Multicommutated Flow System for the Determination of Glyphosate Based on Its Quenching Effect on CdTe-Quantum Dots Fluorescence
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Glyphosate is the most widely used herbicide at the moment. It presents a broad spectrum of action, hence its use for many different crops. Regulatory agencies have constantly mentioned the low hazard potential to mammals. However, the International Agency for Research on Cancer concluded in 2015 that glyphosate is “probably carcinogenic to humans.” For this reason, it is important to develop reliable analytical methods to study the fate and levels of glyphosate in environmental samples. In this work, we propose a multicommutated flow analysis method for the determination of glyphosate, based on the quenching effect produced by this herbicide on the fluorescence of CdTe quantum dots (λexc/λem 400/548 nm/nm). The proposed analytical method presents detection and quantitation limits of 0.5 and 1.7 μg mL−1, respectively. A sample throughput of 30 samples per hour was achieved using a 2.5 mL min−1 flow rate. Interference studies were carried out to assess the selectivity of the method, observing no interference from other common pesticides. Finally, we carried out recovery experiments in water and cereal samples (amaranth, barley, oat, and quinoa). We obtained recovery yields between 92 and 108% in all the analyzed samples. HPLC-MS/MS was used as reference method to assess the accuracy of the proposed methodology.
KeywordsMulticommutation Pesticide Glyphosate Cereal Quantum dots
J.J.L. acknowledges research scholarship from Spanish Government (Ministerio de Educación y Ciencia) (FPU13/03869).
Compliance with Ethical Standards
Conflict of Interest
Julia Jiménez-López declares that she has no conflict of interest. Eulogio J. Llorent-Martínez declares that he has no conflict of interest. Pilar Ortega-Barrales declares that she has no conflict of interest. Antonio Ruiz-Medina declares that he has no conflict of interest.
This article does not contain any studies with human or animal subjects.
- Anastassiades M, Lehotay SJ, Štajnbaher D, Schenck FJ (2003) Fast and easy multiresidue method employing acetonitrile extraction/partitioning and ‘dispersive solid-phase extraction’ for the determination of pesticide residues in produce. J AOAC Int 86:412–431Google Scholar
- FAO, WHO (2006) Codex alimentarius. International food standards. Available at http://www.fao.org/fao-who-codexalimentarius/codex-texts/dbs/pestres/en/
- IARC (2015) Monographs, volume 112: some organophosphate insecticides and herbicides: tetrachlorvinphos, parathion, malathion, diazinon and glyphosate. IARC Monographs on the evaluation of carcinogenic risks to humans. IARC working group, LyonGoogle Scholar
- United States Environmental Protection Agency (2009) National primary drinking water regulations. EPA 816-F-09-004Google Scholar
- Williams GM, Aardema M, Acquavella J, Berry SC, Brusick D, Burns MM, de Camargo JLV, Garabrant D, Greim HA, Kier LD, Kirkland DJ, Marsh G, Solomon KR, Sorahan T, Roberts A, Weed DL (2016) A review of the carcinogenic potential of glyphosate by four independent expert panels and comparison to the IARC assessment. Crit Rev Toxicol 46:3–20CrossRefGoogle Scholar