Food Analytical Methods

, Volume 11, Issue 9, pp 2590–2596 | Cite as

Analytical Approach for Detection of Ergosterol in Mushrooms Based on Modification Free Electrochemical Sensor in Organic Solvents

  • Vesna Vukojević
  • Sladjana Djurdjić
  • Ľubomír Švorc
  • Tanja Ćirković Veličković
  • Jelena Mutić
  • Dalibor M. Stanković


Ergosterol (Erg) is the major sterol component in the plasma membranes of fungi. The main goal of this work was to develop rapid, reliable, and cost-effective method toward Erg detection based on application of unmodified boron-doped diamond electrode. Several organic solvents were tested (acetonitrile, dimethyformamide, dimethylsulfoxide, dichloromethane), and it was found that best analytical characteristics were achieved using acetonitrile containing 0.1 M tetrabutylammonium hexa-fluorophosphate as supporting electrolyte. Oval shaped and well-defined oxidation peak was recorded at potential of around 1.17 V versus Ag/Ag+ non-aqueous reference electrode. Under optimized experimental conditions, linear working range from 2 to 200 μM was estimated, with limit of detection of 0.7 μM, using square wave voltammetry. Negligible effect of possible interfering compound was observed. Proposed methodology was successfully applied for estimation of Erg content in mushrooms extract with satisfactory results in comparison with spectrophotometric procedure. Obtained results clearly show that developed analytical methodology can be adequate replacement for the, up to date, used methods and open-novel approach for ergosterol detection.


Ergosterol Boron-doped diamond electrode Mushrooms 


Funding Information

This work was supported by Magbiovin project (FP7-ERAChairs-Pilot Call-2013, Grant agreement: 621375), by the Ministry of Education, Science and Technology, the Republic of Serbia (Project No. OI 172030), and the Grant Agency of the Slovak Republic (Grant No. 1/0489/16).

Compliance with Ethical Standards

Conflict of Interest

Vesna Vukojević declares that she has no conflict of interest. Sladjana Djurdjić declares that she has no conflict of interest. Ľubomír Švorc declares that he has no conflict of interest. Tanja Ćirković-Veličković declares that she has no conflict of interest. Jelena Mutić declares that she has no conflict of interest. Dalibor M. Stanković declares that he has no conflict of interest.

Ethical Approval

This article does not contain any studies with human or animal subjects performed by any of the authors.

Informed Consent

Informed consent is not applicable for the nature of this study.


  1. Brycht M, Kaczmarska K, Uslu B, Ozkan SA, Skrzypek S (2016) Sensitive determination of anticancer drug imatinib in spiked human urine samples by differential pulse voltammetry on anodically pretreated boron-doped diamond electrode. Diam Relat Mater 68:13–22CrossRefGoogle Scholar
  2. Chen C, Tripp CP (2012) A comparison of the behavior of cholesterol, 7-dehydrocholesterol and ergosterol in phospholipid membranes. Biochim Biophys Acta 1818:1673–1681CrossRefGoogle Scholar
  3. Deroco PCB, Vicentini FC, Oliveira GG, Rocha-Filho RC, Fatibello-Filho O (2014) Square-wave voltammetric determination of hydroxychloroquine in pharmaceutical and synthetic urine samples using a cathodically pretreated boron-doped diamond electrode. J Electroanal Chem 719:19–23CrossRefGoogle Scholar
  4. Hashim SNNS, Schwarz LJ, Danylec B, Mitri K, Yang Y, Boysen RI, Hearn MTW (2016) Recovery of ergosterol from the medicinal mushroom, Ganoderma tsugae var. Janniae, with a molecularly imprinted polymer derived from a cleavable monomer-template composite. J Chromatogr A 1468:1–9CrossRefGoogle Scholar
  5. Heleno SA, Prieto MA, Barros L, Rodrigues AR, Barreiro MF, Ferreira ICFR (2016) Optimization of microwave-assisted extraction of ergosterol from Agaricus bisporus L. by-products using response surface methodology. Food Bioprod Process 100:25–35CrossRefGoogle Scholar
  6. Kul D, Gumustas M, Uslu B, Ozkan SA (2010) Electroanalytical characteristics of antipsychotic drug ziprasidone and its determination in pharmaceuticals and serum samples on solid electrodes. Talanta 82:286–295CrossRefGoogle Scholar
  7. Kuzmanovi D, Stankovi DM, Manojlovi D, Kalcher K, Rogli G (2015) Baicalein: main active flavonoid from Scutellaria baicalensis voltammetric sensing in human samples using boron doped diamond electrode. Diam Relat Mater 58:35–39CrossRefGoogle Scholar
  8. Li SP, Li P, Lai CM, Gong YX, Kan KKW, Dong TTX, Tsim KWK, Wang YT (2004) Simultaneous determination of ergosterol, nucleosides and their bases from natural and cultured cordyceps by pressurised liquid extraction and high-performance liquid chromatography. J Chromatogr A 1036:239–243CrossRefGoogle Scholar
  9. Lourenção BC, Medeiros RA, Rocha-Filho RC, Mazo LH, Fatibello-Filho O (2009) Simultaneous voltammetric determination of paracetamol and caffeine in pharmaceutical formulations using a boron-doped diamond electrode. Talanta 78:748–752CrossRefGoogle Scholar
  10. Lü Y, Zhu Y, Cheng G, Dong S (1999) Study of electrochemical behavior of ergosterol by in situ thin layer circular dichroic spectroelectrochemistry. Electroanalysis 11:601–605CrossRefGoogle Scholar
  11. Mannock DA, Lewis RNAH, McElhaney RN (2010) A calorimetric and spectroscopic comparison of the effects of ergosterol and cholesterol on the thermotropic phase behavior and organization of dipalmitoylphosphatidylcholine bilayer membranes. Biochim Biophys Acta 1798:376–388CrossRefGoogle Scholar
  12. Muniroh MS, Sariah M, Zainal Abidin MA, Lima N, Paterson RRM (2014) Rapid detection of ganoderma-infected oil palms by microwave ergosterol extraction with HPLC and TLC. J Microbiol Methods 100:143–147CrossRefGoogle Scholar
  13. Parsi Z, Górecki T (2006) Determination of ergosterol as an indicator of fungal biomass in various samples using non-discriminating flash pyrolysis. J Chromatogr A 1130:145–150CrossRefGoogle Scholar
  14. Pastinen O, Nyyssölä A, Pihlajaniemi V, Sipponen MH (2017) Fractionation process for the protective isolation of ergosterol and trehalose from microbial biomass. Process Biochem 58:217–223CrossRefGoogle Scholar
  15. Porep JU, Erdmann ME, Körzendörfer A, Kammerer DR, Carle R (2014) Rapid determination of ergosterol in grape mashes for grape rot indication and further quality assessment by means of an industrial near infrared/visible (NIR/VIS) spectrometer—a feasibility study. Food Control 43:142–149CrossRefGoogle Scholar
  16. Sabatini K, Mattila J-P, Kinnunen PKJ (2008) Interfacial behavior of cholesterol, ergosterol, and lanosterol in mixtures with DPPC and DMPC. Biophys J 95:2340–2355CrossRefGoogle Scholar
  17. Sartori ER, Medeiros RA, Rocha-Filho RC, Fatibello-Filho O (2010) Square-wave voltammetric determination of propranolol and atenolol in pharmaceuticals using a boron-doped diamond electrode. Talanta 81:1418–1424CrossRefGoogle Scholar
  18. Stankovi DM, Kalcher K (2016) Amperometric quantification of the pesticide ziram at boron doped diamond electrodes using flow injection analysis. Sensors Actuators B Chem 233:144–147CrossRefGoogle Scholar
  19. Stanković DM (2015) Sensitive voltammetric determination of thymol in essential oil of Carum copticum seeds using boron-doped diamond electrode. Anal Biochem 486:1–4CrossRefGoogle Scholar
  20. Stanković DM (2017) Electroanalytical approach for quantification of pesticide Maneb. Electroanalysis 29:352–357CrossRefGoogle Scholar
  21. Stanković DM, Kalcher K (2015) The immunosuppressive drug—Rapamycin—electroanalytical sensing using boron-doped diamond electrode. Electrochim Acta 168:76–81CrossRefGoogle Scholar
  22. Uslu B, Topal BD, Ozkan SA (2008) Electroanalytical investigation and determination of pefloxacin in pharmaceuticals and serum at boron-doped diamond and glassy carbon electrodes. Talanta 74:1191–1200CrossRefGoogle Scholar
  23. Yuan J-P, Wang J-H, Liu X, Kuang H-C, Zhao S-Y (2007) Simultaneous determination of free ergosterol and ergosteryl esters in Cordyceps sinensis by HPLC. Food Chem 105:1755–1759CrossRefGoogle Scholar
  24. Zhang Z, Tan W, Hu Y, Li G (2011) Simultaneous determination of trace sterols in complicated biological samples by gas chromatography–mass spectrometry coupled with extraction using β-sitosterol magnetic molecularly imprinted polymer beads. J Chromatogr A 1218:4275–4283CrossRefGoogle Scholar
  25. Zhou J, Guan W, Liang C, Yang F, Ma Y (2015) Determination of ergosterol in alfalfa by gas chromatography–mass spectrometry. Anal Lett 48:241–247CrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2018

Authors and Affiliations

  • Vesna Vukojević
    • 1
  • Sladjana Djurdjić
    • 1
  • Ľubomír Švorc
    • 2
  • Tanja Ćirković Veličković
    • 3
    • 4
  • Jelena Mutić
    • 3
    • 4
  • Dalibor M. Stanković
    • 1
    • 5
  1. 1.Innovation Center of the Faculty of ChemistryUniversity of BelgradeBelgradeSerbia
  2. 2.Institute of Analytical Chemistry, Faculty of Chemical and Food TechnologySlovak University of Technology in BratislavaBratislavaSlovak Republic
  3. 3.Faculty of ChemistryUniversity of BelgradeBelgradeSerbia
  4. 4.Ghent University Global CampusIncheonSouth Korea
  5. 5.The “Vinča” Institute of Nuclear SciencesUniversity of BelgradeBelgradeSerbia

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