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Food Analytical Methods

, Volume 10, Issue 6, pp 2011–2017 | Cite as

Development of an HPLC-DAD Method for the Determination of Five Sulfonamides in Shrimps and Validation According to the European Decision 657/2002/EC

  • Stamatia Charitonos
  • Victoria F. Samanidou
  • Ioannis Papadoyannis
Article

Abstract

A method for the determination of five sulfonamide drugs: sulfadiazine (SDZ), sulfamethazine (SMTH), sulfisoxazole (SIX), sulfadimethoxine (SDMX), sulfaquinoxaline (SQX) in shrimps was developed using a high-pressure liquid chromatography–diode array detection (HPLC–DAD) instrumentation. The drugs were extracted from the samples using a solid-liquid extraction, with a mixture of acetonitrile-methanol and aqueous solution of acetic acid. The separation was achieved with LiChroCART-LiChrospher® 100 RP-18 (5 μm, 250 × 4 mm), and the mobile phase consisted of (A) aqueous solution of acetic acid 0.1% (65%) and (B) mixture of ACN-MeOH 50:50 v/v (35%), delivered at a flow rate of 0.8 mL/min isocratically. A DAD detector was used for the detection of the sulfonamides at 265 nm. The method was validated according to the 657/2002/EC decision of the European Union. The recoveries for within-day repeatability were 90.2–109.0% and for between-day precision 88.6–108.4%. The limit of quantitation for all five suflonamides was 50 μg/kg, and the limit of detection was 15 μg/kg.

Keywords

Sulfonamides Shrimps Antibiotics Sample preparation HPLC 

Notes

Compliance with Ethical Standards

Funding

No funding was received for the study.

Conflict of Interest

Stamatia Charitonos declares that she has no conflict of interest. Victoria F. Samanidou declares that she has no conflict of interest. Ioannis Papadoyannis declares that he has no conflict of interest.

Ethical Approval

This article does not contain any studies with human participants or animals performed by any of the authors.

Informed Consent

Informed consent was obtained from all individual participants included in the study.

References

  1. Aronson JK (2006) Meyler’s side effects of drugs: the international encyclopedia of adverse drug reactions and interactions. Meyler’s Side Effects of Drugs: The International Encyclopedia of Adverse Drug Reactions and Interactions:2248–2249. doi: 10.1016/B0-44-451005-2/01277-8
  2. Dang PK, Degand G, Danyi S, Pierret G, Delahaut P, Ton VD, Maghuin-Rogister G, Scippo ML (2010) Validation of a two-plate microbiological method for screening antibiotic residues in shrimp tissue. Anal Chim Acta 672(1–2) Elsevier B.V.:30–39. doi: 10.1016/j.aca.2010.03.055 CrossRefGoogle Scholar
  3. Decision, 96/23/Ec Commission (2002) 96/23/EC COMMISSION DECISION of 12 August 2002 implementing council directive 96/23/EC concerning the performance of analytical methods and the interpretation of results (notified under document number C (2002) 3044) (text with EEA relevance) (2002/657/EC). 96/23/Ec Commission Decision 29. doi: 10.1017/CBO9781107415324.004
  4. Tolika EP, Samanidou VF, Papadoyannis IN (2012) Development and validation of an HPLC method for the simultaneous determination of ten sulfonamide residues in bovine, porcine and chicken tissues according to 2002/657/EC. Curr Pharm Anal 8(1):56–67. doi: 10.2174/157341212798995476 CrossRefGoogle Scholar
  5. Samanidou V, Bitas D, Charitonos S, Papadoyannis I (2016) On the extraction of antibiotics from shrimps prior to chromatographic analysis. Separations 3(1):8. doi: 10.3390/chromatography3010008 Google Scholar
  6. Shi X, Meng Y, Liu J, Sun A, Li D, Yao C, Lu Y, Chen J (2011) Group-selective molecularly imprinted polymer solid-phase extraction for the simultaneous determination of six sulfonamides in aquaculture products. J Chromatogr B 879(15–16) Elsevier B.V.:1071–1076. doi: 10.1016/j.jchromb.2011.03.019 CrossRefGoogle Scholar
  7. Storey JM, Clark SB, Johnson AS, Andersen WC, Turnipseed SB, Lohne JJ, Burger RJ, Ayres PR, Carr JR, Madson MR (2014) Analysis of sulfonamides, trimethoprim, fluoroquinolones, quinolones, triphenylmethane dyes and Methyltestosterone in fish and shrimp using liquid chromatography-mass spectrometry. J Chromatogr B Anal Technol Biomed Life Sci 972 :38–47. doi: 10.1016/j.jchromb.2014.09.009Elsevier B.V.CrossRefGoogle Scholar
  8. Tolika EP, Samanidou VF, Papadoyannis IN (2011a) Development and validation of an HPLC method for the determination of ten sulfonamide residues in milk according to 2002/657/EC. J Sep Sci 34(14):1627–1635CrossRefGoogle Scholar
  9. Tolika EP, Samanidou VF, Papadoyannis IN (2011b) Development and validation of an HPLC method for the simultaneous determination of ten sulfonamide residues in whole egg according to 2002/657/Ec. J Liq Chromatogr Relat Technol 34(19):2396–2410. doi: 10.1080/10826076.2011.591021 CrossRefGoogle Scholar
  10. Won SY, Lee CH, Chang HS, Kim SO, Lee SH, Kim DS (2011) Monitoring of 14 sulfonamide antibiotic residues in marine products using HPLC-PDA and LC-MS/MS. Food Control 22(7) Elsevier Ltd:1101–1107. doi: 10.1016/j.foodcont.2011.01.005 CrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media New York 2016

Authors and Affiliations

  • Stamatia Charitonos
    • 1
  • Victoria F. Samanidou
    • 1
  • Ioannis Papadoyannis
    • 1
  1. 1.Laboratory of Analytical Chemistry, Department of ChemistryAristotle University of ThessalonikiThessalonikiGreece

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