Food Analytical Methods

, Volume 10, Issue 6, pp 2046–2052 | Cite as

Rapid Determination of Toxic Rhodamine B in Food Samples Using Exfoliated Graphene-Modified Electrode



Due to being harmful to the human health, the qualitative and quantitative determination of rhodamine B (RhB) is of great significance. In this work, a sensitive, rapid, and convenient electrochemical method was developed for the determination of RhB based on the enhancement effects of N-methyl-2-pyrrolidone (NMP) exfoliated graphene nanosheets (GS). On the surface of NMP-exfoliated GS-modified electrode, the oxidation signals of RhB are enhanced greatly, and consequently the detection sensitivity is obviously improved. The influences of pH value, GS modification amount, and accumulation time were studied. The linear range of this new method is from 5 to 120 nM, and the detection limit is 1.5 nM. It was used in different soy sauce samples, and the detected results consisted with the values that are obtained by high-performance liquid chromatography.


Rhodamine B Rapid determination Electrochemical sensing Exfoliated graphene 



This work was granted by the Opening Project of Zhejiang Provincial Top Key Discipline of Pharmaceutical Sciences.

Compliance with Ethical Standards


This study was supported by the Opening Project of Zhejiang Provincial Top Key Discipline of Pharmaceutical Sciences.

Conflict of Interest

Dong Sun declares no conflict of interest. Xiaofeng Yang declares no conflict of interest.

Ethical Approval

This article does not contain any studies with human participants or animals performed by any of the authors.

Informed Consent

Not applicable.


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Copyright information

© Springer Science+Business Media New York 2016

Authors and Affiliations

  1. 1.School of Pharmaceutical SciencesWenzhou Medical UniversityWenzhouChina

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