Determination of Five Plant Growth Regulator Containing Carboxyl in Bean Sprouts Based on Chemical Derivatization by GC-MS
An efficient and sensitive analytical method for the simultaneous determination of five plant growth regulators (PGRs) has been successfully developed, including 3-Indoleacetic acid (IAA), 2-Naphthylacetic acid (2-NAA), Beta-indolebutyric acid (IBA), 2-(2,4-dichlorophenoxy), acetic acid (2.4-D), and 4-Chlorophenoxyacetic acid (4-CPA) in bean sprouts. Using 10% boron trifluoride of methanol as the derived reagent and the reaction was derivatized at 70 °C for 30 min, the derivatives of five PGRs have been completely separated within 19 min. It has a linear relationship in the concentration range of 0.01–0.20 mg/kg and the correlation coefficients (r2) in excess of 0.997. The average recoveries were ranged from 72.7–98.6% at three spiking different concentration levels of 0.05, 0.10, and 0.15 mg/kg, respectively. The relative standard deviations (RSDs) were ranged from 1.2 to 9.0%. The limits of detection and the limits of quantification were ranged from 0.00024 to 0.00172 mg/kg and 0.0008–0.0052 mg/kg, respectively. On the basis of these, the measurement has been successfully applied to the analysis of the exogenous phytohormones in the bean sprouts. Except for 4-CPA and IAA, the rest of the analytes were undetected in samples. In sum, the current method can be used for accurate determination and confirmation of PGRs in many other crops, such as bean sprouts.
KeywordsBean sprouts Plant growth regulator Derivatization Gas chromatography-mass spectrometry
This work was supported by the Social Development Foundation of Shaanxi Province, China (2017SF-384 and 2017SF-383).
Compliance with Ethical Standards
Conflict of Interest
Guofu Qin declares that he has no conflict of interest. Keting Zou declares that she has no conflict of interest. Li Tian declares that she has no conflict of interest. Yongbo Li declares that she has no conflict of interest.
This article does not contain any studies with human participants or animals performed by any of the authors.
- Agricultural and Veterinary Chemicals Code Instrument No. 4 (MRL Standard) (2012) Available from http://www.apvma.gov.au/residues/ (accessed Jan 2016
- Banerjee K, Oulkar DP, Patil SB, Jadhav MR, Dasgupta S, Patil SH, Bal S, Adsule PG (2009) Multiresidue determination and uncertainty analysis of 87 pesticides in mango by liquid chromatography-tandem mass spectrometry. Chin J Health Lab Technol 57:4068–4078Google Scholar
- Cho SK, El-Aty AMA, Park KH, Park JH, Assayed ME, Jeong YM, Park YS, Shim JH (2013) Simple multiresidue extraction method for the determination of fungicides and plant growth regulator in bean sprouts using low temperature partitioning and tandem mass spectrometry. Food Chem 136:1414–1420CrossRefGoogle Scholar
- EPA (2012) Protection of environment. Available from http://www.epa.gov/pesticides/food/viewtols.htm (accessed Jan 2016)
- Health and Safety Executive (2014) Available from https://secure.pesticides.gov.uk/MRLs/ (accessed Jan 2016)
- Lin T, Li QW, Liu HC, Fan JL, Yang DS, Li YG (2016) Rapid extraction and determination of exogenous plant growth regulators in fruits and vegetables. Environ Chem 35:57–66Google Scholar
- Liu YM, Liu HL, Ji WL, Ruan LP, Zhou CM (2015) Simultaneous determination of 7 plant growth regulators residues in bean sprout by QuEChERS - HPLC-MS/MS. Chin J Health Lab Technol 25:1880–1883Google Scholar
- Mou YL, Guo DH, Ding ZP (2013) Determination of 7 plant growth regulator residues in Orange by solid phase extraction and liquid chromatography-tandem mass spectrometry. J Instrum Anal 32:935–940Google Scholar
- Rademacher W, Brahm L (2002) Plant growth regulators, 1st edn. Wiley-VCH Verlag GmbH and Co. KgaA, WeinheimGoogle Scholar
- Regulations on the Control of Agricultural Chemicals (1977) Available from http://www.asianlii.org/cn/legis/cen/laws/rotcoac491/ (accessed Jan 2016)
- SAC (2014) Maximum residue limits for pesticides in food (GB 2763–2014). The Ministry of Health and agricuture of China, BeijingGoogle Scholar
- Shi X, Jin F, Huang Y, Du X, Li C, Wang M, Shao H, Jin M, Wang J (2012) Simultaneous determination of five plant growth regulators in fruits by modified quick, easy, cheap, effective, rugged, and safe (QuEChERS) extraction and liquid chromatography−tandem mass spectrometry. J Agric Food Chem 60:60–65CrossRefGoogle Scholar
- Swiader JM, Ware GW (2002) Producing vegetable crops, 5th edn. Interstate Publisher, Inc., DanvilleGoogle Scholar
- Zhang Y, Li Y, Hu Y, Li G, Chen Y (2010) Preparation of magnetic indole-3-acetic acid imprinted polymer beads with 4-vinylpyridine and b-cyclodextrin asbinary monomer via microwave heating initiated polymerization and their application to trace analysis of auxins in plant tissues. J Chromatogr A 1217:7337–7344CrossRefGoogle Scholar