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Food Analytical Methods

, Volume 11, Issue 5, pp 1321–1329 | Cite as

Evaluation of Freshness in Determination of Volatile Organic Compounds Released from Pork by HS-SPME-GC-MS

  • Yanwen Sun
  • Mangjuan Fu
  • Zhicheng Li
  • Xiaoli Peng
Article
  • 244 Downloads

Abstract

Freshness is an important parameter for evaluating the quality of pork, which has strong correlation with the characteristic volatile organic compounds (VOCs) in meat. In this study, the pork was stored at a refrigerator, and the VOCs were determined by using headspace solid-phase microextraction–gas chromatography–mass spectrometry (HS-SPME-GC-MS). The results of HS-SPME-GC-MS measurements were compared to total viable counts, total volatile basic nitrogen, and pH value to determine possible volatile spoilage markers. The pork was stored in a refrigerator up to 5 days at 4 °C and 466 days at −18 °C. The pork became stale at 4 °C when the 2,3-butanedione, 3-methyl butyraldehyde, 3-methyl-1-butanol, and acetoin increased to 26.44, 13.72, 71.56, and 340.48 μg/L, respectively. These substances can be seen as characteristic compounds of stale pork. The VOCs such as ethyl acetate, ethyl propionate, ethyl butyrate, and ethyl hexanoate had negative correlation (P < 0.05) with the pork freshness, which can be potentially considered as characteristic compounds of fresh pork. Therefore, the selected VOCs can be used as indicators for evaluation of pork freshness, and VOCs could be potentially applied in the development of intelligent refrigerators and refrigerated storehouse.

Keywords

Pork freshness VOCs HS-SPME-GC-MS Evaluation 

Notes

Funding

This study was partly supported by the Twelve-fifth Science and Technology Support Program (No. 2015BAD16B08); Government of PR China; the Science and Technology Program of Yangling Zone (No. 2016NY-30), Shaanxi Province; and the post-doctoral research station of Hefei Meiling Co., Ltd. (No. 20120731).

Compliance with Ethical Standards

Conflict of Interest

Yanwen Sun declares that she has no conflict of interest. Mangjuan Fu declares that she has no conflict of interest. Zhicheng Li declares that he has no conflict of interest. Xiaoli Peng declares that she has no conflict of interest.

Ethical Approval

This article does not contain any studies with human participants or animals performed by any of the authors.

Informed Consent

Not applicable.

References

  1. Alonso V, Muela E, Tenas J, Calanche JB, Roncalés P, Beltrán JA (2016) Changes in physicochemical properties and fatty acid composition of pork following long-term frozen storage. Eur Food Res Technol 242(12):2119–2127.  https://doi.org/10.1007/s00217-016-2708-y CrossRefGoogle Scholar
  2. AOAC (2002) Official methods of analysis, 17th edn. Association of Official Analytical Chemists, Washington, DCGoogle Scholar
  3. Balamatsia CC, Patsias A, Kontominas MG, Savvaidis IN (2007) Possible role of volatile amines as quality-indicating metabolites in modified atmosphere-packaged chicken fillets: correlation with microbiological and sensory attributes. Food Chem 104(4):1622–1628.  https://doi.org/10.1016/j.foodchem.2007.03.013 CrossRefGoogle Scholar
  4. Bermúdez R, Franco D, Carballo J, Lorenzo JM (2015) Influence of type of muscle on volatile compounds throughout the manufacture of Celta dry-cured ham. Food Sci Technol Int 21(8):581–592.  https://doi.org/10.1177/1082013214554935 CrossRefGoogle Scholar
  5. Bhattacharjee P, Panigrahi S, Lin D, Logue CM, Sherwood JS, Doetkott C, Marchello M (2011) A comparative qualitative study of the profile of volatile organic compounds associated with Salmonella contamination of packaged aged and fresh beef by HS-SPME/GC-MS. J Food Sci Technol 48(1):1–13.  https://doi.org/10.1007/s13197-010-0138-6 CrossRefGoogle Scholar
  6. Blixt Y, Borch E (2002) Comparison of shelf life of vacuum-packed pork and beef. Meat Sci 60(4):371–378.  https://doi.org/10.1016/S0309-1740(01)00145-0 CrossRefGoogle Scholar
  7. Cai J, Chen Q, Wan X, Zhao J (2011) Determination of total volatile basic nitrogen (TVB-N) content and Warner-Bratzler shear force (WBSF) in pork using Fourier transform near infrared (FT-NIR) spectroscopy. Food Chem 126(3):1354–1360.  https://doi.org/10.1016/j.foodchem.2010.11.098 CrossRefGoogle Scholar
  8. Cao Y, Gu W, Zhang J, Chu Y, Ye X, Hu Y, Chen J (2013) Effects of chitosan, aqueous extract of ginger, onion and garlic on quality and shelf life of stewed-pork during refrigerated storage. Food Chem 141(3):1655–1660.  https://doi.org/10.1016/j.foodchem.2013.04.084 CrossRefGoogle Scholar
  9. Chen Q, Hui Z, Zhao J, Ouyang Q (2014) Evaluation of chicken freshness using a low-cost colorimetric sensor array with AdaBoost–OLDA classification algorithm. LWT-Food Sci Technol 57(2):502–507.  https://doi.org/10.1016/j.lwt.2014.02.031 CrossRefGoogle Scholar
  10. Chulayo AY, Bradley G, Muchenje V (2016) Effects of transport distance, lairage time and stunning efficiency on cortisol, glucose, hspa1a and how they relate with meat quality in cattle. Meat Sci 117:89–96.  https://doi.org/10.1016/j.meatsci.2016.03.001 CrossRefGoogle Scholar
  11. Dang YL, Wang Z, Xu SY (2008) Methods for extracting the taste compounds from water soluble extract of Jinhua ham. Eur Food Res Technol 228(1):93–102.  https://doi.org/10.1007/s00217-008-0910-2 CrossRefGoogle Scholar
  12. Dima G, Tripodi G, Condurso C, Verzera A (2014) Volatile constituents of mini-watermelon fruits. J Essent Oil Res 26(5):323–327.  https://doi.org/10.1080/10412905.2014.933449 CrossRefGoogle Scholar
  13. Estévez M, Morcuende D, Sonia Ventanas A, Cava R (2003) Analysis of volatiles in meat from Iberian pigs and lean pigs after refrigeration and cooking by using SPME-GC-MS. J Agric Food Chem 51(11):3429–3435.  https://doi.org/10.1021/jf026218h CrossRefGoogle Scholar
  14. Fan W, Sun J, Chen Y, Qiu J, Zhang Y, Chi Y (2009) Effects of chitosan coating on quality and shelf life of silver carp during frozen storage. Food Chem 115(1):66–70.  https://doi.org/10.1016/j.foodchem.2008.11.060 CrossRefGoogle Scholar
  15. Fiorini D, Caprioli G, Sagratini G, Maggi F, Vittori S, Marcantoni E, Ballini R (2014) Quantitative profiling of volatile and phenolic substances in the wine vernaccia di Serrapetrona by development of an HS-SPME-GC-FID/MS method and HPLC-MS. Food Anal Method 7(8):1651–1660.  https://doi.org/10.1007/s12161-014-9802-1 CrossRefGoogle Scholar
  16. Hamoen JR, Vollebregt HM, Rg VDS (2013) Prediction of the time evolution of pH in meat. Food Chem 141(3):2363–2372.  https://doi.org/10.1016/j.foodchem.2013.04.127 CrossRefGoogle Scholar
  17. Han F, Huang X, Teye E, Gu F, Gu H (2013) Nondestructive detection of fish freshness during its preservation by combining electronic nose and electronic tongue techniques in conjunction with chemometric analysis. Anal Method 6(2):529–536CrossRefGoogle Scholar
  18. Huang L, Zhao J, Chen Q, Zhang Y (2013) Rapid detection of total viable count (TVC) in pork meat by hyperspectral imaging. Food Res Int 54(1):821–828.  https://doi.org/10.1016/j.foodres.2013.08.011 CrossRefGoogle Scholar
  19. Huang L, Zhao J, Chen Q, Zhang Y (2014) Nondestructive measurement of total volatile basic nitrogen (TVB-N) in pork meat by integrating near infrared spectroscopy, computer vision and electronic nose techniques. Food Chem 145(7):228–236.  https://doi.org/10.1016/j.foodchem.2013.06.073 CrossRefGoogle Scholar
  20. Jääskeläinen E, Johansson P, Kostiainen O, Nieminen T, Schmidt G, Somervuo P, Mohsinaa M, Vanninenb P, Auvinenc P, Björkrotha J (2013) Significance of heme-based respiration in meat spoilage caused by Leuconostoc gasicomitatum. Appl Env Microbiol 79(4):1078–1085.  https://doi.org/10.1128/AEM.02943-12 CrossRefGoogle Scholar
  21. Li X, Liu A, Ye R, Wang Y, Wang W (2015) Fabrication of gelatin-laponite composite films: effect of the concentration of laponite on physical properties and the freshness of meat during storage. Food Hydrocolloid 44:390–398.  https://doi.org/10.1016/j.foodhyd.2014.10.014 CrossRefGoogle Scholar
  22. Ma QL, Hamid N, Bekhit AED, Robertson J, Law TF (2013) Optimization of headspace solid phase microextraction (HS-SPME) for gas chromatography mass spectrometry (GC–MS) analysis of aroma compounds in cooked beef using response surface methodology. Microchem J 111:16–24.  https://doi.org/10.1016/j.microc.2012.10.007 CrossRefGoogle Scholar
  23. Mexis SF, Chouliara E, Kontominas MG (2012) Shelf life extension of ground chicken meat using an oxygen absorber and a citrus extract. LWT-Food Sci Technol 49(1):21–27.  https://doi.org/10.1016/j.lwt.2012.04.012 CrossRefGoogle Scholar
  24. Miyasaki T, Hamaguchi M, Yokoyama S (2011) Change of volatile compounds in fresh fish meat during ice storage. J Food Sci 76(9):C1319–C1325.  https://doi.org/10.1111/j.1750-3841.2011.02388.x CrossRefGoogle Scholar
  25. Nardini GS, Merib JO, Dias AN, Dutra JN, Silveira CD, Budziak D, Martendal E, Carasek E (2013) Determination of volatile profile of citrus fruit by HS-SPME/GC-MS with oxidized NiTi fibers using two temperatures in the same extraction procedure. Microchem J 109(7):128–133.  https://doi.org/10.1016/j.microc.2012.03.024 CrossRefGoogle Scholar
  26. Nieminen TT, Dalgaard P, Björkroth J (2016) Volatile organic compounds and Photobacterium phosphoreum, associated with spoilage of modified-atmosphere-packaged raw pork. Int J Food Microbiol 218:86–95.  https://doi.org/10.1016/j.ijfoodmicro.2015.11.003 CrossRefGoogle Scholar
  27. O’Mahony F, Green RA, Baylis C, Fernandes R, Papkovsky DB (2009) Analysis of total aerobic viable counts in samples of raw meat using fluorescence-based probe and oxygen consumption assay. Food Control 20(2):129–135.  https://doi.org/10.1016/j.foodcont.2008.03.003 CrossRefGoogle Scholar
  28. Olafsdóttir G, Martinsdóttir E, Oehlenschläger J, Dalgaard P, Jensen B, Undeland I, Mackie IM, Henehan G, Nielsen J, Nilsen H (1997) Methods to evaluate fish freshness in research and industry. Trends Food Sci Tech 8(8):258–265.  https://doi.org/10.1016/S0924-2244(97)01049-2 CrossRefGoogle Scholar
  29. Ordonez JA, Pablo BD, Castro BPD, Asensio MA, Sanz B (1991) Selected chemical and microbiological changes in refrigerated pork stored in carbon dioxide and oxygen enriched atmospheres. J Agr Food Chem 39(4):668–672.  https://doi.org/10.1021/jf00004a008 CrossRefGoogle Scholar
  30. Ortea I, Rodríguez A, Tabilomunizaga G, Pérezwon M, Aubourg SP (2010) Effect of hydrostatic high-pressure treatment on proteins, lipids and nucleotides in chilled farmed salmon (Oncorhynchus kisutch) muscle. Eur Food Res Technol 230(6):925–934.  https://doi.org/10.1007/s00217-010-1239-1 CrossRefGoogle Scholar
  31. Savell JW, Mueller SL, Baird BE (2005) The chilling of carcasses. Meat Sci 70(3):449–459.  https://doi.org/10.1016/j.meatsci.2004.06.027 CrossRefGoogle Scholar
  32. Shukla V, Kandeepan G, Vishnuraj MR (2015) Development of on-package indicator sensor for real-time monitoring of buffalo meat quality during refrigeration storage. Food Anal Method 8(6):1591–1597.  https://doi.org/10.1007/s12161-014-0066-6 CrossRefGoogle Scholar
  33. Soncin S, Chiesa LM, Cantoni C, Biondi PA (2007) Preliminary study of the volatile fraction in the raw meat of pork, duck and goose. J Food Compos Anal 20(5):436–439.  https://doi.org/10.1016/j.jfca.2006.09.001 CrossRefGoogle Scholar
  34. Song S, Zhang X, Hayat K, Liu P, Jia C, Xia S, Xiao Z, Tian H, Niu Y (2011) Formation of the beef flavour precursors and their correlation with chemical parameters during the controlled thermal oxidation of tallow. Food Chem 124(1):203–209.  https://doi.org/10.1016/j.foodchem.2010.06.010 CrossRefGoogle Scholar
  35. Song J, Smart RE, Dambergs RG, Sparrow AM, Wells RB, Wang H, Qian MC (2014) Pinot Noir wine composition from different vine vigour zones classified by remote imaging technology. Food Chem 153(12):52–59.  https://doi.org/10.1016/j.foodchem.2013.12.037 CrossRefGoogle Scholar
  36. Steiner WE, English WA (2015) Emerging trends in gas chromatography and mass spectrometry instrumentation for analytical & bioanalytical techniques. J Anal Bioanal Tech 6(1):1000e118Google Scholar
  37. Steingass CB, Grauwet T, Carle R (2014) Influence of harvest maturity and fruit logistics on pineapple (Ananas comosus (L.) Merr.) volatiles assessed by headspace solid phase microextraction and gas chromatography–mass spectrometry (HS-SPME-GC/MS). Food Chem 150(2):382–391.  https://doi.org/10.1016/j.foodchem.2013.10.092 CrossRefGoogle Scholar
  38. Tang Y, Zhang C, Cao S, Wang X, Qi H (2015) The effect of CmLOXs on the production of volatile organic compounds in four aroma types of melon (Cucumis melo). PLoS One 10(11):e0143567.  https://doi.org/10.1371/journal.pone.0143567 CrossRefGoogle Scholar
  39. Tao F, Peng Y (2015) A nondestructive method for prediction of total viable count in pork meat by hyperspectral scattering imaging. Food Bioprocess Tech 8(1):17–30.  https://doi.org/10.1007/s11947-014-1374-y CrossRefGoogle Scholar
  40. Wang D, Wang X, Liu T, Liu Y (2012) Prediction of total viable counts on chilled pork using an electronic nose combined with support vector machine. Meat Sci 90(2):373–377.  https://doi.org/10.1016/j.meatsci.2011.07.025 CrossRefGoogle Scholar
  41. Wierda RL, Fletcher G, Xu L, Dufour JP (2006) Analysis of volatile compounds as spoilage indicators in fresh king salmon (Oncorhynchus tshawytscha) during storage using SPME−GC−MS. J Agr Food Chem 54(22):8480–8490.  https://doi.org/10.1021/jf061377c CrossRefGoogle Scholar
  42. Xiao Y, Jin J, Hui G, Yin F, Wang M, Huang J, Ying X, Deng S (2014) Determination of the freshness of beef strip loins (M. longissimus lumborum) using electronic nose. Food Anal Method 7(8):1612–1618.  https://doi.org/10.1007/s12161-014-9796-8 CrossRefGoogle Scholar
  43. Zhang J, Yang Z, Yang Y, Han L, Yu Q, Cao H, Zhang W (2017) Development of a flavor fingerprint by GC-MS with chemometric method for volatile compounds of yak and yellow cattle bone soup. Food Anal Method 10(4):943–954.  https://doi.org/10.1007/s12161-016-0657-5 CrossRefGoogle Scholar
  44. Zhou J, Han Y, Zhuang H, Feng T, Xu B (2015) Influence of the type of extraction conditions and fiber coating on the meat of sauced duck neck volatile compounds extracted by solid-phase microextraction (SPME). Food Anal Method 8(7):1661–1672.  https://doi.org/10.1007/s12161-014-0049-7 CrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2017

Authors and Affiliations

  • Yanwen Sun
    • 1
  • Mangjuan Fu
    • 1
  • Zhicheng Li
    • 1
  • Xiaoli Peng
    • 1
  1. 1.College of Food Science and EngineeringNorthwest A&F UniversityYanglingPeople’s Republic of China

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