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One-Step Core/Multishell Quantum Dots-Based Fluoroimmunoassay for Screening of Deoxynivalenol in Maize

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Abstract

Deoxynivalenol (DON) is a protein synthesis inhibitor produced by the Fusarium species, which frequently contaminates grains used for human or animal consumption, thereby necessitating their effective monitoring and control. Antibody-based immunoassays provide efficient and cheap methods for screening this mycotoxin. To achieve high sensitivity and avoid enzymatic reaction, a new highly sensitive monoclonal antibody (MAb) and a fluorescent probe consisting of amino-functionalized core/multishell quantum dots (QDs), CdSe/CdS/Cd0.5Zn0.5S/ZnS, bio conjugated to 3-HS-DON-BSA were prepared; then, a sensitive and reliable direct competitive fluorescent-labeled immunosorbent assay (dcFLISA) was developed. Under optimized conditions, the 50% inhibition concentration (IC50) value of the dcFLISA was 5.6 μg/kg with limit of detection of 12.2 μg/kg in maize samples. The recoveries ranged from 77.3 to 84.3% with coefficients of variation (CV%) values below 10%. Good correlation between the dcFLISA and HPLC-MS/MS results in the naturally contaminated maize demonstrated that the dcFLISA was a rapid and sensitive with high-performance and reliable method for the detection of DON. The potential of this method to function as a simple and efficient strategy for small molecule on-site screening might be applied in the future.

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References

  • Algar WR, Susumu K, Delehanty JB, Medintz IL (2011) Semiconductor quantum dots in bioanalysis: crossing the valley of death. Anal Chem 83(23):8826–8837

    Article  CAS  PubMed  Google Scholar 

  • An L, Wang Y, Pan Y, Tao Y, Chen D, Liu Z, Yang W, Peng D, Yuan Z (2016) Development and validation of a sensitive indirect competitive enzyme-linked immunosorbent assay for the screening of florfenicol and thiamphenicol in edible animal tissue and feed. Food Anal Methods 9(9):2434–2443

    Article  Google Scholar 

  • Beloglazova NV, Speranskaya ES, De SS, Hens Z, Abe S, Goryacheva IY (2012) Quantum dot based rapid tests for zearalenone detection. Anal Bioanal Chem 403(10):3013–3024

    Article  CAS  PubMed  Google Scholar 

  • Binder EM, Tan LM, Chin LJ, Handl J, Richard J (2007) Worldwide occurrence of mycotoxins in commodities, feeds and feed ingredients. Anim Feed Sci Tech 137(3):265–282

    Article  CAS  Google Scholar 

  • Casale WL, Pestka JJ, Hart LP (1988) Enzyme-linked immunosorbent-assay employing monoclonal-antibody specific for deoxynivalenol (vomitoxin) and several analogs. J Agric Food Chem 36(3):663–668

    Article  CAS  Google Scholar 

  • Dong G, Pan Y, Wang Y, Ahmed S, Liu Z, Peng D, Yuan Z (2017) Preparation of a broad-spectrum anti-zearalenone and its primary analogues antibody and its application in an indirect competitive enzyme-linked immunosorbent assay. Food Chem 247:8–15

    Article  CAS  PubMed  Google Scholar 

  • Duan H, Chen XL, Xu W, Fu JH, Xiong YH, Wang A (2015) Quantum-dot submicrobead-based immunochromatographic assay for quantitative and sensitive detection of zearalenone. Talanta 132:126–131

    Article  CAS  PubMed  Google Scholar 

  • Garcia-Fernandez J, Trapiella-Alfonso L, Costa-Fernandez JM, Pereiro R, Sanz-Medel A (2014) A quantum dot-based immunoassay for screening of tetracyclines in bovine muscle. J Agric Food Chem 62(7):1733–1740

    Article  CAS  PubMed  Google Scholar 

  • Goodrow MH, Hammock BD (1998) Hapten design for compound-selective antibodies: Elisas for environmentally deleterious small molecules. Anal Chim Acta 376(1):83–91

    Article  CAS  Google Scholar 

  • He Y, Lu HT, Sai LM, Su YY, Hu M, Fan CH, Huang W, Wang LH (2008) Microwave synthesis of water-dispersed CdTe/CdS/ZnS core-shell-shell quantum dots with excellent photostability and biocompatibility. Adv Mater 20(18):3416–3421

    Article  CAS  Google Scholar 

  • Hickert S, Gerding J, Ncube E, Hubner F, Flett B, Cramer B, Humpf HU (2015) A new approach using micro HPLC-MS/MS for multi-mycotoxin analysis in maize samples. Mycotoxin Res 31(2):109–115

    Article  CAS  PubMed  Google Scholar 

  • Jiang WX, Luo PJ, Wang X, Chen X, Zhao YF, Shi W, Wu XP, Wu YN, Shen JZ (2012) Development of an enzyme-linked immunosorbent assay for the detection of nitrofurantoin metabolite, 1-amino-hydantoin, in animal tissues. Food Control 23(1):20–25

    Article  CAS  Google Scholar 

  • Kim KY, Shim WB, Kim JS, Chung DH (2014) Development of a simultaneous lateral flow strip test for the rapid and simple detection of deoxynivalenol and zearalenone. J Food Sci 79(10):2048–2055

    Article  CAS  Google Scholar 

  • Kong D, Liu L, Song S, Zheng Q, Wu X, Kuang H (2017a) Rapid detection of tenuazonic acid in cereal and fruit juice using a lateral-flow immunochromatographic assay strip. Food Agr Immunol 2:1–11

    Google Scholar 

  • Kong D, Xie Z, Liu L, Song S, Kuang H (2017b) Development of ic-ELISA and lateral-flow immunochromatographic assay strip for the detection of citrinin in cereals. Food Agr Immunol 28(3):414–426

    Article  CAS  Google Scholar 

  • Kong D, Xie Z, Liu L, Song S, Kuang H, Cui G, Xu C (2017c) Development of indirect competitive ELISA and lateral-flow immunochromatographic assay strip for the detection of sterigmatocystin in cereal products. Food Agr Immunol 28(2):260–273

    Article  CAS  Google Scholar 

  • Kong D, Xie Z, Liu L, Song S, Zheng Q, Kuang H (2017d) Development of an immunochromatographic assay for the detection of alternariol in cereal and fruit juice samples. Food Agr Immunol 2:1–12

    Google Scholar 

  • Kroutgreenberg ND, Puschner B, Davidson MG, Depeters EJ (2013) Preliminary study to assess mycotoxin concentrations in whole corn in the California feed supply. J Dairy Sci 96(4):2705–2712

    Article  CAS  Google Scholar 

  • Lee HM, Song SO, Cha SH, Wee SB, Bischoff K, Park SW, Son SW, Kang HG, Cho MH (2013) Development of a monoclonal antibody against deoxynivalenol for magnetic nanoparticle-based extraction and an enzyme-linked immunosorbent assay. J Vet Sci 14(2):143–150

    Article  PubMed  PubMed Central  Google Scholar 

  • Li CL, Wen K, Mi TJ, Zhang XY, Zhang HY, Zhang SX, Shen JZ, Wang ZH (2016) A universal multi-wavelength fluorescence polarization immunoassay for multiplexed detection of mycotoxins in maize. Biosens Bioelectron 79:258–265

    Article  CAS  PubMed  Google Scholar 

  • Liu Y, Lu Y, Wang L, Chang F, Yang L (2016) Occurrence of deoxynivalenol in wheat, Hebei Province, China. Food Chem 197 (Pt B) 197:1271–1274

    Article  CAS  Google Scholar 

  • Li Y, Liu G, Fu X, He J, Wang Z, Hou J, Cao X, Shi W, Zhang S (2015) High-sensitive chemiluminescent ELISA method investigation for the determination of deoxynivalenol in rice. Food Analy Methods 8(3):656–660

    Article  Google Scholar 

  • Li YS, Shi WM, Shen JZ, Zhang SX, Cheng LL, Wang ZH (2012) Development of a rapid competitive indirect ELISA procedure for the determination of deoxynivalenol in cereals. Food Agr Immunol 23(1):41–49

    Article  CAS  Google Scholar 

  • Ma M, Wen K, Beier RC, Eremin SA, Li CL, Zhang SX, Shen J, Wang ZH (2016) Chemiluminescence resonance energy transfer competitive immunoassay employing hapten-functionalized quantum dots for the detection of sulfamethazine. ACS Appl Mater Interfaces 8(28):17745–17750

    Article  CAS  PubMed  Google Scholar 

  • Maragos CM, McCormick SP (2000) Monoclonal antibodies for the mycotoxins deoxynivalenol and 3-acetyl-deoxynivalenol. Food Agric Immunol 12(3):181–192

    Article  CAS  Google Scholar 

  • Maragos C, Busman M, Sugita-Konishi Y (2006) Production and characterization of a monoclonal antibody that cross-reacts with the mycotoxins nivalenol and 4-deoxynivalenol. Food Addit Contam 23(8):816–825

    Article  CAS  PubMed  Google Scholar 

  • Mills EC, Alcock SM, Lee HA, Morgan MR (1990) An enzyme-linked immunosorbent assay for deoxynivalenol in wheat, utilizing novel hapten derivatization procedures. Food Agric Immunol 2(3):109–118

    Article  CAS  Google Scholar 

  • Monbaliu S, Van Poucke C, Detavernier C, Dumoulin F, Velde MVD, Schoeters E, Dyck SV, Averkieva O, Peteghem CV, De SS (2010) Occurrence of mycotoxins in feed as analyzed by a multi-mycotoxin LC-MS/MS method. J Agric Food Chem 58(1):66–71

    Article  CAS  PubMed  Google Scholar 

  • Peng D, Chang F, Wang Y, Chen D, Liu Z, Zhou X, Feng L, Yuan Z (2016a) Development of a sensitive monoclonal-based enzyme-linked immunosorbent assay for monitoring T-2 toxin in food and feed. Food Addit Contam A 33(4):683–692

    CAS  Google Scholar 

  • Peng D, Yang B, Pan Y, Wang Y, Chen D, Liu Z, Yang W, Tao Y, Yuan Z (2016b) Development and validation of a sensitive monoclonal antibody-based indirect competitive enzyme-linked immunosorbent assay for the determination of the aflatoxin M1 levels in milk. Toxicon 113:18–24

    Article  CAS  PubMed  Google Scholar 

  • Sanders M, Guo Y, Iyer A, Garcia YR, Galvita A, Heyerick A, Deforce D, Risseeuw MDP, Calenbergh SV, Bracke M, Eremin S, Madder A, De SS (2014) An immunogen synthesis strategy for the development of specific anti-deoxynivalenol monoclonal antibodies. Food Addit Contam A 31(10):1751–1759

    Article  CAS  Google Scholar 

  • Sinha RC, Savard ME, Lau R (1995) Production of monoclonal-antibodies for deoxynivalenol and 15-acetyldeoxynivalenol by ELISA. J Agric Food Chem 43(6):1740–1744

    Article  CAS  Google Scholar 

  • Song S, Liu N, Zhao Z, Ediage EN, Wu S, Sun C, De SS, Wu A (2014) Multiplex lateral flow immunoassay for mycotoxin determination. Anal Chem 86(10):4995–5001

    Article  CAS  PubMed  Google Scholar 

  • Speranskaya ES, Beloglazova NV, Lenain P, De SS, Wang ZH, Zhang SX, Hens Z, Knopp D, Niessner R, Potapkin DV, Goryacheva IY (2014) Polymer-coated fluorescent CdSe-based quantum dots for application in immunoassay. Biosens Bioelectron 53(4):225–231

    Article  CAS  PubMed  Google Scholar 

  • Streit E, Naehrer K, Rodrigues I, Schatzmayr G (2013) Mycotoxin occurrence in feed and feed raw materials worldwide: long-term analysis with special focus on Europe and Asia. J Sci Food Agr 93(12):2892–2899

    Article  CAS  Google Scholar 

  • Sun MM, Du LY, Gao SQ, Bao YH, Wang SH (2010) Determination of 17 beta-oestradiol by fluorescence immunoassay with streptavidin-conjugated quantum dots as label. Steroids 75(6):400–403

    Article  CAS  PubMed  Google Scholar 

  • Wang J, Wang Y, Pan Y, Feng L, Chen D, Liu Z, Peng D, Yuan Z (2016) Preparation of a broadly specific monoclonal antibody-based indirect competitive ELISA for the detection of benzodiazepines in edible animal tissues and feed. Food Anal Methods 9(12):1–13

    Google Scholar 

  • Wei FD, Lin Y, Wu YZ, Sun X, Liu LP, Zhou P, Hu Q (2014) Double shell CdTe/CdS/ZnS quantum dots as a fluorescence probe for quetiapine determination in fumarate quetiapine tablets. Anal Methods 6(2):482–489

    Article  CAS  Google Scholar 

  • Yang D, Geng ZM, Yao JB, Zhang X, Zhang PP, Ma HX (2013) Simultaneous determination of deoxynivalenol, and 15-and 3-acetyldeoxynivalenol in cereals by HPLC-UV detection. World Mycotoxin J 6(2):117–125

    Article  CAS  Google Scholar 

  • Yang QH, Gong XQ, Song T, Yang JM, Zhu SJ, Li YH, Cui Y, Li YX, Zhang BB, Chang J (2011) Quantum dot-based immunochromatography test strip for rapid, quantitative and sensitive detection of alpha fetoprotein. Biosens Bioelectron 30(1):145–150

    Article  CAS  PubMed  Google Scholar 

  • Yu Q, Li H, Li C, Zhang S, Shen J, Wang Z (2015) Gold nanoparticles-based lateral flow immunoassay with silver staining for simultaneous detection of fumonisin B1 and deoxynivalenol. Food Control 54:347–352

    Article  CAS  Google Scholar 

  • Zhang X, Wen K, Wang Z, Jiang H, Beier RC, Shen J (2016) An ultra-sensitive monoclonal antibody-based fluorescent microsphere immunochromatographic test strip assay for detecting aflatoxin M1 in milk. Food Control 60(2):588–595

    Article  CAS  Google Scholar 

  • Zhang X, Yu X, Wen K, Li C, Mujtaba MG, Jiang H, Shi W, Shen J, Wang Z (2017) Multiplex lateral flow immunoassays based on amorphous carbon nanoparticles for detecting three fusantium mycotoxins in maize. J Agric Food Chem 65(36):8063–8071

    Article  CAS  PubMed  Google Scholar 

  • Zhang ZW, Li YY, Li PW, Zhang Q, Zhang W, Hu XF, Ding XX (2014) Monoclonal antibody-quantum dots CdTe conjugate-based fluoroimmunoassay for the determination of aflatoxin B1 in peanuts. Food Chem 146(1):314–319

    Article  CAS  PubMed  Google Scholar 

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Acknowledgements

This work was supported by grants from Natural Science Foundation of China (U1301214 and 31502114) and Special Fund for Agro-scientific Research in the Public Interest (201203040). And we are very thankful to Prof. Sarah De Saeger (Laboratory of Food Analysis, Faculty of Pharmaceutical Sciences, Ghent University) for providing naturally contaminated maize samples.

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Authors and Affiliations

  1. Henan Province Engineering Research Center for Food Safety Control of Processing and Circulation, College of Food Science and Technology, Henan Agricultural University, 63 Nongye Road, Zhengzhou, Henan, 450002, China

    Xiya Zhang

  2. Beijing Advanced Innovation Center for Food Nutrition and Human Health, College of Veterinary Medicine, China Agricultural University, Beijing, 100193, China

    Xiya Zhang, Xuezhi Yu, Jianyi Wang & Zhanhui Wang

  3. Beijing Center for Physical and Chemical Analysis, Hatchery Building B, No. 7 middle Feng Xian Road, Haidian District, Beijing, 100094, China

    Qi Wang

  4. School of the Environment and Safety Engineering, Jiangsu university, 301 Xuefu Road, Zhenjiang, Jiangsu Province, 212013, China

    Hui Meng

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  1. Xiya Zhang
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  4. Qi Wang
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  6. Zhanhui Wang
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Contributions

Xiya Zhang and Xuezhi Yu contributed equally to this paper.

Corresponding author

Correspondence to Zhanhui Wang.

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Conflict of Interest

Xiya Zhang declares that he has no conflict of interest. Xuezhi Yu declares that she has no conflict of interest. Jianyi Wang declares that he has no conflict of interest. Qi wang declares that she has no conflict of interest. Hui Meng declares that he has no conflict of interest. Zhanhui Wang declares that he has no conflict of interest.

Ethical Approval

This article does not contain any studies with human subjects. All animal experiments described in this study were approved by the Animal Care Center of the China Agricultural University, Beijing, China, following all institutional and national guidelines for the care and use of laboratory animals.

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Zhang, X., Yu, X., Wang, J. et al. One-Step Core/Multishell Quantum Dots-Based Fluoroimmunoassay for Screening of Deoxynivalenol in Maize. Food Anal. Methods 11, 2569–2578 (2018). https://doi.org/10.1007/s12161-018-1198-x

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  • DOI: https://doi.org/10.1007/s12161-018-1198-x

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