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Food Analytical Methods

, Volume 12, Issue 2, pp 347–354 | Cite as

Fast Analysis of Total Polyphenol Content and Antioxidant Activity in Wines and Oenological Tannins Using a Flow Injection System with Tandem Diode Array and Electrochemical Detections

  • Arianna Ricci
  • Nemanja Teslic
  • Violeta-Ivanova Petropolus
  • Giuseppina Paola ParpinelloEmail author
  • Andrea Versari
Article
  • 211 Downloads

Abstract

An analytical method for simultaneous determination of total polyphenol content (TPC) and antioxidant activity (AA) of wines (white and red wines) and oenological tannins, using a flow injection system with sequential diode array and electrochemical amperometry detectors (DAD-ECD), was proposed. The signal at 280 nm provided aggregate data for TPC. The anodic peak related to wine phenolic oxidation was scanned using pulsed integrated amperometry over the potential of 800 mV vs. Ag/AgCl, to obtain AA. Serial dilutions avoided the poisoning at the glassy carbon (GC) electrode and the linear response obtained with both detectors was compared with spectrophotometric assays commonly used in oenology laboratory. Intraday and interday analytical repetitions showed a good repeatability and reproducibility (relative standard deviation RSD < 6% for both detectors), and the satisfactory relationship between the proposed coupled flow injection/DAD-ECD and the classic UV methods (R2TPC = 0.9967; R2DPPH = 0.9621) confirmed the efficacy of flow injection analysis with a coupled detection system, for the reliable quality control of wine and wine-related products.

Keywords

Total polyphenol content Antioxidant activity Electrochemistry Flow injection DPPH• Method comparison 

Notes

Acknowledgments

Authors gratefully acknowledge the HTS Enologia and Laffort companies for having supplied food-grade tannin samples.

Funding

Authors A.R. and N.T. had PhD grants by the University of Bologna (Italy) and Penta EU program, respectively. Author V.I.P. was funded by the ERASMUS Mundus Action 2 Project while carrying out this experiment.

Compliance with Ethical Standards

Conflict of Interest

Arianna Ricci declares that she has no conflict of interest. Nemanja Teslic declares that he has no conflict of interest. Violeta-Ivanova Petropolus declares that she has no conflict of interest. Giuseppina Paola Parpinello declares that she has no conflict of interest. Andrea Versari declares that she has no conflict of interest.

Ethical Approval

This article does not contain any studies with human participants or animals performed by any of the authors.

Informed Consent

Not applicable.

Supplementary material

12161_2018_1366_MOESM1_ESM.jpg (231 kb)
ESM 1 Schematic representation of the flow injection system with tandem diode-array and electrochemical detections device used for analysis. (JPG 231 kb)
12161_2018_1366_MOESM2_ESM.jpg (114 kb)
ESM 2 Bland – Altman Plot describing correlation between UV/ 280 nm and flow injection /ECD data used for polyphenolic quantifications. (JPG 113 kb)

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2018

Authors and Affiliations

  • Arianna Ricci
    • 1
  • Nemanja Teslic
    • 2
  • Violeta-Ivanova Petropolus
    • 3
  • Giuseppina Paola Parpinello
    • 1
    Email author
  • Andrea Versari
    • 1
  1. 1.Department of Agricultural and Food SciencesUniversity of BolognaCesenaItaly
  2. 2.Institute of Food TechnologyUniversity of Novi SadNovi SadSerbia
  3. 3.Faculty of AgricultureUniversity “Goce Delčev”ŠtipRepublic of Macedonia

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