Partition Behaviors of Different Polar Anthocyanins in Aqueous Two-Phase Systems and Extraction of Anthocyanins from Nitraria tangutorun Bobr. and Lycium ruthenicum Murr.
This study aimed to investigate the partition behaviors of various polar anthocyanins in NaH2PO4/(NH4)2SO4-ethanol aqueous two-phase systems (ATPS) and to extract anthocyanins from Nitraria tangutorun Bobr. and Lycium ruthenicum Murr. Anthocyanins in Hibiscus sabdariffa L., Morus atropurpurea Roxb., N. tangutorun, and L. ruthenicum were profiled using HPLC-ESI-MS/MS and HPLC-DAD, and the partition behaviors of total anthocyanins and main anthocyanins were studied. The partition coefficient of anthocyanins increased with increased hydrophobicity, and low-polarity anthocyanins exhibited a higher preference for the top phase in NaH2PO4/(NH4)2SO4-ethanol ATPS. Additionally, the NaH2PO4-ethanol ATPS gave higher selectivity and total anthocyanin yield than the (NH4)2SO4-ethanol system. Extraction at 65 °C for 45 min and at 45.5 °C for 45 min using 28% NaH2PO4 and 26% ethanol (w/w) led to the recovery of 98.91 ± 0.03% of N. tangutorun anthocyanins (3.62 ± 0.05 mg/g) and 99.84 ± 0.01% of L. ruthenicum anthocyanins (13.16 ± 0.29 mg/g) from raw material; more than 70% of total sugars were removed in a single step. NaH2PO4-ethanol aqueous two-phase extraction is a promising method for extracting anthocyanins from N. tangutorun and L. ruthenicum.
KeywordsAnthocyanins Aqueous two-phase extraction HPLC-ESI-MS/MS Response surface methodology Natural pigments Food analysis Food composition
We thank Prof. Zhe-zhi Wang for his generosity in sharing his lab facilities.
This work was supported by the Fundamental Research Funds for the Central Universities [grant number GK201303009] and Science and Technology Cooperation Project between Shaanxi Province and Qinghai Province [grant number 2014SJ-07].
Compliance with Ethical Standards
Conflict of Interest
Jun Sang declares that he has no conflict of interest. Kai-kai Dang declares that he has no conflict of interest. Qun Ma declares that she has no conflict of interest. Bing Li declares that she has no conflict of interest. Ya-ya Huang declares that she has no conflict of interest. Cui-qin Li declares that she has no conflict of interest.
This article does not contain any studies with human participants or animals performed by any of the authors.
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