Development of an HPLC-DAD Method for the Determination of Five Sulfonamides in Shrimps and Validation According to the European Decision 657/2002/EC
A method for the determination of five sulfonamide drugs: sulfadiazine (SDZ), sulfamethazine (SMTH), sulfisoxazole (SIX), sulfadimethoxine (SDMX), sulfaquinoxaline (SQX) in shrimps was developed using a high-pressure liquid chromatography–diode array detection (HPLC–DAD) instrumentation. The drugs were extracted from the samples using a solid-liquid extraction, with a mixture of acetonitrile-methanol and aqueous solution of acetic acid. The separation was achieved with LiChroCART-LiChrospher® 100 RP-18 (5 μm, 250 × 4 mm), and the mobile phase consisted of (A) aqueous solution of acetic acid 0.1% (65%) and (B) mixture of ACN-MeOH 50:50 v/v (35%), delivered at a flow rate of 0.8 mL/min isocratically. A DAD detector was used for the detection of the sulfonamides at 265 nm. The method was validated according to the 657/2002/EC decision of the European Union. The recoveries for within-day repeatability were 90.2–109.0% and for between-day precision 88.6–108.4%. The limit of quantitation for all five suflonamides was 50 μg/kg, and the limit of detection was 15 μg/kg.
KeywordsSulfonamides Shrimps Antibiotics Sample preparation HPLC
Compliance with Ethical Standards
No funding was received for the study.
Conflict of Interest
Stamatia Charitonos declares that she has no conflict of interest. Victoria F. Samanidou declares that she has no conflict of interest. Ioannis Papadoyannis declares that he has no conflict of interest.
This article does not contain any studies with human participants or animals performed by any of the authors.
Informed consent was obtained from all individual participants included in the study.
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