Fast Analysis of Total Polyphenol Content and Antioxidant Activity in Wines and Oenological Tannins Using a Flow Injection System with Tandem Diode Array and Electrochemical Detections
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An analytical method for simultaneous determination of total polyphenol content (TPC) and antioxidant activity (AA) of wines (white and red wines) and oenological tannins, using a flow injection system with sequential diode array and electrochemical amperometry detectors (DAD-ECD), was proposed. The signal at 280 nm provided aggregate data for TPC. The anodic peak related to wine phenolic oxidation was scanned using pulsed integrated amperometry over the potential of 800 mV vs. Ag/AgCl, to obtain AA. Serial dilutions avoided the poisoning at the glassy carbon (GC) electrode and the linear response obtained with both detectors was compared with spectrophotometric assays commonly used in oenology laboratory. Intraday and interday analytical repetitions showed a good repeatability and reproducibility (relative standard deviation RSD < 6% for both detectors), and the satisfactory relationship between the proposed coupled flow injection/DAD-ECD and the classic UV methods (R2TPC = 0.9967; R2DPPH = 0.9621) confirmed the efficacy of flow injection analysis with a coupled detection system, for the reliable quality control of wine and wine-related products.
KeywordsTotal polyphenol content Antioxidant activity Electrochemistry Flow injection DPPH• Method comparison
Authors gratefully acknowledge the HTS Enologia and Laffort companies for having supplied food-grade tannin samples.
Authors A.R. and N.T. had PhD grants by the University of Bologna (Italy) and Penta EU program, respectively. Author V.I.P. was funded by the ERASMUS Mundus Action 2 Project while carrying out this experiment.
Compliance with Ethical Standards
Conflict of Interest
Arianna Ricci declares that she has no conflict of interest. Nemanja Teslic declares that he has no conflict of interest. Violeta-Ivanova Petropolus declares that she has no conflict of interest. Giuseppina Paola Parpinello declares that she has no conflict of interest. Andrea Versari declares that she has no conflict of interest.
This article does not contain any studies with human participants or animals performed by any of the authors.
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