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Dual-Mode Aptasensor for SERS and Chiral Detection of Campylobacter jejuni

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Abstract

Campylobacter jejuni (C. jejuni), a foodborne pathogen, is a major contributor to human bacterial gastroenteritis worldwide and detrimental to public health. In this paper, we developed a dual-mode aptasensor for Campylobacter jejuni (C. jejuni) determination. Based on surface-enhanced Raman scattering (SERS) and chiral optical response, the concentrations of C. jejuni in food products can be simultaneously quantified by SERS and chirality methods. Under the optimized conditions, in the ranges of 102–5 × 106 cfu/mL and 102–106 cfu/mL, the concentration of C. jejuni exhibited a good linear relationship (R2 = 0.9905 and 0.9819) with changed SERS signal intensity and chiral signal intensity, respectively. In addition, the applicability of the novel aptasensor was validated by detecting different concentrations of C. jejuni spiked in milk samples. With its satisfactory performances, this bimodal aptasensor is conceivably to be a promising candidate for determining C. jejuni in food products.

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Funding

This research was financially supported by the Natural Science Foundation of Shandong Province (ZR2019BC088 and ZR2018BC064), Funds for Innovation Team of Jinan (2018GXRC004), and Special Funds for Taishan Scholars Project.

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Author notes

  1. Deyun He and Zhengzong Wu contributed equally to this work.

Authors and Affiliations

  1. State Key Laboratory of Biobased Material and Green Papermaking, College of Food Science and Engineering, Qilu University of Technology, Shandong Academy of Sciences, Jinan, 250353, China

    Deyun He, Zhengzong Wu & Bo Cui

  2. State Key Laboratory of Plant Physiology and Biochemistry, College of Biological Sciences, China Agricultural University, Beijing, 100094, China

    Deyun He

  3. College of Biosystems Engineering and Food Science, Zhejiang University, Hangzhou, 310058, China

    Enbo Xu

  4. National Engineering Laboratory of Intelligent Food Technology and Equipment, Zhejiang Key Laboratory for Agro-Food Processing, Fuli Institute of Food Science, Zhejiang University, Hangzhou, 310058, China

    Enbo Xu

Authors
  1. Deyun He
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  2. Zhengzong Wu
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  3. Bo Cui
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  4. Enbo Xu
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Correspondence to Zhengzong Wu or Bo Cui.

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Deyun He declares that she has no conflict of interest. Zhengzong Wu declares that he has no conflict of interest. Bo Cui declares that he has no conflict of interest. Enbo Xu declares that he has no conflict of interest.

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He, D., Wu, Z., Cui, B. et al. Dual-Mode Aptasensor for SERS and Chiral Detection of Campylobacter jejuni. Food Anal. Methods 12, 2185–2193 (2019). https://doi.org/10.1007/s12161-019-01574-9

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