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Food Analytical Methods

, Volume 11, Issue 5, pp 1312–1320 | Cite as

Simultaneous Determination of Pyraclostrobin, Prochloraz, and its Metabolite in Apple and Soil Via RRLC-MS/MS

  • Xueqi Fan
  • Shengming Zhao
  • Xiaoxin Chen
  • Jiye Hu
Article
  • 149 Downloads

Abstract

A sensitive and high-efficiency method for simultaneous detection of pyraclostrobin, prochloraz, and its metabolite in apple and soil based on QuEChERS pretreatment combined with rapid resolution liquid chromatography tandem mass spectrometry was established and validated. The limits of quantification of three compounds in two matrixes were 0.005 mg kg−1. The average recoveries of pyraclostrobin, prochloraz, and 2,4,6-trichlorophenol in soil matrix were in the ranges of 87–105%, 86–95%, and 90–96%, respectively, with all the relative standard deviations (RSDs) of < 9.6%, while recoveries were 89–93%, 83–97%, and 89–101% in apple with the RSDs of < 6.5% at three spiking levels. For verify the applicability of this method, the real samples from three representative locations were detected. The degradation behaviors and residue distributions of pyraclostrobin and prochloraz and its metabolite in apple ecosystem were investigated. The field experiment data showed that the dissipation of pyraclostrobin and prochloraz in apple and soil followed pseudo-first-order kinetic models. The half-lives of pyraclostrobin in soil and apple were 8.6–19.8 and 7.9–15.1 days, while prochloraz were 8.9–21 and 5.8–12.4 days. The highest terminal residue of total prochloraz and pyraclostrobin in apple, after spraying three to four times with the interval of 28 days, was far below the maximum residue limits recommended by China. This research could provide guidance on a reasonable usage of prochloraz and pyraclostrobin in apple orchard.

Keywords

Pyraclostrobin Prochloraz Metabolite Apple QuEChERS method RRLC-MS/MS 

Abbreviations

LODs

Limits of detection

LOQs

Limits of quantification

MRLs

Maximum residue limits

PHI

Pre-harvest interval

RRLC-MS/MS

Rapid resolution liquid chromatography tandem mass spectrometry

RSDs

Relative standard deviations

EW

Emulsion in water

Notes

Funding

This study was funded by the National Natural Science Foundation of China (grant no. 21677009) and the Natural Science Foundation of Beijing (grant no. 8162029).

Compliance with Ethical Standards

Conflict of Interest

X.F. declares that she has no conflict of interest. S.Z. declares that he has no conflict of interest. X.C. declares that she has no conflict of interest. J.H. declares that she has no conflict of interest.

Ethical Approval

This article does not contain any studies with human participants or animals performed by any of the authors.

Informed Consent

Informed consent was obtained from all individual participants included in the study.

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Copyright information

© Springer Science+Business Media, LLC 2017

Authors and Affiliations

  • Xueqi Fan
    • 1
  • Shengming Zhao
    • 1
  • Xiaoxin Chen
    • 1
  • Jiye Hu
    • 1
  1. 1.College of Chemistry and Biological EngineeringUniversity of Science and Technology BeijingBeijingPeople’s Republic of China

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