiment center guidelines and approved by Animal Ethics Committee.

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Li, Z., Wang, Y., Liu, Z. et al. Selective Solid-Phase Extraction of Sulfonamides from Edible Swine Tissues Using High-Performance Imprinted Polymers. Food Anal. Methods 13, 1304–1313 (2020). https://doi.org/10.1007/s12161-020-01751-1

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Keywords

  • Molecularly imprinted polymers
  • Sulfonamide·solid-phase extraction
  • Edible swine tissues